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ID   PSKS104    preliminary; circular DNA; SYN; 10130 BP.
XX
AC   K01455;
XX
DT   10-DEC-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSKS104 - complete, lacZ fragment.
XX
KW   cloning vector; ampicillin resistance; beta-galactosidase;
KW   drug resistance gene;
KW   galactosidase; lac operon; lacZ gene; promoter region.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-69
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
RN   [2]
RC   pMC1513 from pMC1403 & pKB252, lacUV5 promoter
RC   pSKS104 from pMC1403 & M13mp7
RC   pSKS105 from pMC1403 & M13mp8
RC   pSKS106 from pMC1403 & M13mp9
RC   pMC1585 from pMC1403 & yeast 2 micron & URA3 gene
RC   pMC1587 from pMC1403 & yeast 2 micron & LEU2 gene
RC   pMC1790 from pMC1403 & ARS1 & TRP1 gene
RC   pMC2010 from pMC1403 & yeast 2 micron & TRP1 gene
RC   pMC931 from pMC1403 & pACYC177
RC   pMC1871 from pMC1403 & M13mp7
RC   [pMC1364 from pMC series]
RC   [pMC1396 from pMC series]
RC   [pMC1511 from pMC series]
RC   [pMC1571 from pMC1511]
RC   [pMC1981 from pMC1871]
RA   Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J.;
RT   "Beta-galactosidase gene fusions for analyzing gene expression in
RT   Escherichia coli and yeast";
RL   Meth. Enzymol. 100:293-308(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Plasmid vector pMC1403 has unique EcoRI, SmaI, and BamHI sites
CC   within the first 24 bp of the beta-galactosidase gene, lacZ.
CC   Plasmid pUC7 is a deleted pBR322 plasmid which is ampicillin
CC   resistant and contains the HaeII lac'IPOZ' segments from M13mp7.
CC   pUC7 has polyrestriction insertions in the fifth and sixth codons
CC   of lacZ which are in phase and do not abolish beta-galactosidase
CC   activity. A 265 bp PvuII lac segment from this plasmid was
CC   incorporated into plasmid pMC1403 by DNA cloning and homologous
CC   recombination within lacZ to form plasmid pSKS104.
CC   NCBI gi: 209310
CC   NM (pSKS104)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC7)(pMC1403)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMC1403 SmaI 9820bp 3647..3647, MCS
FT                   2. pUC7 PvuII-PvuII 310bp 55..365,
FT                   \ lac promoter [265bp]
FT                   -> pSKS104 10130bp"
FT   -               1..3646
FT                   /note="pMC1403 1..3646 3646bp
FT                   SmaI = XmaI = CCC^GGG
FT                   PvuII =       CAG^CTG"
FT   -               3647..3956
FT                   /note="pUC7 55..364 310bp
FT                   PvuII =       CAG^CTG
FT                   SmaI = XmaI = CCC^GGG"
FT   -               3957..10130
FT                   /note="pMC1403 3647..9820 6174bp"
FT   CDS             1..>69
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 209311"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
XX
SQ   Sequence 10130 BP; 2372 A; 2717 C; 2645 G; 2396 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcccctgg cacgacaggt
     ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     gataacaatt tcacacagga aacagctatg accatgatta cgaattcccc ggatccgtcg
     acctgcaggt cgacggatcc ggggaattca ctggccgtcg ttttacaacg tcgtgactgg
     gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccaggggg
     atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
     aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
     cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca
     ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
     ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta
     actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt
     tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga
     tggtgctgcg ttggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg
     gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg
     ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt
     gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg
     tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg
     atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga
     atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag
     cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca
     agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca
     tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg
     tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc
     tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga
     ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg
     atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta
     atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg
     aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg
     atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac
     ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg
     gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg
     tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg
     cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg
     tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt
     tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata
     gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg
     aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc
     agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg
     catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca
     gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt
     tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac
     agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg
     caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg
     tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg
     cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga
     aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta
     ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc
     agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc
     ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata
     ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg
     gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac
     tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg
     acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat
     ttcagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac
     cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa
     acacaaactt ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct
     acttcccgtt tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg
     gtattatttt tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc
     tttctgacaa actcgggctg cgcaaatacc tgctgtggat tattaccggc atgttagtga
     tgtttgcgcc gttctttatt tttatcttcg ggccactgtt acaatacaac attttagtag
     gatcgattgt tggtggtatt tatctaggct tttgttttaa cgccggtgcg ccagcagtag
     aggcatttat tgagaaagtc agccgtcgca gtaatttcga atttggtcgc gcgcggatgt
     ttggctgtgt tggctgggcg ctgtgtgcct cgattgtcgg catcatgttc accatcaata
     atcagtttgt tttctggctg ggctctggct gtgcactcat cctcgccgtt ttactctttt
     tcgccaaaac ggatgcgccc tcttctgcca cggttgccaa tgcggtaggt gccaaccatt
     cggcatttag ccttaagctg gcactggaac tgttcagaca gccaaaactg tggtttttgt
     cactgtatgt tattggcgtt tcctgcacct acgatgtttt tgaccaacag tttgctaatt
     tctttacttc gttctttgct accggtgaac agggtacgcg ggtatttggc tacgtaacga
     caatgggcga attacttaac gcctcgatta tgttctttgc gccactgatc attaatcgca
     tcggtgggaa aaacgccctg ctgctggctg gcactattat gtctgtacgt attattggct
     catcgttcgc cacctcagcg ctggaagtgg ttattctgaa aacgctgcat atgtttgaag
     taccgttcct gctggtgggc tgctttaaat atattaccag ccagtttgaa gtgcgttttt
     cagcgacgat ttatctggtc tgtttctgct tctttaagca actggcgatg atttttatgt
     ctgtactggc gggcaatatg tatgaaagca tcggtttcca gggcgcttat ctggtgctgg
     gtctggtggc gctgggcttc accttaattt ccgtgttcac gcttagcggc cccggcccgc
     tttccctgct gcgtcgtcag gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg
     cgacgcttat ccgaccaaca tatcataacg gagtgatcgc attgaacatg ccaatgaccg
     aaagaataag agcaggcaag ctatttaccg atatgtgcga aggcttaccg gaaaaaagac
     ttcgtgggaa aacgttaatg tatgagttta atcactcgca tccatcagaa gttgaaaaaa
     gagaaagcct gattaaagaa atgtttgcca cggtagggga aaacgcctgg gtagaaccgc
     ctgtctattt ctcttacggt tccaacatcc atataggccg caatttttat gcaaatttca
     atttaaccat tgtcgatgac tacacggtaa caatcggtga taacgtactg attgcaccca
     acgttactct ttccgttacg ggacaccctg tacaccatga attgagaaaa aacggcgaga
     tgtactcttt tccgataacg attggcaata acgtctggat cggaagtcat gtggttatta
     atccaggcgt caccatcggg gataattctg ttattggcgc gggtagtatc gtcacaaaag
     acattccacc aaacgtcgtg gcggctggcg ttccttgtcg ggttattcgc gaaataaacg
     accgggataa gcactattat ttcaaagatt ataaagttga atcgtcagtt taaattataa
     aaattgcctg atacgctgcg cttatcaggc ctacaagttc agcgatctac attagccgca
     tccggcatga acaaagcgca ggaacaagcg tcgcatcatg cctctttgac ccacagctgc
     ggaaaacgta ctggtgcaaa acgcagggtt atgatcatca gcccaacgac gcacagcgca
     tgaaatgccc agtccatcag gtaattgccg ctgatactac gcagcacgcc agaaaaccac
     ggggcaagcc cggcgatgat aaaaccgatt ccctgcataa acgccaccag cttgccagca
     atagccggtt gcacagagtg atcgagcgcc agcagcaaac agagcggaaa cgcgccgccc
     agacctaacc cacacaccat cgcccacaat accggcaatt gcatcggcag ccagataaag
     ccgcagaacc ccaccagttg taacaccagc gccagcatta acagtttgcg ccgatcctga
     tggcgagcca tagcaggcat cagcaaagct cctgcggctt gcccaagcgt catcaatgcc
     agtaaggaac cgctgtactg cgcgctggca ccaatctcaa tatagaaagc gggtaaccag
     gcaatcaggc tggcgtaacc gccgttaatc agaccgaagt aaacacccag cgtccacgcg
     cggggagtga ataccacgcg aaccggagtg gttgttgtct tgtgggaaga ggcgacctcg
     cgggcgcttt gccaccacca ggcaaagagc gcaacaacgg caggcagcgc caccaggcga
     gtgtttgata ccaggtttcg ctatgttgaa ctaaccaggg cgttatggcg gcaccaagcc
     caccgccgcc catcagagcc gcggaccaca gccccatcac cagtggcgtg cgctgctgaa
     accgccgttt aatcaccgaa gcatcaccgc ctgaatgatg ccgatcccca ccccaccaag
     cagtgcgctg ctaagcagca gcgcactttg cgggtaaagc tcacgcatca atgcaccgac
     ggcaatcagc aacagactga tggcgacact gcgacgttcg ctgacatgct gatgaagcca
     gcttccggcc agcgccagcc cgcccatggt aaccaccggc agagcggtaa
//