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ID   PSG930   preliminary; circular DNA; SYN; 5621 BP.
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AC   U14118;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG930 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5621
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5621
RC   pSG930 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537459
CC   NM (pSG930)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG930 5621bp"
FT   CDS             complement(1974..2834)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2934..5462
FT                   /note="GEN histidinol dehydrogenase gene"
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SQ   Sequence 5621 BP; 1497 A; 1282 C; 1310 G; 1532 T; 0 other;
     cgaccaatta cccctactac gttggaacat agtccgtccc cgggggaaga tttattgtct
     caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta atttagcttg
     actacgatgc atataatttt aattttattt taaattatat atgaggtaag agtataactc
     taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat taattacaac
     taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca gatagaaacc
     atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg aacagtcaaa
     cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat atgcggtgtg
     aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc
     tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttgggccctc
     tagatgcatg ctcgagcggc cgccagtgtg atggatatct gcagaattcc agcacactgg
     cggccgttac tagtggatcc gagctcggta ccaagcttga tgcatagctt gagtattcta
     tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     tgttgaatac tcatactctt cctttttcaa tgatctcctg atgactgact cactgataat
     aaaaatacgg cttcagaatt tctcaagact acactcactg tccgacttca agtatgacat
     ttcccttgct acctgcatac gcaagtgttg cagagtttga taattccttg agtttggtag
     gaaaagccgt gtttccctat gctgctgacc agctgcacaa cctgatcaag ttcactcaat
     cgactgagct tcaagttaat gtgcaagttg agtcatccgt tacagaggac caatttgagg
     agctgatcga caacttgctc aagttgtaca ataatggtat caatgaagtg attttggacc
     tagatttggc agaaagagtt gtccaaagga tcccaggcgc tagggttatc tataggaccc
     tggttgataa agttgcatcc ttgcccgcta atgctagtat cgctgtgcct ttttcttctc
     cactgggcga tttgaaaagt ttcactaatg gcggtagtag aactgtttat gctttttctg
     agaccgcaaa gttggtagat gtgacttcca ctgttgcttc tggtataatc cccattattg
     atgctcggca attgactact gaatacgaac tttctgaaga tgtcaaaaag ttccctgtca
     gtgaaatttt gttggcgtct ttgactactg accgccccga tggtctattc actactttgg
     tggctgactc ttctaattac tcgttgggcc tggtgtactc gtccaaaaag tctattccgg
     aggctataag gacacaaact ggagtctacc aatctcgtcg tcacggtttg tggtataaag
     gtgctacatc tggagcaact caaaagttgc tgggtatcga attggattgt gatggagact
     gcttgaaatt tgtggttgaa caaacaggtg ttggtttctg tcacttggaa cgcacttcct
     gttttggcca atcaaagggt cttagagcca tggaagccac cttgtgggat cgtaagagca
     atgctccaga aggttcttat accaaacggt tatttgacga cgaagttttg ttgaacgcta
     aaattaggga ggaagctgat gaacttgcag aagctaaatc caaggaagat atagcctggg
     aatgtgctga cttattttat tttgcattag ttagatgtgc caagtacggc gtgacgttgg
     acgaggtgga gagaaacctg gatatgaagt ccctaaaggt cactagaagg aaaggagatg
     ccaagccagg atacaccaag gaacaaccta aagaagaatc caaacctaaa gaagtccctt
     ctgaaggtcg tattgaattg tgcaaaattg acgtttctaa ggcctcctca caagaaattg
     aagatgccct tcgtcgtcct atccagaaaa cggaacagat tatggaatta gtcaaaccaa
     ttgtcgacaa tgttcgtcaa aatggtgaca aagccctttt agaactaact gccaagtttg
     atggagtcgc tttgaagaca cctgtgttag aagctccttt cccagaggaa cttatgcaat
     tgccagataa cgttaagaga gccattgatc tctctataga taacgtcagg aaattccatg
     aagctcaact agcggagacg ttgcaagttg agacttgccc tggtgtagtc tgctctcgtt
     ttgcaagacc tattgagaaa gttggcctct atattcctgg tggaaccgca attctgcctt
     ccacttccct gatgctgggt gttcctgcca aagttgctgg ttgcaaagaa attgtttttg
     catctccacc taagaaggat ggtaccctta ccccagaagt catctacgtt gcccacaagg
     ttggtgctaa gtgtatcgtg ctagcaggag gcgcccaggc agtagctgct atggcttacg
     gaacagaaac tgttcctaag tgtgacaaaa tatttggtcc aggaaaccag ttcgttactg
     ctgccaagat gatggttcaa aatgacacat cagccctgtg tagtattgac atgcctgctg
     ggccttctga agttctagtt attgctgata aatacgctga tccagatttc gttgtctcag
     accttctgtc tcaagctgaa catggtattg attcccaggt gattctgttg gctgtcgata
     tgacagacaa ggagcttgcc agaattgaag atgctgttca caaccaagct gtgcagttgc
     caagggttga aattgtacgc aagtgtattg cacactctac aaccctatcg gttgcaacct
     acgagcaggc tttggaaatg tccaatcagt acgctcctga acacttgatc ctgcaaatcg
     agaatgcttc ttatgttgat caagtacaac acgctggatc tgtgtttgtt ggtgcctact
     ctccagagag ttgtggagat tactcctccg gtaccaacca cactttgcca acgtacggat
     atgcccgtca atacagcgga gttaacactg caaccttcca gaagttcatc acttcacaag
     acgtaactcc tgagggactg aaacatattg gccaagcagt gatggatctg gctgctgttg
     aaggtctaga tgctcaccgc aatgctgtta aggttcgtat ggagaaactg ggacttattt
     aattatttag agattttaac ttacatttag attcgataga tcattattga agcatttatc
     agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//