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ID PSK241 preliminary; circular DNA; SYN; 3830 BP.
XX
AC IG5024;
XX
DT 01-OCT-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pSK241 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pSK222 from pKK223-3 & pGEM-5Zf-
RC pSK241 from pSK222
RA Kunapuli S.P., Colman R.W.;
RT "Two new phagemid vectors for site-directed mutagenesis and
RT expression in E. coli";
RL Biotechniques 14:332-338(1993).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC NM (pSK241)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pSK222 from pKK223-3 and pGEM5Zf-)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEM-5Zf- SfaNI-SfaNI 733bp 2368..3003..98,
FT \ phage f1 ori
FT \ SfaNI = 98 600 1652 1843 2092 2328 2368
FT Klenow:
FT :SphI linker
FT 2. pKK223-3 PvuII-SphI 3084bp 1948..4584..448
FT -> pSK222 3817bp
FT 1. pSK222 remove PstI-EcoRI 18bp 4565..4583, 3799bp
FT \ pGEM5Zf- PstI 74
FT 2. oligo 38bp aattcgagctcgagggtacccgggacatctagactgca
FT -> pSK241 3837bp"
FT - 1..2617
FT /note="pKK223-3 1948..4564 2617bp
FT PstI = CTGCA^G
FT \ gtcta..."
FT - 2618..2647
FT /note="gtctagatgtcccgggtaccctcgagctcg 30bp
FT \ ...gctcg
FT EcoRI = G^AATTC"
FT - 2648..3096
FT /note="pKK223-3 4583..4584..447 449bp
FT SphI = GCATG^C
FT \ c"
FT - 3097..3097
FT /note="c 1bp
FT \ c
FT SphI = GCATG^C
FT SfaNI = GCATC(5/9)aggcg
FT \ acgc..."
FT - 3098..3830
FT /note="pGEM-5Zf- 2368..3003..97 733bp
FT \ ctccc
FT SfaNI = aacgcgttggatgc
FT SfaNI = GCATC(5/9)
FT PvuII = CAG^CTG"
FT misc_binding 0..0
FT /note="SIT BamHI"
FT promoter 0..0
FT /note="PRO E. coli tac"
FT misc_binding 1..38
FT /note="MCS unique EcoRI-XhoI-SacI-KpnI-SmaI-BglII-
FT XbaI-PstI-HindIII"
FT misc_binding 0..0
FT /note="SIT XmnI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT rep_origin 0..0
FT /note="ORI phage f1"
XX
SQ Sequence 3830 BP; 891 A; 1011 C; 975 G; 953 T; 0 other;
ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacagcatc gtggtgtcac
gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
tttagaaaaa taaacaaaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg
gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg
caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca ggctgaaaat
cttctctcat ccgccaaaac agccaagctt ggctgcagtc tagatgtccc gggtaccctc
gagctcgaat tctgtttcct gtgtgaaatt gttatccgct cacaattcca cacattatac
gagccgatga ttaattgtca acagctcatt tcagaatatt tgccagaacc gttatgatgt
cggcgcaaaa aacattatcc agaacgggag tgcgccttga gcgacacgaa ttatgcagtg
atttacgacc tgcacagcca taccacagct tccgatggct gcctgacgcc agaagcattg
gtgcaccgtg cagtcgataa gcccggatcc tctacgccgg acgcatcgtg gccggcatca
ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
tggccggggg actgttgggc gccatctcct tgcatgcacg cgccctgtag cggcgcatta
agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
gctctaaatc gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc
aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
tattggttaa aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta
acgtttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
acgactcact atagggcgaa ttgggcccga cgtcgcatgc tcccggccgc catggccgcg
ggatatcact agtgcggccg cctgcaggtc gaccatatgg gagagctccc
//