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ID   PSKS101    preliminary; circular DNA; SYN; 3597 BP.
XX
AC   IG9941;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSKS101 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSKS101)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN ()
CC   SE ()
CC   PA (pUC7)(Tn5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC7 PstI 2674bp 256..256
FT                   2. Tn5 PstI-PstI 923bp 1732..2655, kan/neo gene
FT                   -> pSKS101 3597bp"
FT   -               1..255
FT                   /note="pUC7 1..255 255bp
FT                   PstI = CTGCA^G"
FT   -               256..1178
FT                   /note="Tn5 1732..2654 923bp
FT                   PstI = CTGCA^G"
FT   -               1179..3597
FT                   /note="pUC7 256..2674 2419bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase lacY gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase lacA gene"
XX
SQ   Sequence 3597 BP; 822 A; 962 C; 954 G; 859 T; 0 other;
     gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattccccgg
     atccgtcgac ctgcaaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     gctggagttc ttcgcccacc ccgggctcga tcccctcgcg agttggttca gctgctgcct
     gaggctggac gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca tccaggaaac
     cagcagcggc tatccgcgca tccatgcccc cgaactgcgg tcgacggatc cggggaattc
     actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaga
//