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ID   PSG927     preliminary; circular DNA; SYN; 5634 BP.
XX
AC   U14120;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG927 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5634
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5634
RC   pSG927
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537465
CC   NM (pSG927)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG927 5634bp"
FT   CDS             complement(1987..2847)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2947..5475
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5634 BP; 1492 A; 1293 C; 1319 G; 1530 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt
     ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     ggcgacacgg aaatgttgaa tactcatact cttccttttt caatgatctc ctgatgactg
     actcactgat aataaaaata cggcttcaga atttctcaag actacactca ctgtccgact
     tcaagtatga catttccctt gctacctgca tacgcaagtg ttgcagagtt tgataattcc
     ttgagtttgg taggaaaagc cgtgtttccc tatgctgctg accagctgca caacctgatc
     aagttcactc aatcgactga gcttcaagtt aatgtgcaag ttgagtcatc cgttacagag
     gaccaatttg aggagctgat cgacaacttg ctcaagttgt acaataatgg tatcaatgaa
     gtgattttgg acctagattt ggcagaaaga gttgtccaaa ggatcccagg cgctagggtt
     atctatagga ccctggttga taaagttgca tccttgcccg ctaatgctag tatcgctgtg
     cctttttctt ctccactggg cgatttgaaa agtttcacta atggcggtag tagaactgtt
     tatgcttttt ctgagaccgc aaagttggta gatgtgactt ccactgttgc ttctggtata
     atccccatta ttgatgctcg gcaattgact actgaatacg aactttctga agatgtcaaa
     aagttccctg tcagtgaaat tttgttggcg tctttgacta ctgaccgccc cgatggtcta
     ttcactactt tggtggctga ctcttctaat tactcgttgg gcctggtgta ctcgtccaaa
     aagtctattc cggaggctat aaggacacaa actggagtct accaatctcg tcgtcacggt
     ttgtggtata aaggtgctac atctggagca actcaaaagt tgctgggtat cgaattggat
     tgtgatggag actgcttgaa atttgtggtt gaacaaacag gtgttggttt ctgtcacttg
     gaacgcactt cctgttttgg ccaatcaaag ggtcttagag ccatggaagc caccttgtgg
     gatcgtaaga gcaatgctcc agaaggttct tataccaaac ggttatttga cgacgaagtt
     ttgttgaacg ctaaaattag ggaggaagct gatgaacttg cagaagctaa atccaaggaa
     gatatagcct gggaatgtgc tgacttattt tattttgcat tagttagatg tgccaagtac
     ggcgtgacgt tggacgaggt ggagagaaac ctggatatga agtccctaaa ggtcactaga
     aggaaaggag atgccaagcc aggatacacc aaggaacaac ctaaagaaga atccaaacct
     aaagaagtcc cttctgaagg tcgtattgaa ttgtgcaaaa ttgacgtttc taaggcctcc
     tcacaagaaa ttgaagatgc ccttcgtcgt cctatccaga aaacggaaca gattatggaa
     ttagtcaaac caattgtcga caatgttcgt caaaatggtg acaaagccct tttagaacta
     actgccaagt ttgatggagt cgctttgaag acacctgtgt tagaagctcc tttcccagag
     gaacttatgc aattgccaga taacgttaag agagccattg atctctctat agataacgtc
     aggaaattcc atgaagctca actagcggag acgttgcaag ttgagacttg ccctggtgta
     gtctgctctc gttttgcaag acctattgag aaagttggcc tctatattcc tggtggaacc
     gcaattctgc cttccacttc cctgatgctg ggtgttcctg ccaaagttgc tggttgcaaa
     gaaattgttt ttgcatctcc acctaagaag gatggtaccc ttaccccaga agtcatctac
     gttgcccaca aggttggtgc taagtgtatc gtgctagcag gaggcgccca ggcagtagct
     gctatggctt acggaacaga aactgttcct aagtgtgaca aaatatttgg tccaggaaac
     cagttcgtta ctgctgccaa gatgatggtt caaaatgaca catcagccct gtgtagtatt
     gacatgcctg ctgggccttc tgaagttcta gttattgctg ataaatacgc tgatccagat
     ttcgttgtct cagaccttct gtctcaagct gaacatggta ttgattccca ggtgattctg
     ttggctgtcg atatgacaga caaggagctt gccagaattg aagatgctgt tcacaaccaa
     gctgtgcagt tgccaagggt tgaaattgta cgcaagtgta ttgcacactc tacaacccta
     tcggttgcaa cctacgagca ggctttggaa atgtccaatc agtacgctcc tgaacacttg
     atcctgcaaa tcgagaatgc ttcttatgtt gatcaagtac aacacgctgg atctgtgttt
     gttggtgcct actctccaga gagttgtgga gattactcct ccggtaccaa ccacactttg
     ccaacgtacg gatatgcccg tcaatacagc ggagttaaca ctgcaacctt ccagaagttc
     atcacttcac aagacgtaac tcctgaggga ctgaaacata ttggccaagc agtgatggat
     ctggctgctg ttgaaggtct agatgctcac cgcaatgctg ttaaggttcg tatggagaaa
     ctgggactta tttaattatt tagagatttt aacttacatt tagattcgat agatcattat
     tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//