Return to this vector's summary.
ID PSKS106 preliminary; circular DNA; SYN; 10121 BP.
XX
AC K01457;
XX
DT 07-NOV-1984 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pSKS106 - complete, lacZ fragment.
XX
KW cloning vector; ampicillin resistance; beta-galactosidase;
KW drug resistance gene;
KW galactosidase; lac operon; lacZ gene; promoter region.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RP 1-60
RC pCV1 from pBR322 & linker
RC pSKS101 from pUC7 & Tn5
RC pSKS114 from pUC7 & Tn9
RC pMC1827, pMC1828 from pMC1403 & pSKS101
RC pMC1843 from pMC1827
RC pMLB1034 from pMC1403
RC pMC1844 from pMLB1034 & pUC7
RC pSKS104 from pMC1403 & pUC7
RC pSKS105 from pMC1403 & pUC8
RC pSKS106 from pMC1403 & pUC9
RC pSKS107 from pSKS105
RC pFR10 from pSKS106 & pCV1
RC pFR97 from pSKS104 & pFR10
RC pFR98 from pSKS105 & pFR10
RC pFR109 from pSKS106 & pFR10
RC plasmid from pMC279 & pMC1403
RC plasmid2 from plasmid & pSKS101
RC pMC1871 from plasmid2 & pMC1843
RA Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT "New versatile plasmid vectors for expression of hybrid proteins
RT coded by a cloned gene fused to lacZ gene sequences encoding an
RT enzymatically active carboxy-terminal portion of beta-galactosidase";
RL Gene 25:71-82(1983).
XX
RN [2]
RC pMC1513 from pMC1403 & pKB252, lacUV5 promoter
RC pSKS104 from pMC1403 & M13mp7
RC pSKS105 from pMC1403 & M13mp8
RC pSKS106 from pMC1403 & M13mp9
RC pMC1585 from pMC1403 & yeast 2 micron & URA3 gene
RC pMC1587 from pMC1403 & yeast 2 micron & LEU2 gene
RC pMC1790 from pMC1403 & ARS1 & TRP1 gene
RC pMC2010 from pMC1403 & yeast 2 micron & TRP1 gene
RC pMC931 from pMC1403 & pACYC177
RC pMC1871 from pMC1403 & M13mp7
RC [pMC1364 from pMC series]
RC [pMC1396 from pMC series]
RC [pMC1511 from pMC series]
RC [pMC1571 from pMC1511]
RC [pMC1981 from pMC1871]
RA Casadaban M.J., Martinez-Arias A., Shapira S.K., Chou J.;
RT "Beta-galactosidase gene fusions for analyzing gene expression in
RT Escherichia coli and yeast";
RL Meth. Enzymol. 100:293-308(1983).
XX
CC Created by Moore, July 1995, under contract with NCBI.
CC Plasmid vector pMC1403 has unique EcoRI, SmaI, and BamHI sites
CC within the first 24 bp of the beta-galactosidase gene, lacZ.
CC Plasmid pUC9 is a deleted pBR322 plasmid which is ampicillin
CC resistant and contains the HaeII lac'IPOZ' segments from M13mp9.
CC pUC9 has polyrestriction insertions in the fifth and sixth codons
CC of lacZ which are in phase and do not abolish beta-galactosidase
CC activity. A 265 bp PvuII lac segment from this plasmid was
CC incorporated into plasmid pMC1403 by DNA cloning and homologous
CC recombination within lacZ to form plasmid pSKS104.
CC NCBI gi: 209314
CC NM (pSKS106)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUC9)(pMC1403)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pMC1403 SmaI 9820bp 3647..3647, MCS
FT 2. pUC9 PvuII-PvuII 301bp 55..356,lac promoter [265bp]
FT -> pSKS106 10121bp"
FT - 1..3646
FT /note="pMC1403 1..3646 3646bp
FT SmaI = XmaI = CCC^GGG
FT PvuII = CAG^CTG"
FT - 3647..3947
FT /note="pUC9 55..355 301bp
FT PvuII = CAG^CTG
FT SmaI = XmaI = CCC^GGG"
FT - 3948..10121
FT /note="pMC1403 3647..9820 6174bp"
FT CDS 1..>60
FT /note="GEN E. coli beta-galactosidase gene (lacZ);
FT NCBI gi: 209315"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT EcoRI"
FT misc_binding 0..0
FT /note="SIT SmaI"
FT misc_binding 0..0
FT /note="SIT BamHI"
XX
SQ Sequence 10121 BP; 2370 A; 2714 C; 2643 G; 2394 T; 0 other;
ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcccctgg cacgacaggt
ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt ggctgcaggt
cgacggatcc ccgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagggg gatcccgtcg
ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc
cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact
ggcagatgca cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca
atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg
atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt
ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg
aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc
gttggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc
gtgacgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc
gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt
tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg
gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca
cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc
gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg
tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc
tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc
agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt
cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg
tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc
cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc
acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg
cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg
gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc
agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga
cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg
ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact
gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg
gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg
accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc
gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg
agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc
tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga
gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag
aagccgggca catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc
tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg
agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga
ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg
ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct
ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac
ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat
ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg
ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc
aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc
ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg
tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc
agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa
ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc
atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa tttcagctga
gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc
catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa aacacaaact
tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc tacttcccgt
ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg ggtattattt
ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg ctttctgaca
aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg atgtttgcgc
cgttctttat ttttatcttc gggccactgt tacaatacaa cattttagta ggatcgattg
ttggtggtat ttatctaggc ttttgtttta acgccggtgc gccagcagta gaggcattta
ttgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg tttggctgtg
ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat aatcagtttg
ttttctggct gggctctggc tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa
cggatgcgcc ctcttctgcc acggttgcca atgcggtagg tgccaaccat tcggcattta
gccttaagct ggcactggaa ctgttcagac agccaaaact gtggtttttg tcactgtatg
ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat ttctttactt
cgttctttgc taccggtgaa cagggtacgc gggtatttgg ctacgtaacg acaatgggcg
aattacttaa cgcctcgatt atgttctttg cgccactgat cattaatcgc atcggtggga
aaaacgccct gctgctggct ggcactatta tgtctgtacg tattattggc tcatcgttcg
ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa gtaccgttcc
tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt tcagcgacga
tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg tctgtactgg
cgggcaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg ggtctggtgg
cgctgggctt caccttaatt tccgtgttca cgcttagcgg ccccggcccg ctttccctgc
tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc gcgacgctta
tccgaccaac atatcataac ggagtgatcg cattgaacat gccaatgacc gaaagaataa
gagcaggcaa gctatttacc gatatgtgcg aaggcttacc ggaaaaaaga cttcgtggga
aaacgttaat gtatgagttt aatcactcgc atccatcaga agttgaaaaa agagaaagcc
tgattaaaga aatgtttgcc acggtagggg aaaacgcctg ggtagaaccg cctgtctatt
tctcttacgg ttccaacatc catataggcc gcaattttta tgcaaatttc aatttaacca
ttgtcgatga ctacacggta acaatcggtg ataacgtact gattgcaccc aacgttactc
tttccgttac gggacaccct gtacaccatg aattgagaaa aaacggcgag atgtactctt
ttccgataac gattggcaat aacgtctgga tcggaagtca tgtggttatt aatccaggcg
tcaccatcgg ggataattct gttattggcg cgggtagtat cgtcacaaaa gacattccac
caaacgtcgt ggcggctggc gttccttgtc gggttattcg cgaaataaac gaccgggata
agcactatta tttcaaagat tataaagttg aatcgtcagt ttaaattata aaaattgcct
gatacgctgc gcttatcagg cctacaagtt cagcgatcta cattagccgc atccggcatg
aacaaagcgc aggaacaagc gtcgcatcat gcctctttga cccacagctg cggaaaacgt
actggtgcaa aacgcagggt tatgatcatc agcccaacga cgcacagcgc atgaaatgcc
cagtccatca ggtaattgcc gctgatacta cgcagcacgc cagaaaacca cggggcaagc
ccggcgatga taaaaccgat tccctgcata aacgccacca gcttgccagc aatagccggt
tgcacagagt gatcgagcgc cagcagcaaa cagagcggaa acgcgccgcc cagacctaac
ccacacacca tcgcccacaa taccggcaat tgcatcggca gccagataaa gccgcagaac
cccaccagtt gtaacaccag cgccagcatt aacagtttgc gccgatcctg atggcgagcc
atagcaggca tcagcaaagc tcctgcggct tgcccaagcg tcatcaatgc cagtaaggaa
ccgctgtact gcgcgctggc accaatctca atatagaaag cgggtaacca ggcaatcagg
ctggcgtaac cgccgttaat cagaccgaag taaacaccca gcgtccacgc gcggggagtg
aataccacgc gaaccggagt ggttgttgtc ttgtgggaag aggcgacctc gcgggcgctt
tgccaccacc aggcaaagag cgcaacaacg gcaggcagcg ccaccaggcg agtgtttgat
accaggtttc gctatgttga actaaccagg gcgttatggc ggcaccaagc ccaccgccgc
ccatcagagc cgcggaccac agccccatca ccagtggcgt gcgctgctga aaccgccgtt
taatcaccga agcatcaccg cctgaatgat gccgatcccc accccaccaa gcagtgcgct
gctaagcagc agcgcacttt gcgggtaaag ctcacgcatc aatgcaccga cggcaatcag
caacagactg atggcgacac tgcgacgttc gctgacatgc tgatgaagcc agcttccggc
cagcgccagc ccgcccatgg taaccaccgg cagagcggta a
//