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ID   PSKS103    preliminary; circular DNA; SYN; 4462 BP.
XX
AC   IG9800;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Vertebrate/E.coli plasmid vector pSKS103 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSKS103 from pBR322 & pTK2
RC   pSKS108 from pSKS105 & pSKS103
RC   pSKS116 from pSKS108
RC   pSKS111 from pSKS116 & pSKS106, lacZgene
RC   pMC1033 from Tn3 tnpR gene
RC   pSKS121 from pSKS116 & pMC1033
RC   pSKS114 from pSKS116 & Tn9 IS1 & pTK2
RC   pSKS122 from pSKS116 & pSKS114
RA   Shapira S.K., Casadaban M.J.;
RT   "Hybrid protein thymidine kinase gene fusions: plasmid vectors for
RT   the study of transcription and translation initiation signals";
RL   Gene 52:83-94(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSKS103)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(HSV-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. HSV-1 BamHI-BamHI 3579bp 45056..48635,
FT                   \ Q frag tk gene/.28 to .32
FT                   \ [tk gene CDS = #X14112 46672..47802]
FT                   -> pTK2 7940bp [7800bp]
FT                   1. pBR322 remove BamHI-PvuII 1691bp 376..2067, 2670bp
FT                   2. pTK2 PvuII-BglII 1788bp 46068..47856, HSV-1 tk gene
FT                   -> pSKS103 4458bp [amp gene]"
FT   -               1..375
FT                   /note="pBR322 1..375 375bp
FT                   BamHI = G^GATCC
FT                   BglII = A^GATCT"
FT   -               376..2167
FT                   /note="HSV-1 46068..47859 1792bp complement
FT                   PvuII = CAG^CTG"
FT   -               2168..4462
FT                   /note="pBR322 2067..4361 2295bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI"
FT   CDS             0..0
FT                   /note="GEN vertebrate thymidine kinase gene (TK);
FT                   truncated"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4462 BP; 984 A; 1285 C; 1196 G; 997 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgcctt
     gtagaagcgc gtatggcttc gtacccctgc catcaacacg cgtctgcgtt cgaccaggct
     gcgcgttctc gcggccataa caaccgacgt acggcgttgc gccctcgccg gcaacaaaaa
     gccacggaag tccgcctgga gcagaaaatg cccacgctac tgcgggttta tatagacggt
     ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg ttcgcgcgac
     gatatcgtct acgtacccga gccgatgact tactggcggg tgttgggggc ttccgagaca
     atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc ggccggggac
     gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc cgtgaccgac
     gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc gcccccggcc
     ctcaccctca tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc ggccgcgcga
     taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct catcccgccg
     accttgcccg gcacaaacat cgtgttgggg gcccttccgg aggacagaca catcgaccgc
     ctggccaaac gccagcgccc cggcgagcgg cttgacctgg ctatgctggc cgcgattcgc
     cgcgtttatg ggctgcttgc caatacggtg cggtatctgc agggcggcgg gtcgtggcgg
     gaggattggg gacagctttc gggggcggcc gtgccgcccc agggtgccga gccccagagc
     aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg ggcccccgag
     ttgctggccc ccaacggcga cctgtataac gtgtttgcct gggctttgga cgtcttggcc
     aaacgcctcc gtcccatgca tgtctttatc ctggattacg accaatcgcc cgccggctgc
     cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac caccccaggc
     tccataccga cgatctgcga cctggcgcgc acgtttgccc gggagatggg ggaggctaac
     tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     ggcactctgt cgatacccca ccgagacccc attgggacca atacgcccgc gtttcttcct
     tttccccacc ccaaccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     cggcaagccc tgccatagcc acgggccccg tgggttaggg acggggtccc ccatggggaa
     tggtttatgg ttcgtggggg ttattatttt gggcgttgcg tggggtcagg tccacgactg
     gactgagcag acagacccat ggtttttgga tggcctgggc atggaccgca tgtactggcg
     cgacacgaac accgggcgtc tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg
     cggatttctg gcgccgccgg acgaactaaa cctgactacg gcatctctgc cccttcttcg
     ctggtacgag gagcgctttt gttttgtatt ggtcaccacg gccgagtttc cgcgggaccc
     cggccagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     gaggcccttt cgtcttcaag aa
//