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ID   PSG934     preliminary; circular DNA; SYN; 5284 BP.
XX
AC   U14123;
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DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG934 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5284
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
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RN   [2]
RP   1-5284
RC   pSG934
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
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CC   NCBI gi: 537474
CC   NM (pSG934)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG934 5284bp"
FT   CDS             complement(1637..2497)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   CDS             2597..5125
FT                   /note="GEN histidinol dehydrogenase gene"
XX
SQ   Sequence 5284 BP; 1427 A; 1197 C; 1201 G; 1459 T; 0 other;
     cgaccaatta catacccctc atagaactac gttggaacat agtccgtccc cgggggaaga
     tttattgtct caaaaggtca atttcatatt ttatatgcat tcaatactta tttattatta
     atttagcttg actacgatgc atataatttt aattttattt taaattatat atgaggtaag
     agtataactc taaacctaat aaatatataa ttaattatac gcaatagtta aaccatagat
     taattacaac taatcctttc gtactaagtt gtaatccttt attgacattt ccctaaagca
     gatagaaacc atactgtctc acgactatta aacccaactc acgtaacctt ttaattgacg
     aacagtcaaa cccttatcag cgtgtgctac caataggata ggttgagtcg acatcgacat
     atggacatat tgtcgttaga acgcggctac aattaataca taaccttatg tatcatacac
     atacgattta ggtgacacta tagaactcga gcagctgaag cttgcatgcc tgcaggtcga
     ctctagagga tccccgggta ccgagctcga attcatcgat gatatcagat ctgccggtct
     ccctatagtg agtcgtatta atttcgataa gccaggttaa cctgcattaa tgaatcggcc
     aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatgatctc
     ctgatgactg actcactgat aataaaaata cggcttcaga atttctcaag actacactca
     ctgtccgact tcaagtatga catttccctt gctacctgca tacgcaagtg ttgcagagtt
     tgataattcc ttgagtttgg taggaaaagc cgtgtttccc tatgctgctg accagctgca
     caacctgatc aagttcactc aatcgactga gcttcaagtt aatgtgcaag ttgagtcatc
     cgttacagag gaccaatttg aggagctgat cgacaacttg ctcaagttgt acaataatgg
     tatcaatgaa gtgattttgg acctagattt ggcagaaaga gttgtccaaa ggatcccagg
     cgctagggtt atctatagga ccctggttga taaagttgca tccttgcccg ctaatgctag
     tatcgctgtg cctttttctt ctccactggg cgatttgaaa agtttcacta atggcggtag
     tagaactgtt tatgcttttt ctgagaccgc aaagttggta gatgtgactt ccactgttgc
     ttctggtata atccccatta ttgatgctcg gcaattgact actgaatacg aactttctga
     agatgtcaaa aagttccctg tcagtgaaat tttgttggcg tctttgacta ctgaccgccc
     cgatggtcta ttcactactt tggtggctga ctcttctaat tactcgttgg gcctggtgta
     ctcgtccaaa aagtctattc cggaggctat aaggacacaa actggagtct accaatctcg
     tcgtcacggt ttgtggtata aaggtgctac atctggagca actcaaaagt tgctgggtat
     cgaattggat tgtgatggag actgcttgaa atttgtggtt gaacaaacag gtgttggttt
     ctgtcacttg gaacgcactt cctgttttgg ccaatcaaag ggtcttagag ccatggaagc
     caccttgtgg gatcgtaaga gcaatgctcc agaaggttct tataccaaac ggttatttga
     cgacgaagtt ttgttgaacg ctaaaattag ggaggaagct gatgaacttg cagaagctaa
     atccaaggaa gatatagcct gggaatgtgc tgacttattt tattttgcat tagttagatg
     tgccaagtac ggcgtgacgt tggacgaggt ggagagaaac ctggatatga agtccctaaa
     ggtcactaga aggaaaggag atgccaagcc aggatacacc aaggaacaac ctaaagaaga
     atccaaacct aaagaagtcc cttctgaagg tcgtattgaa ttgtgcaaaa ttgacgtttc
     taaggcctcc tcacaagaaa ttgaagatgc ccttcgtcgt cctatccaga aaacggaaca
     gattatggaa ttagtcaaac caattgtcga caatgttcgt caaaatggtg acaaagccct
     tttagaacta actgccaagt ttgatggagt cgctttgaag acacctgtgt tagaagctcc
     tttcccagag gaacttatgc aattgccaga taacgttaag agagccattg atctctctat
     agataacgtc aggaaattcc atgaagctca actagcggag acgttgcaag ttgagacttg
     ccctggtgta gtctgctctc gttttgcaag acctattgag aaagttggcc tctatattcc
     tggtggaacc gcaattctgc cttccacttc cctgatgctg ggtgttcctg ccaaagttgc
     tggttgcaaa gaaattgttt ttgcatctcc acctaagaag gatggtaccc ttaccccaga
     agtcatctac gttgcccaca aggttggtgc taagtgtatc gtgctagcag gaggcgccca
     ggcagtagct gctatggctt acggaacaga aactgttcct aagtgtgaca aaatatttgg
     tccaggaaac cagttcgtta ctgctgccaa gatgatggtt caaaatgaca catcagccct
     gtgtagtatt gacatgcctg ctgggccttc tgaagttcta gttattgctg ataaatacgc
     tgatccagat ttcgttgtct cagaccttct gtctcaagct gaacatggta ttgattccca
     ggtgattctg ttggctgtcg atatgacaga caaggagctt gccagaattg aagatgctgt
     tcacaaccaa gctgtgcagt tgccaagggt tgaaattgta cgcaagtgta ttgcacactc
     tacaacccta tcggttgcaa cctacgagca ggctttggaa atgtccaatc agtacgctcc
     tgaacacttg atcctgcaaa tcgagaatgc ttcttatgtt gatcaagtac aacacgctgg
     atctgtgttt gttggtgcct actctccaga gagttgtgga gattactcct ccggtaccaa
     ccacactttg ccaacgtacg gatatgcccg tcaatacagc ggagttaaca ctgcaacctt
     ccagaagttc atcacttcac aagacgtaac tcctgaggga ctgaaacata ttggccaagc
     agtgatggat ctggctgctg ttgaaggtct agatgctcac cgcaatgctg ttaaggttcg
     tatggagaaa ctgggactta tttaattatt tagagatttt aacttacatt tagattcgat
     agatcattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     cgtc
//