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ID   PSG928     preliminary; circular DNA; SYN; 5622 BP.
XX
AC   U14121;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG928 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5622
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5622
RC   pSG928
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537468
CC   NM (pSG928)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG928 5622bp"
FT   CDS             complement(1975..2835)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2935..5463
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5622 BP; 1496 A; 1288 C; 1312 G; 1526 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     ttgggcccga cgtcgcatgc tcccggccgc catggccgcg ggatatcact agtgcggccg
     cctgcaggtc gaccatatgg gagagctccc aacgcgttgg atgcatagct tgagtattct
     atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atgatctcct gatgactgac tcactgataa
     taaaaatacg gcttcagaat ttctcaagac tacactcact gtccgacttc aagtatgaca
     tttcccttgc tacctgcata cgcaagtgtt gcagagtttg ataattcctt gagtttggta
     ggaaaagccg tgtttcccta tgctgctgac cagctgcaca acctgatcaa gttcactcaa
     tcgactgagc ttcaagttaa tgtgcaagtt gagtcatccg ttacagagga ccaatttgag
     gagctgatcg acaacttgct caagttgtac aataatggta tcaatgaagt gattttggac
     ctagatttgg cagaaagagt tgtccaaagg atcccaggcg ctagggttat ctataggacc
     ctggttgata aagttgcatc cttgcccgct aatgctagta tcgctgtgcc tttttcttct
     ccactgggcg atttgaaaag tttcactaat ggcggtagta gaactgttta tgctttttct
     gagaccgcaa agttggtaga tgtgacttcc actgttgctt ctggtataat ccccattatt
     gatgctcggc aattgactac tgaatacgaa ctttctgaag atgtcaaaaa gttccctgtc
     agtgaaattt tgttggcgtc tttgactact gaccgccccg atggtctatt cactactttg
     gtggctgact cttctaatta ctcgttgggc ctggtgtact cgtccaaaaa gtctattccg
     gaggctataa ggacacaaac tggagtctac caatctcgtc gtcacggttt gtggtataaa
     ggtgctacat ctggagcaac tcaaaagttg ctgggtatcg aattggattg tgatggagac
     tgcttgaaat ttgtggttga acaaacaggt gttggtttct gtcacttgga acgcacttcc
     tgttttggcc aatcaaaggg tcttagagcc atggaagcca ccttgtggga tcgtaagagc
     aatgctccag aaggttctta taccaaacgg ttatttgacg acgaagtttt gttgaacgct
     aaaattaggg aggaagctga tgaacttgca gaagctaaat ccaaggaaga tatagcctgg
     gaatgtgctg acttatttta ttttgcatta gttagatgtg ccaagtacgg cgtgacgttg
     gacgaggtgg agagaaacct ggatatgaag tccctaaagg tcactagaag gaaaggagat
     gccaagccag gatacaccaa ggaacaacct aaagaagaat ccaaacctaa agaagtccct
     tctgaaggtc gtattgaatt gtgcaaaatt gacgtttcta aggcctcctc acaagaaatt
     gaagatgccc ttcgtcgtcc tatccagaaa acggaacaga ttatggaatt agtcaaacca
     attgtcgaca atgttcgtca aaatggtgac aaagcccttt tagaactaac tgccaagttt
     gatggagtcg ctttgaagac acctgtgtta gaagctcctt tcccagagga acttatgcaa
     ttgccagata acgttaagag agccattgat ctctctatag ataacgtcag gaaattccat
     gaagctcaac tagcggagac gttgcaagtt gagacttgcc ctggtgtagt ctgctctcgt
     tttgcaagac ctattgagaa agttggcctc tatattcctg gtggaaccgc aattctgcct
     tccacttccc tgatgctggg tgttcctgcc aaagttgctg gttgcaaaga aattgttttt
     gcatctccac ctaagaagga tggtaccctt accccagaag tcatctacgt tgcccacaag
     gttggtgcta agtgtatcgt gctagcagga ggcgcccagg cagtagctgc tatggcttac
     ggaacagaaa ctgttcctaa gtgtgacaaa atatttggtc caggaaacca gttcgttact
     gctgccaaga tgatggttca aaatgacaca tcagccctgt gtagtattga catgcctgct
     gggccttctg aagttctagt tattgctgat aaatacgctg atccagattt cgttgtctca
     gaccttctgt ctcaagctga acatggtatt gattcccagg tgattctgtt ggctgtcgat
     atgacagaca aggagcttgc cagaattgaa gatgctgttc acaaccaagc tgtgcagttg
     ccaagggttg aaattgtacg caagtgtatt gcacactcta caaccctatc ggttgcaacc
     tacgagcagg ctttggaaat gtccaatcag tacgctcctg aacacttgat cctgcaaatc
     gagaatgctt cttatgttga tcaagtacaa cacgctggat ctgtgtttgt tggtgcctac
     tctccagaga gttgtggaga ttactcctcc ggtaccaacc acactttgcc aacgtacgga
     tatgcccgtc aatacagcgg agttaacact gcaaccttcc agaagttcat cacttcacaa
     gacgtaactc ctgagggact gaaacatatt ggccaagcag tgatggatct ggctgctgtt
     gaaggtctag atgctcaccg caatgctgtt aaggttcgta tggagaaact gggacttatt
     taattattta gagattttaa cttacattta gattcgatag atcattattg aagcatttat
     cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//