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ID   PSG929     preliminary; circular DNA; SYN; 5619 BP.
XX
AC   U14119;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG929 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5619
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5619
RC   pSG929
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537462
CC   NM (pSG929)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG929 5619bp"
FT   CDS             complement(1972..2832)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2932..5460
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5619 BP; 1500 A; 1282 C; 1307 G; 1530 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     ttgggcccga cgtcgcatgc tcctctagac tcgaggaatt cggtaccccg ggttcgaaat
     cgataagctt ggatccggag agctcccaac gcgttggatg catagcttga gtattctata
     gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     ttgaatactc atactcttcc tttttcaatg atctcctgat gactgactca ctgataataa
     aaatacggct tcagaatttc tcaagactac actcactgtc cgacttcaag tatgacattt
     cccttgctac ctgcatacgc aagtgttgca gagtttgata attccttgag tttggtagga
     aaagccgtgt ttccctatgc tgctgaccag ctgcacaacc tgatcaagtt cactcaatcg
     actgagcttc aagttaatgt gcaagttgag tcatccgtta cagaggacca atttgaggag
     ctgatcgaca acttgctcaa gttgtacaat aatggtatca atgaagtgat tttggaccta
     gatttggcag aaagagttgt ccaaaggatc ccaggcgcta gggttatcta taggaccctg
     gttgataaag ttgcatcctt gcccgctaat gctagtatcg ctgtgccttt ttcttctcca
     ctgggcgatt tgaaaagttt cactaatggc ggtagtagaa ctgtttatgc tttttctgag
     accgcaaagt tggtagatgt gacttccact gttgcttctg gtataatccc cattattgat
     gctcggcaat tgactactga atacgaactt tctgaagatg tcaaaaagtt ccctgtcagt
     gaaattttgt tggcgtcttt gactactgac cgccccgatg gtctattcac tactttggtg
     gctgactctt ctaattactc gttgggcctg gtgtactcgt ccaaaaagtc tattccggag
     gctataagga cacaaactgg agtctaccaa tctcgtcgtc acggtttgtg gtataaaggt
     gctacatctg gagcaactca aaagttgctg ggtatcgaat tggattgtga tggagactgc
     ttgaaatttg tggttgaaca aacaggtgtt ggtttctgtc acttggaacg cacttcctgt
     tttggccaat caaagggtct tagagccatg gaagccacct tgtgggatcg taagagcaat
     gctccagaag gttcttatac caaacggtta tttgacgacg aagttttgtt gaacgctaaa
     attagggagg aagctgatga acttgcagaa gctaaatcca aggaagatat agcctgggaa
     tgtgctgact tattttattt tgcattagtt agatgtgcca agtacggcgt gacgttggac
     gaggtggaga gaaacctgga tatgaagtcc ctaaaggtca ctagaaggaa aggagatgcc
     aagccaggat acaccaagga acaacctaaa gaagaatcca aacctaaaga agtcccttct
     gaaggtcgta ttgaattgtg caaaattgac gtttctaagg cctcctcaca agaaattgaa
     gatgcccttc gtcgtcctat ccagaaaacg gaacagatta tggaattagt caaaccaatt
     gtcgacaatg ttcgtcaaaa tggtgacaaa gcccttttag aactaactgc caagtttgat
     ggagtcgctt tgaagacacc tgtgttagaa gctcctttcc cagaggaact tatgcaattg
     ccagataacg ttaagagagc cattgatctc tctatagata acgtcaggaa attccatgaa
     gctcaactag cggagacgtt gcaagttgag acttgccctg gtgtagtctg ctctcgtttt
     gcaagaccta ttgagaaagt tggcctctat attcctggtg gaaccgcaat tctgccttcc
     acttccctga tgctgggtgt tcctgccaaa gttgctggtt gcaaagaaat tgtttttgca
     tctccaccta agaaggatgg tacccttacc ccagaagtca tctacgttgc ccacaaggtt
     ggtgctaagt gtatcgtgct agcaggaggc gcccaggcag tagctgctat ggcttacgga
     acagaaactg ttcctaagtg tgacaaaata tttggtccag gaaaccagtt cgttactgct
     gccaagatga tggttcaaaa tgacacatca gccctgtgta gtattgacat gcctgctggg
     ccttctgaag ttctagttat tgctgataaa tacgctgatc cagatttcgt tgtctcagac
     cttctgtctc aagctgaaca tggtattgat tcccaggtga ttctgttggc tgtcgatatg
     acagacaagg agcttgccag aattgaagat gctgttcaca accaagctgt gcagttgcca
     agggttgaaa ttgtacgcaa gtgtattgca cactctacaa ccctatcggt tgcaacctac
     gagcaggctt tggaaatgtc caatcagtac gctcctgaac acttgatcct gcaaatcgag
     aatgcttctt atgttgatca agtacaacac gctggatctg tgtttgttgg tgcctactct
     ccagagagtt gtggagatta ctcctccggt accaaccaca ctttgccaac gtacggatat
     gcccgtcaat acagcggagt taacactgca accttccaga agttcatcac ttcacaagac
     gtaactcctg agggactgaa acatattggc caagcagtga tggatctggc tgctgttgaa
     ggtctagatg ctcaccgcaa tgctgttaag gttcgtatgg agaaactggg acttatttaa
     ttatttagag attttaactt acatttagat tcgatagatc attattgaag catttatcag
     ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     gttccgcgca catttccccg aaaagtgcca cctgacgtc
//