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ID   PSG931     preliminary; circular DNA; SYN; 5535 BP.
XX
AC   U14117;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG931 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5535
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5535
RC   pSG931 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537456
CC   NM (pSG931)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG931 5535bp"
FT   CDS             complement(1888..2748)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2848..5376
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5535 BP; 1477 A; 1263 C; 1288 G; 1507 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg ggggcgcgcc
     ggatccttaa ttaagtctag agtcgactgt ttaaacctgc aggcatgcaa gcttggcgta
     atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     tcaatgatct cctgatgact gactcactga taataaaaat acggcttcag aatttctcaa
     gactacactc actgtccgac ttcaagtatg acatttccct tgctacctgc atacgcaagt
     gttgcagagt ttgataattc cttgagtttg gtaggaaaag ccgtgtttcc ctatgctgct
     gaccagctgc acaacctgat caagttcact caatcgactg agcttcaagt taatgtgcaa
     gttgagtcat ccgttacaga ggaccaattt gaggagctga tcgacaactt gctcaagttg
     tacaataatg gtatcaatga agtgattttg gacctagatt tggcagaaag agttgtccaa
     aggatcccag gcgctagggt tatctatagg accctggttg ataaagttgc atccttgccc
     gctaatgcta gtatcgctgt gcctttttct tctccactgg gcgatttgaa aagtttcact
     aatggcggta gtagaactgt ttatgctttt tctgagaccg caaagttggt agatgtgact
     tccactgttg cttctggtat aatccccatt attgatgctc ggcaattgac tactgaatac
     gaactttctg aagatgtcaa aaagttccct gtcagtgaaa ttttgttggc gtctttgact
     actgaccgcc ccgatggtct attcactact ttggtggctg actcttctaa ttactcgttg
     ggcctggtgt actcgtccaa aaagtctatt ccggaggcta taaggacaca aactggagtc
     taccaatctc gtcgtcacgg tttgtggtat aaaggtgcta catctggagc aactcaaaag
     ttgctgggta tcgaattgga ttgtgatgga gactgcttga aatttgtggt tgaacaaaca
     ggtgttggtt tctgtcactt ggaacgcact tcctgttttg gccaatcaaa gggtcttaga
     gccatggaag ccaccttgtg ggatcgtaag agcaatgctc cagaaggttc ttataccaaa
     cggttatttg acgacgaagt tttgttgaac gctaaaatta gggaggaagc tgatgaactt
     gcagaagcta aatccaagga agatatagcc tgggaatgtg ctgacttatt ttattttgca
     ttagttagat gtgccaagta cggcgtgacg ttggacgagg tggagagaaa cctggatatg
     aagtccctaa aggtcactag aaggaaagga gatgccaagc caggatacac caaggaacaa
     cctaaagaag aatccaaacc taaagaagtc ccttctgaag gtcgtattga attgtgcaaa
     attgacgttt ctaaggcctc ctcacaagaa attgaagatg cccttcgtcg tcctatccag
     aaaacggaac agattatgga attagtcaaa ccaattgtcg acaatgttcg tcaaaatggt
     gacaaagccc ttttagaact aactgccaag tttgatggag tcgctttgaa gacacctgtg
     ttagaagctc ctttcccaga ggaacttatg caattgccag ataacgttaa gagagccatt
     gatctctcta tagataacgt caggaaattc catgaagctc aactagcgga gacgttgcaa
     gttgagactt gccctggtgt agtctgctct cgttttgcaa gacctattga gaaagttggc
     ctctatattc ctggtggaac cgcaattctg ccttccactt ccctgatgct gggtgttcct
     gccaaagttg ctggttgcaa agaaattgtt tttgcatctc cacctaagaa ggatggtacc
     cttaccccag aagtcatcta cgttgcccac aaggttggtg ctaagtgtat cgtgctagca
     ggaggcgccc aggcagtagc tgctatggct tacggaacag aaactgttcc taagtgtgac
     aaaatatttg gtccaggaaa ccagttcgtt actgctgcca agatgatggt tcaaaatgac
     acatcagccc tgtgtagtat tgacatgcct gctgggcctt ctgaagttct agttattgct
     gataaatacg ctgatccaga tttcgttgtc tcagaccttc tgtctcaagc tgaacatggt
     attgattccc aggtgattct gttggctgtc gatatgacag acaaggagct tgccagaatt
     gaagatgctg ttcacaacca agctgtgcag ttgccaaggg ttgaaattgt acgcaagtgt
     attgcacact ctacaaccct atcggttgca acctacgagc aggctttgga aatgtccaat
     cagtacgctc ctgaacactt gatcctgcaa atcgagaatg cttcttatgt tgatcaagta
     caacacgctg gatctgtgtt tgttggtgcc tactctccag agagttgtgg agattactcc
     tccggtacca accacacttt gccaacgtac ggatatgccc gtcaatacag cggagttaac
     actgcaacct tccagaagtt catcacttca caagacgtaa ctcctgaggg actgaaacat
     attggccaag cagtgatgga tctggctgct gttgaaggtc tagatgctca ccgcaatgct
     gttaaggttc gtatggagaa actgggactt atttaattat ttagagattt taacttacat
     ttagattcga tagatcatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     gtgccacctg acgtc
//