The pCI and pSI Mammalian Expression Vectors are designed to promote constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the two vectors is the enhancer/promoter region controlling the expression of the inserted gene. The pSI Expression Vector contains the simian virus 40 (SV40) enhancer and early promoter region, whereas the pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region.

 back Return to this vector's summary.



LOCUS       CVU47121     3634 bp    DNA   circular  SYN       20-FEB-1996
DEFINITION  Cloning vector pSI, mammalian expression vector, complete sequence.
ACCESSION   U47121
NID         g1197677
KEYWORDS    .
SOURCE      Cloning vector pSI.
  ORGANISM  Cloning vector pSI
            artificial sequence; cloning vectors.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Brondyk,W.H.
  TITLE     pCI and pSI Mammalian Expression Vectors
  JOURNAL   Promega Notes 49, 7-11 (1994)
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Brondyk,W.H. and Groskreutz,D.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-1996) Melinda Brady, R&D, Promega Corporation,
            5445 East Cheryl Parkway, Madison, WI 53711, USA
COMMENT
            NCBI gi: 1197677
FEATURES               Location/Qualifiers
     source          1..3634
                     /organism="Cloning vector pSI"
     promoter        1..419
                     /note="SV40 enhancer and early promoter"
     intron          483..615
                     /note="chimeric intron"
     promoter        660..678
                     /note="T7 promoter"
     misc_feature    677
                     /note="T7 promoter transcription start site"
     misc_feature    678..730
                     /note="multiple cloning sites"
     polyA_signal    737..958
                     /note="SV40 late polyadenylation signal"
     rep_origin      1048..1503
                     /note="phage f1 region"
     CDS             1942..2802
                     /gene="ampR"
                     /note="NCBI gi: 1197678"
                     /codon_start=1
                     /function="ampicillin resistance"
                     /product="beta-lactamase"
                     /db_xref="PID:g1197678"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT      913 a    894 c    886 g    941 t
ORIGIN      
        1 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
       61 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      421 attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga ggcactgggc
      481 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
      541 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
      601 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt
      661 aatacgactc actataggct agcctcgaga attcacgcgt ggtacctcta gagtcgaccc
      721 gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
      781 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
      841 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
      901 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
      961 atcgataagg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1021 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
     1081 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1141 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     1201 gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc aaaaaacttg
     1261 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     1321 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     1381 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     1441 aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta acgtttacaa
     1501 tttcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     1561 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1621 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1681 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1741 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1801 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1861 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1921 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1981 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2041 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2101 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2161 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2221 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2281 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2341 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2401 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2461 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2521 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2581 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2641 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2701 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2761 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2821 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2881 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2941 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3001 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3061 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3121 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3181 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3241 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3301 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3361 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3421 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3481 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3541 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3601 tgctggcctt ttgctcacat ggctcgacag atct
//