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ID   PSG935     preliminary; circular DNA; SYN; 5639 BP.
XX
AC   U14122;
XX
DT   13-SEP-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli vector pSG935 - complete.
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KW   cloning vector; HIS4 gene.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5639
RC   pSG925, pSG926, pSG927, pSG928, pSG929 from Pichia pastoris HIS4 gene
RC   pSG930, pSG931, pSG932, pSG933, pSG934 from Pichia pastoris HIS4 gene
RC   pSG935 from Pichia pastoris HIS4 gene
RA   Gould S.J.;
RT   "The Pichia pastoris HIS4 gene: nucleotide sequence, creation of a
RT   non-reverting his4 deletion mutant, and development of HIS4-based
RT   replicating and integrating plasmids";
RL   Curr. Genet. 26:0-0(1994).
XX
RN   [2]
RP   1-5639
RC   pSG935
RA   Gould S.J.;
RT   ;
RL   Submitted (29-AUG-1994) by:
RL   Gould S.J., Neurogenetics, The Kennedy Krieger Institute,
RL   707 North Broadway, Baltimore, MD 21030, USA.
XX
CC   NCBI gi: 537471
CC   NM (pSG935)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. Pichia pastoris, HIS4 gene
FT                   -> pSG935 5639bp"
FT   CDS             833..3361
FT                   /note="GEN histidinol dehydrogenase gene"
FT   CDS             complement(4651..5511)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
XX
SQ   Sequence 5639 BP; 1473 A; 1310 C; 1372 G; 1484 T; 0 other;
     cgaattacat acccctcata gaactacgtt gctaaattgt aagcgttaat attttgttaa
     aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
     agcttgatat cgaattcctg cagcccgggg gatctcctga tgactgactc actgataata
     aaaatacggc ttcagaattt ctcaagacta cactcactgt ccgacttcaa gtatgacatt
     tcccttgcta cctgcatacg caagtgttgc agagtttgat aattccttga gtttggtagg
     aaaagccgtg tttccctatg ctgctgacca gctgcacaac ctgatcaagt tcactcaatc
     gactgagctt caagttaatg tgcaagttga gtcatccgtt acagaggacc aatttgagga
     gctgatcgac aacttgctca agttgtacaa taatggtatc aatgaagtga ttttggacct
     agatttggca gaaagagttg tccaaaggat cccaggcgct agggttatct ataggaccct
     ggttgataaa gttgcatcct tgcccgctaa tgctagtatc gctgtgcctt tttcttctcc
     actgggcgat ttgaaaagtt tcactaatgg cggtagtaga actgtttatg ctttttctga
     gaccgcaaag ttggtagatg tgacttccac tgttgcttct ggtataatcc ccattattga
     tgctcggcaa ttgactactg aatacgaact ttctgaagat gtcaaaaagt tccctgtcag
     tgaaattttg ttggcgtctt tgactactga ccgccccgat ggtctattca ctactttggt
     ggctgactct tctaattact cgttgggcct ggtgtactcg tccaaaaagt ctattccgga
     ggctataagg acacaaactg gagtctacca atctcgtcgt cacggtttgt ggtataaagg
     tgctacatct ggagcaactc aaaagttgct gggtatcgaa ttggattgtg atggagactg
     cttgaaattt gtggttgaac aaacaggtgt tggtttctgt cacttggaac gcacttcctg
     ttttggccaa tcaaagggtc ttagagccat ggaagccacc ttgtgggatc gtaagagcaa
     tgctccagaa ggttcttata ccaaacggtt atttgacgac gaagttttgt tgaacgctaa
     aattagggag gaagctgatg aacttgcaga agctaaatcc aaggaagata tagcctggga
     atgtgctgac ttattttatt ttgcattagt tagatgtgcc aagtacggcg tgacgttgga
     cgaggtggag agaaacctgg atatgaagtc cctaaaggtc actagaagga aaggagatgc
     caagccagga tacaccaagg aacaacctaa agaagaatcc aaacctaaag aagtcccttc
     tgaaggtcgt attgaattgt gcaaaattga cgtttctaag gcctcctcac aagaaattga
     agatgccctt cgtcgtccta tccagaaaac ggaacagatt atggaattag tcaaaccaat
     tgtcgacaat gttcgtcaaa atggtgacaa agccctttta gaactaactg ccaagtttga
     tggagtcgct ttgaagacac ctgtgttaga agctcctttc ccagaggaac ttatgcaatt
     gccagataac gttaagagag ccattgatct ctctatagat aacgtcagga aattccatga
     agctcaacta gcggagacgt tgcaagttga gacttgccct ggtgtagtct gctctcgttt
     tgcaagacct attgagaaag ttggcctcta tattcctggt ggaaccgcaa ttctgccttc
     cacttccctg atgctgggtg ttcctgccaa agttgctggt tgcaaagaaa ttgtttttgc
     atctccacct aagaaggatg gtacccttac cccagaagtc atctacgttg cccacaaggt
     tggtgctaag tgtatcgtgc tagcaggagg cgcccaggca gtagctgcta tggcttacgg
     aacagaaact gttcctaagt gtgacaaaat atttggtcca ggaaaccagt tcgttactgc
     tgccaagatg atggttcaaa atgacacatc agccctgtgt agtattgaca tgcctgctgg
     gccttctgaa gttctagtta ttgctgataa atacgctgat ccagatttcg ttgtctcaga
     ccttctgtct caagctgaac atggtattga ttcccaggtg attctgttgg ctgtcgatat
     gacagacaag gagcttgcca gaattgaaga tgctgttcac aaccaagctg tgcagttgcc
     aagggttgaa attgtacgca agtgtattgc acactctaca accctatcgg ttgcaaccta
     cgagcaggct ttggaaatgt ccaatcagta cgctcctgaa cacttgatcc tgcaaatcga
     gaatgcttct tatgttgatc aagtacaaca cgctggatct gtgtttgttg gtgcctactc
     tccagagagt tgtggagatt actcctccgg taccaaccac actttgccaa cgtacggata
     tgcccgtcaa tacagcggag ttaacactgc aaccttccag aagttcatca cttcacaaga
     cgtaactcct gagggactga aacatattgg ccaagcagtg atggatctgg ctgctgttga
     aggtctagat gctcaccgca atgctgttaa ggttcgtatg gagaaactgg gacttattta
     attatttaga gattttaact tacatttaga ttcgatagat ccactagttc tagagcggcc
     gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
     gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//