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ID PSK16 preliminary; circular DNA; SYN; 7272 BP.
XX
AC IG5055; S38358;
XX
DT 01-OCT-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pSK16 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pSK15 from pUC18 & lambda
RC pSK16 from M13mp18 & lambda
RC pSK28 from pSK15 & pSU11, bsuRIM gene
RC pSK30 from pSK28 & pES2
RA Kim S., Posfai G., Szybalski W.;
RT "A novel gene-fusing vector: construction of a 5'-GGmCC-specific
RT chimeric methyltransferase, M.BspRI/M.BsuRI";
RL Gene 100:45-50(1991).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC not original sequence.
CC NM (pSK16)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (M13mp18)(lambda)
CC BR (pSK15)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. M13mp18 HincII 7249bp 6266..6266
FT 2. lambda FokI-HinfI 23bp, MspI/HpaII-FokI site
FT \ complement
FT Klenow:Klenow
FT oligo 23bp taacgcccggcttcatccggatt
FT -> pSK16 7272bp [unique BspMI, MspI-FokI]"
FT - 1..6265
FT /note="M13mp18 1..6265 6265bp
FT HindII = HincII = GTY^RAC
FT FokI = GGATG(9/13)
FT \ taacgcccggcttcatccggatt"
FT - 6266..6288
FT /note="taacgcccggcttcatccggatt 23bp
FT \ taacgcccggcttcatccggatt
FT HinfI = G^ANT C
FT HindII = HincII = GTY^RAC"
FT - 6289..7272
FT /note="M13mp18 6266..7249 984bp"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ Sequence 7272 BP; 1772 A; 1546 C; 1538 G; 2416 T; 0 other;
aatgctacta ctattagtag aattgatgcc accttttcag ctcgcgcccc aaatgaaaat
atagctaaac aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact
cgttcgcaga attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta
gttgcatatt taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca
tccgcaaaaa tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg
ttggagtttg cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag
tctttcgggc ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt
cagggtaaag acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca
tttgaggggg attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct
aaacatttta ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt
ggtttttatc gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt
aattcctttt ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg
atgaatcttt ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt
tcttcccaac gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca
caatgattaa agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttc
tcgtcagggc aagccttatt cactgaatga gcagctttgt tacgttgatt tgggtaatga
atatccggtt cttgtcaaga ttactcttga tgaaggtcag ccagcctatg cgcctggtct
gtacaccgtt catctgtcct ctttcaaagt tggtcagttc ggttccctta tgattgaccg
tctgcgcctc gttccggcta agtaacatgg agcaggtcgc ggatttcgac acaatttatc
aggcgatgat acaaatctcc gttgtacttt gtttcgcgct tggtataatc gctgggggtc
aaagatgagt gttttagtgt attctttcgc ctctttcgtt ttaggttggt gccttcgtag
tggcattacg tattttaccc gtttaatgga aacttcctca tgaaaaagtc tttagtcctc
aaagcctctg tagccgttgc taccctcgtt ccgatgctgt ctttcgctgc tgagggtgac
gatcccgcaa aagcggcctt taactccctg caagcctcag cgaccgaata tatcggttat
gcgtgggcga tggttgttgt cattgtcggc gcaactatcg gtatcaagct gtttaagaaa
ttcacctcga aagcaagctg ataaaccgat acaattaaag gctccttttg gagccttttt
ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct
attctcactc cgctgaaact gttgaaagtt gtttagcaaa accccataca gaaaattcat
ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggttgtc
tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat
gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt
ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta
ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa
accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc
agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc
aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt
atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgaag
atccattcgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg
ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg
gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg
attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg
aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg
ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg
gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt
taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt
ttgtctttag cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat
tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt
ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt
attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct
taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg
gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt
tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct
ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga
ttgggataaa taatatggct gtttattttg taactggcaa attaggctct ggaaagacgc
tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc
ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc
ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc ggtaatgatt
cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact tggtttaata
cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta catgctcgta
aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat aaacaggcgc
gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt actttacctt
ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct aaattacatg
ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt tggctttata
ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt aattatgatt
ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc aaaccattaa
atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt
gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa cctaagccgg
aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt gactcttctc
agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata
gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt actgtttcca
ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt
gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct gcgcgatttt
gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga tgtaaaaggt
actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt ctttatttct
gttttacgtg ctaataattt tgatatggtt ggttcaattc cttccataat tcagaagtat
aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca ggaatatgat
gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaact
tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt gtttgtaaag
tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct attagttgtt
agtgcaccta aagatatttt agataacctt cctcaattcc tttctactgt tgatttgcca
actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga tgctttagat
ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa tactgaccgc
ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg cgatgtttta
gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgccacgt
attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt cccttttatt
actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac gattgagcgt
caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt
ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt
actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac tcttttactc
ggtggcctca ctgattataa aaacacttct caagattctg gcgtaccgtt cctgtctaaa
atccctttaa tcggcctcct gtttagctcc cgctctgatt ccaacgagga aagcacgtta
tacgtgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
caggcggtga agggcaatca gctgttgccc gtctcgctgg tgaaaagaaa aaccaccctg
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattcgagct
cggtacccgg ggatcctcta gagtctaacg cccggcttca tccggattga cctgcaggca
tgcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg
gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga
tacggtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa
cgtaacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg
ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat
ttttgatggc gttcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
tttaacaaaa tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg
gggcttttct gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg
ttcatcgatt ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagat
ctctcaaaaa tagctaccct ctccggcatt aatttatcag ctagaacggt tgaatatcat
attgatggtg atttgactgt ctccggcctt tctcaccctt ttgaatcttt acctacacat
tactcaggca ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa
ataaaggctt ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta
gctttatgct ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat
ttattggatg tt
//