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ID   PSG933     preliminary; circular DNA; SYN; 5239 BP.
XX
AC   U14124;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG933 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5239
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5239
RC   pSG933
RA   Gould S.J.;
RT   "Direct Submission";
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537477
CC   NM (pSG933)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG933 5239bp"
FT   CDS             complement(1592..2452)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2552..5080
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5239 BP; 1419 A; 1182 C; 1187 G; 1451 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
     atacgattta ggtgacacta tagaactcga gcagctgaag cttgaattca tcgatgatat
     cagatctgcc ggtctcccta tagtgagtcg tattaatttc gataagccag gttaacctgc
     attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     tttttcaatg atctcctgat gactgactca ctgataataa aaatacggct tcagaatttc
     tcaagactac actcactgtc cgacttcaag tatgacattt cccttgctac ctgcatacgc
     aagtgttgca gagtttgata attccttgag tttggtagga aaagccgtgt ttccctatgc
     tgctgaccag ctgcacaacc tgatcaagtt cactcaatcg actgagcttc aagttaatgt
     gcaagttgag tcatccgtta cagaggacca atttgaggag ctgatcgaca acttgctcaa
     gttgtacaat aatggtatca atgaagtgat tttggaccta gatttggcag aaagagttgt
     ccaaaggatc ccaggcgcta gggttatcta taggaccctg gttgataaag ttgcatcctt
     gcccgctaat gctagtatcg ctgtgccttt ttcttctcca ctgggcgatt tgaaaagttt
     cactaatggc ggtagtagaa ctgtttatgc tttttctgag accgcaaagt tggtagatgt
     gacttccact gttgcttctg gtataatccc cattattgat gctcggcaat tgactactga
     atacgaactt tctgaagatg tcaaaaagtt ccctgtcagt gaaattttgt tggcgtcttt
     gactactgac cgccccgatg gtctattcac tactttggtg gctgactctt ctaattactc
     gttgggcctg gtgtactcgt ccaaaaagtc tattccggag gctataagga cacaaactgg
     agtctaccaa tctcgtcgtc acggtttgtg gtataaaggt gctacatctg gagcaactca
     aaagttgctg ggtatcgaat tggattgtga tggagactgc ttgaaatttg tggttgaaca
     aacaggtgtt ggtttctgtc acttggaacg cacttcctgt tttggccaat caaagggtct
     tagagccatg gaagccacct tgtgggatcg taagagcaat gctccagaag gttcttatac
     caaacggtta tttgacgacg aagttttgtt gaacgctaaa attagggagg aagctgatga
     acttgcagaa gctaaatcca aggaagatat agcctgggaa tgtgctgact tattttattt
     tgcattagtt agatgtgcca agtacggcgt gacgttggac gaggtggaga gaaacctgga
     tatgaagtcc ctaaaggtca ctagaaggaa aggagatgcc aagccaggat acaccaagga
     acaacctaaa gaagaatcca aacctaaaga agtcccttct gaaggtcgta ttgaattgtg
     caaaattgac gtttctaagg cctcctcaca agaaattgaa gatgcccttc gtcgtcctat
     ccagaaaacg gaacagatta tggaattagt caaaccaatt gtcgacaatg ttcgtcaaaa
     tggtgacaaa gcccttttag aactaactgc caagtttgat ggagtcgctt tgaagacacc
     tgtgttagaa gctcctttcc cagaggaact tatgcaattg ccagataacg ttaagagagc
     cattgatctc tctatagata acgtcaggaa attccatgaa gctcaactag cggagacgtt
     gcaagttgag acttgccctg gtgtagtctg ctctcgtttt gcaagaccta ttgagaaagt
     tggcctctat attcctggtg gaaccgcaat tctgccttcc acttccctga tgctgggtgt
     tcctgccaaa gttgctggtt gcaaagaaat tgtttttgca tctccaccta agaaggatgg
     tacccttacc ccagaagtca tctacgttgc ccacaaggtt ggtgctaagt gtatcgtgct
     agcaggaggc gcccaggcag tagctgctat ggcttacgga acagaaactg ttcctaagtg
     tgacaaaata tttggtccag gaaaccagtt cgttactgct gccaagatga tggttcaaaa
     tgacacatca gccctgtgta gtattgacat gcctgctggg ccttctgaag ttctagttat
     tgctgataaa tacgctgatc cagatttcgt tgtctcagac cttctgtctc aagctgaaca
     tggtattgat tcccaggtga ttctgttggc tgtcgatatg acagacaagg agcttgccag
     aattgaagat gctgttcaca accaagctgt gcagttgcca agggttgaaa ttgtacgcaa
     gtgtattgca cactctacaa ccctatcggt tgcaacctac gagcaggctt tggaaatgtc
     caatcagtac gctcctgaac acttgatcct gcaaatcgag aatgcttctt atgttgatca
     agtacaacac gctggatctg tgtttgttgg tgcctactct ccagagagtt gtggagatta
     ctcctccggt accaaccaca ctttgccaac gtacggatat gcccgtcaat acagcggagt
     taacactgca accttccaga agttcatcac ttcacaagac gtaactcctg agggactgaa
     acatattggc caagcagtga tggatctggc tgctgttgaa ggtctagatg ctcaccgcaa
     tgctgttaag gttcgtatgg agaaactggg acttatttaa ttatttagag attttaactt
     acatttagat tcgatagatc attattgaag catttatcag ggttattgtc tcatgagcgg
     atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     aaaagtgcca cctgacgtc
//