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ID   PSG932     preliminary; circular DNA; SYN; 5936 BP.
XX
AC   U13762;
AC   U14116;
XX
DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli vector pSG932 - complete.
XX
KW   cloning vector; HIS4 gene.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5936
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
XX
RN   [2]
RP   1-5936
RC   pSG932
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
XX
CC   NCBI gi: 537453
CC   NM (pSG932)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG932 5936bp"
FT   CDS             complement(2289..3149)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             3249..5777
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5936 BP; 1568 A; 1365 C; 1387 G; 1616 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     ttggagcttg gcgtaatcat ggtcattttt tcgtccgatt aggcaagctt aaggtgcacg
     gcccacgtgg ccactagtac ttctcgagct ctgtagatgt ccgcggtcgc gacgtacgcg
     tatcgatggc gccagctgca ggcggccgcc atatgcatcc taggcctatt aatattccgg
     agtatacgta gccggctaac gttaacaacc ggtacctcta gaactatagc tagcatgcgc
     aaatttaaag cgctgatatc gatcgcgcgc agatctgtca tgatgatcat tgcaattgga
     tccatatata gggcccgggt tataattacc tcaggtcgac gtcccatgtg caggtgctga
     attcgaaggc attccacgac gtggtcacca gccatggctc cagcctgtcc tcgtaccagc
     ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatgatc tcctgatgac
     tgactcactg ataataaaaa tacggcttca gaatttctca agactacact cactgtccga
     cttcaagtat gacatttccc ttgctacctg catacgcaag tgttgcagag tttgataatt
     ccttgagttt ggtaggaaaa gccgtgtttc cctatgctgc tgaccagctg cacaacctga
     tcaagttcac tcaatcgact gagcttcaag ttaatgtgca agttgagtca tccgttacag
     aggaccaatt tgaggagctg atcgacaact tgctcaagtt gtacaataat ggtatcaatg
     aagtgatttt ggacctagat ttggcagaaa gagttgtcca aaggatccca ggcgctaggg
     ttatctatag gaccctggtt gataaagttg catccttgcc cgctaatgct agtatcgctg
     tgcctttttc ttctccactg ggcgatttga aaagtttcac taatggcggt agtagaactg
     tttatgcttt ttctgagacc gcaaagttgg tagatgtgac ttccactgtt gcttctggta
     taatccccat tattgatgct cggcaattga ctactgaata cgaactttct gaagatgtca
     aaaagttccc tgtcagtgaa attttgttgg cgtctttgac tactgaccgc cccgatggtc
     tattcactac tttggtggct gactcttcta attactcgtt gggcctggtg tactcgtcca
     aaaagtctat tccggaggct ataaggacac aaactggagt ctaccaatct cgtcgtcacg
     gtttgtggta taaaggtgct acatctggag caactcaaaa gttgctgggt atcgaattgg
     attgtgatgg agactgcttg aaatttgtgg ttgaacaaac aggtgttggt ttctgtcact
     tggaacgcac ttcctgtttt ggccaatcaa agggtcttag agccatggaa gccaccttgt
     gggatcgtaa gagcaatgct ccagaaggtt cttataccaa acggttattt gacgacgaag
     ttttgttgaa cgctaaaatt agggaggaag ctgatgaact tgcagaagct aaatccaagg
     aagatatagc ctgggaatgt gctgacttat tttattttgc attagttaga tgtgccaagt
     acggcgtgac gttggacgag gtggagagaa acctggatat gaagtcccta aaggtcacta
     gaaggaaagg agatgccaag ccaggataca ccaaggaaca acctaaagaa gaatccaaac
     ctaaagaagt cccttctgaa ggtcgtattg aattgtgcaa aattgacgtt tctaaggcct
     cctcacaaga aattgaagat gcccttcgtc gtcctatcca gaaaacggaa cagattatgg
     aattagtcaa accaattgtc gacaatgttc gtcaaaatgg tgacaaagcc cttttagaac
     taactgccaa gtttgatgga gtcgctttga agacacctgt gttagaagct cctttcccag
     aggaacttat gcaattgcca gataacgtta agagagccat tgatctctct atagataacg
     tcaggaaatt ccatgaagct caactagcgg agacgttgca agttgagact tgccctggtg
     tagtctgctc tcgttttgca agacctattg agaaagttgg cctctatatt cctggtggaa
     ccgcaattct gccttccact tccctgatgc tgggtgttcc tgccaaagtt gctggttgca
     aagaaattgt ttttgcatct ccacctaaga aggatggtac ccttacccca gaagtcatct
     acgttgccca caaggttggt gctaagtgta tcgtgctagc aggaggcgcc caggcagtag
     ctgctatggc ttacggaaca gaaactgttc ctaagtgtga caaaatattt ggtccaggaa
     accagttcgt tactgctgcc aagatgatgg ttcaaaatga cacatcagcc ctgtgtagta
     ttgacatgcc tgctgggcct tctgaagttc tagttattgc tgataaatac gctgatccag
     atttcgttgt ctcagacctt ctgtctcaag ctgaacatgg tattgattcc caggtgattc
     tgttggctgt cgatatgaca gacaaggagc ttgccagaat tgaagatgct gttcacaacc
     aagctgtgca gttgccaagg gttgaaattg tacgcaagtg tattgcacac tctacaaccc
     tatcggttgc aacctacgag caggctttgg aaatgtccaa tcagtacgct cctgaacact
     tgatcctgca aatcgagaat gcttcttatg ttgatcaagt acaacacgct ggatctgtgt
     ttgttggtgc ctactctcca gagagttgtg gagattactc ctccggtacc aaccacactt
     tgccaacgta cggatatgcc cgtcaataca gcggagttaa cactgcaacc ttccagaagt
     tcatcacttc acaagacgta actcctgagg gactgaaaca tattggccaa gcagtgatgg
     atctggctgc tgttgaaggt ctagatgctc accgcaatgc tgttaaggtt cgtatggaga
     aactgggact tatttaatta tttagagatt ttaacttaca tttagattcg atagatcatt
     attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//