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ID   PSKS105    preliminary; circular DNA; SYN; 10121 BP.
XX
AC   K01456;
XX
DT   07-NOV-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSKS105 - complete, lacZ fragment.
XX
KW   cloning vector; ampicillin resistance; beta-galactosidase;
KW   drug resistance gene;
KW   galactosidase; lac operon; lacZ gene; promoter region.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-60
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
RN   [2]
RC   pMC1513 from pMC1403 & pKB252, lacUV5 promoter
RC   pSKS104 from pMC1403 & M13mp7
RC   pSKS105 from pMC1403 & M13mp8
RC   pSKS106 from pMC1403 & M13mp9
RC   pMC1585 from pMC1403 & yeast 2 micron & URA3 gene
RC   pMC1587 from pMC1403 & yeast 2 micron & LEU2 gene
RC   pMC1790 from pMC1403 & ARS1 & TRP1 gene
RC   pMC2010 from pMC1403 & yeast 2 micron & TRP1 gene
RC   pMC931 from pMC1403 & pACYC177
RC   pMC1871 from pMC1403 & M13mp7
RC   [pMC1364 from pMC series]
RC   [pMC1396 from pMC series]
RC   [pMC1511 from pMC series]
RC   [pMC1571 from pMC1511]
RC   [pMC1981 from pMC1871]
RA   Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J.;
RT   "Beta-galactosidase gene fusions for analyzing gene expression in
RT   Escherichia coli and yeast";
RL   Meth. Enzymol. 100:293-308(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Plasmid vector pMC1403 has unique EcoRI, SmaI, and BamHI sites
CC   within the first 24 bp of the beta-galactosidase gene, lacZ.
CC   Plasmid pUC8 is a deleted pBR322 plasmid which is ampicillin
CC   resistant and contains the HaeII lac'IPOZ' segments from M13mp8.
CC   pUC8 has polyrestriction insertions in the fifth and sixth codons
CC   of lacZ which are in phase and do not abolish beta-galactosidase
CC   activity. A 265 bp PvuII lac segment from this plasmid was
CC   incorporated into plasmid pMC1403 by DNA cloning and homologous
CC   recombination within lacZ to form plasmid pSKS105.
CC   NCBI gi: 209312
CC   NM (pSKS105)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)(pMC1403)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMC1403 SmaI 9820bp 3647..3647, MCS
FT                   2. pUC8 PvuII-PvuII 301bp 55..356,
FT                   \ lac promoter [265bp]
FT                   -> pSKS105 10121bp"
FT   -               1..3646
FT                   /note="pMC1403 1..3646 3646bp
FT                   SmaI = XmaI = CCC^GGG
FT                   PvuII =       CAG^CTG"
FT   -               3647..3947
FT                   /note="pUC8 55..355 301bp
FT                   PvuII =       CAG^CTG
FT                   SmaI = XmaI = CCC^GGG"
FT   -               3948..10121
FT                   /note="pMC1403 3647..9820 6174bp"
FT   CDS             1..>60
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ);
FT                   NCBI gi: 209313"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
XX
SQ   Sequence 10121 BP; 2370 A; 2715 C; 2642 G; 2394 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcccctgg cacgacaggt
     ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     gataacaatt tcacacagga aacagctatg accatgatta cgaattcccg gggatccgtc
     gacctgcagc caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagggg gatcccgtcg
     ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
     cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
     ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca
     atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
     atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt
     ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg
     aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc
     gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc
     gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc
     gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt
     tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg
     gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
     cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc
     gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg
     tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
     tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
     agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt
     cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg
     tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc
     cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc
     acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg
     cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg
     gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc
     agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga
     cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg
     ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact
     gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg
     gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg
     accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc
     gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg
     agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc
     tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga
     gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag
     aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc
     tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg
     agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga
     ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg
     ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct
     ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac
     ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat
     ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg
     ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc
     aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc
     ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg
     tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc
     agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa
     ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc
     atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa tttcagctga
     gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc
     catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact
     tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt
     ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt
     ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca
     aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg atgtttgcgc
     cgttctttat ttttatcttc gggccactgt tacaatacaa cattttagta ggatcgattg
     ttggtggtat ttatctaggc ttttgtttta acgccggtgc gccagcagta gaggcattta
     ttgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg tttggctgtg
     ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat aatcagtttg
     ttttctggct gggctctggc tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa
     cggatgcgcc ctcttctgcc acggttgcca atgcggtagg tgccaaccat tcggcattta
     gccttaagct ggcactggaa ctgttcagac agccaaaact gtggtttttg tcactgtatg
     ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat ttctttactt
     cgttctttgc taccggtgaa cagggtacgc gggtatttgg ctacgtaacg acaatgggcg
     aattacttaa cgcctcgatt atgttctttg cgccactgat cattaatcgc atcggtggga
     aaaacgccct gctgctggct ggcactatta tgtctgtacg tattattggc tcatcgttcg
     ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa gtaccgttcc
     tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt tcagcgacga
     tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg tctgtactgg
     cgggcaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg ggtctggtgg
     cgctgggctt caccttaatt tccgtgttca cgcttagcgg ccccggcccg ctttccctgc
     tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc gcgacgctta
     tccgaccaac atatcataac ggagtgatcg cattgaacat gccaatgacc gaaagaataa
     gagcaggcaa gctatttacc gatatgtgcg aaggcttacc ggaaaaaaga cttcgtggga
     aaacgttaat gtatgagttt aatcactcgc atccatcaga agttgaaaaa agagaaagcc
     tgattaaaga aatgtttgcc acggtagggg aaaacgcctg ggtagaaccg cctgtctatt
     tctcttacgg ttccaacatc catataggcc gcaattttta tgcaaatttc aatttaacca
     ttgtcgatga ctacacggta acaatcggtg ataacgtact gattgcaccc aacgttactc
     tttccgttac gggacaccct gtacaccatg aattgagaaa aaacggcgag atgtactctt
     ttccgataac gattggcaat aacgtctgga tcggaagtca tgtggttatt aatccaggcg
     tcaccatcgg ggataattct gttattggcg cgggtagtat cgtcacaaaa gacattccac
     caaacgtcgt ggcggctggc gttccttgtc gggttattcg cgaaataaac gaccgggata
     agcactatta tttcaaagat tataaagttg aatcgtcagt ttaaattata aaaattgcct
     gatacgctgc gcttatcagg cctacaagtt cagcgatcta cattagccgc atccggcatg
     aacaaagcgc aggaacaagc gtcgcatcat gcctctttga cccacagctg cggaaaacgt
     actggtgcaa aacgcagggt tatgatcatc agcccaacga cgcacagcgc atgaaatgcc
     cagtccatca ggtaattgcc gctgatacta cgcagcacgc cagaaaacca cggggcaagc
     ccggcgatga taaaaccgat tccctgcata aacgccacca gcttgccagc aatagccggt
     tgcacagagt gatcgagcgc cagcagcaaa cagagcggaa acgcgccgcc cagacctaac
     ccacacacca tcgcccacaa taccggcaat tgcatcggca gccagataaa gccgcagaac
     cccaccagtt gtaacaccag cgccagcatt aacagtttgc gccgatcctg atggcgagcc
     atagcaggca tcagcaaagc tcctgcggct tgcccaagcg tcatcaatgc cagtaaggaa
     ccgctgtact gcgcgctggc accaatctca atatagaaag cgggtaacca ggcaatcagg
     ctggcgtaac cgccgttaat cagaccgaag taaacaccca gcgtccacgc gcggggagtg
     aataccacgc gaaccggagt ggttgttgtc ttgtgggaag aggcgacctc gcgggcgctt
     tgccaccacc aggcaaagag cgcaacaacg gcaggcagcg ccaccaggcg agtgtttgat
     accaggtttc gctatgttga actaaccagg gcgttatggc ggcaccaagc ccaccgccgc
     ccatcagagc cgcggaccac agccccatca ccagtggcgt gcgctgctga aaccgccgtt
     taatcaccga agcatcaccg cctgaatgat gccgatcccc accccaccaa gcagtgcgct
     gctaagcagc agcgcacttt gcgggtaaag ctcacgcatc aatgcaccga cggcaatcag
     caacagactg atggcgacac tgcgacgttc gctgacatgc tgatgaagcc agcttccggc
     cagcgccagc ccgcccatgg taaccaccgg cagagcggta a
//