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ID   PSKS107    preliminary; circular DNA; SYN; 9938 BP.
XX
AC   K01451;
XX
DT   07-NOV-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSKS107 - complete, no lac promoter.
XX
KW   cloning vector; beta-galactosidase; galactosidase; lac operon;
KW   lacZ gene.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-46
RC   pCV1 from pBR322 & linker
RC   pSKS101 from pUC7 & Tn5
RC   pSKS114 from pUC7 & Tn9
RC   pMC1827, pMC1828 from pMC1403 & pSKS101
RC   pMC1843 from pMC1827
RC   pMLB1034 from pMC1403
RC   pMC1844 from pMLB1034 & pUC7
RC   pSKS104 from pMC1403 & pUC7
RC   pSKS105 from pMC1403 & pUC8
RC   pSKS106 from pMC1403 & pUC9
RC   pSKS107 from pSKS105
RC   pFR10 from pSKS106 & pCV1
RC   pFR97 from pSKS104 & pFR10
RC   pFR98 from pSKS105 & pFR10
RC   pFR109 from pSKS106 & pFR10
RC   plasmid from pMC279 & pMC1403
RC   plasmid2 from plasmid & pSKS101
RC   pMC1871 from plasmid2 & pMC1843
RA   Shapira S.K., Chou J., Richaud F.V., Casadaban M.J.;
RT   "New versatile plasmid vectors for expression of hybrid proteins
RT   coded by a cloned gene fused to lacZ gene sequences encoding an
RT   enzymatically active carboxy-terminal portion of beta-galactosidase";
RL   Gene 25:71-82(1983).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Plasmid pSKS107 was formed by removing the lac promoter and
CC   translation initiation region from the pSKS105 plasmid by EcoRI
CC   digestion and religation.
CC   NCBI gi: 209316
CC   NM (pSKS107)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSKS105)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSKS105 remove small EcoRI-EcoRI 183bp
FT                   \ 3640..3647 & 55..231, lac promoter/9838bp
FT                   \ pUC8 EcoRI 231..231/pMC1403 EcoRI 3640..3640
FT                   -> pSKS107 9838bp"
FT   -               1..3639
FT                   /note="pMC1403 1..3639 3639bp
FT                   EcoRI = G^AATTC"
FT   -               3640..3764
FT                   /note="pUC8 231..355 125bp
FT                   PvuII =       CAG^CTG
FT                   SmaI = XmaI = CCC^GGG"
FT   -               3765..9938
FT                   /note="pMC1403 3647..9820 6174bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 9938 BP; 2322 A; 2671 C; 2597 G; 2348 T; 0 other;
     ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc
     tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg
     taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg
     ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat
     tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat
     atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc
     ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
     ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
     ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
     aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
     atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg
     ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt
     catcggtatc attaccccca tgaacagaaa ttccccctta cacggaggca tcaagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attcccgggg atccgtcgac
     ctgcagccaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagggggat cccgtcgttt
     tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
     aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
     agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc
     cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
     aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc
     atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat
     ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt
     ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg
     acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct
     ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc
     gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca
     ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac
     tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg
     cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg
     gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga
     ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga
     cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc
     attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg
     atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc
     gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc
     cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc
     tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag
     ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc
     ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc
     gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta
     aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg
     tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg
     attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc
     gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt
     tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc
     tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg
     atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg
     ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag
     ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc
     ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc
     tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg
     gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata
     acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga
     aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg
     ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta
     tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg
     aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg
     tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta
     ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct
     tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattt cagctgagcg
     ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat
     gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaaaaac acaaactttt
     ggatgttcgg tttattcttt ttcttttact tttttatcat gggagcctac ttcccgtttt
     tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacgggt attatttttg
     ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
     tcgggctgcg caaatacctg ctgtggatta ttaccggcat gttagtgatg tttgcgccgt
     tctttatttt tatcttcggg ccactgttac aatacaacat tttagtagga tcgattgttg
     gtggtattta tctaggcttt tgttttaacg ccggtgcgcc agcagtagag gcatttattg
     agaaagtcag ccgtcgcagt aatttcgaat ttggtcgcgc gcggatgttt ggctgtgttg
     gctgggcgct gtgtgcctcg attgtcggca tcatgttcac catcaataat cagtttgttt
     tctggctggg ctctggctgt gcactcatcc tcgccgtttt actctttttc gccaaaacgg
     atgcgccctc ttctgccacg gttgccaatg cggtaggtgc caaccattcg gcatttagcc
     ttaagctggc actggaactg ttcagacagc caaaactgtg gtttttgtca ctgtatgtta
     ttggcgtttc ctgcacctac gatgtttttg accaacagtt tgctaatttc tttacttcgt
     tctttgctac cggtgaacag ggtacgcggg tatttggcta cgtaacgaca atgggcgaat
     tacttaacgc ctcgattatg ttctttgcgc cactgatcat taatcgcatc ggtgggaaaa
     acgccctgct gctggctggc actattatgt ctgtacgtat tattggctca tcgttcgcca
     cctcagcgct ggaagtggtt attctgaaaa cgctgcatat gtttgaagta ccgttcctgc
     tggtgggctg ctttaaatat attaccagcc agtttgaagt gcgtttttca gcgacgattt
     atctggtctg tttctgcttc tttaagcaac tggcgatgat ttttatgtct gtactggcgg
     gcaatatgta tgaaagcatc ggtttccagg gcgcttatct ggtgctgggt ctggtggcgc
     tgggcttcac cttaatttcc gtgttcacgc ttagcggccc cggcccgctt tccctgctgc
     gtcgtcaggt gaatgaagtc gcttaagcaa tcaatgtcgg atgcggcgcg acgcttatcc
     gaccaacata tcataacgga gtgatcgcat tgaacatgcc aatgaccgaa agaataagag
     caggcaagct atttaccgat atgtgcgaag gcttaccgga aaaaagactt cgtgggaaaa
     cgttaatgta tgagtttaat cactcgcatc catcagaagt tgaaaaaaga gaaagcctga
     ttaaagaaat gtttgccacg gtaggggaaa acgcctgggt agaaccgcct gtctatttct
     cttacggttc caacatccat ataggccgca atttttatgc aaatttcaat ttaaccattg
     tcgatgacta cacggtaaca atcggtgata acgtactgat tgcacccaac gttactcttt
     ccgttacggg acaccctgta caccatgaat tgagaaaaaa cggcgagatg tactcttttc
     cgataacgat tggcaataac gtctggatcg gaagtcatgt ggttattaat ccaggcgtca
     ccatcgggga taattctgtt attggcgcgg gtagtatcgt cacaaaagac attccaccaa
     acgtcgtggc ggctggcgtt ccttgtcggg ttattcgcga aataaacgac cgggataagc
     actattattt caaagattat aaagttgaat cgtcagttta aattataaaa attgcctgat
     acgctgcgct tatcaggcct acaagttcag cgatctacat tagccgcatc cggcatgaac
     aaagcgcagg aacaagcgtc gcatcatgcc tctttgaccc acagctgcgg aaaacgtact
     ggtgcaaaac gcagggttat gatcatcagc ccaacgacgc acagcgcatg aaatgcccag
     tccatcaggt aattgccgct gatactacgc agcacgccag aaaaccacgg ggcaagcccg
     gcgatgataa aaccgattcc ctgcataaac gccaccagct tgccagcaat agccggttgc
     acagagtgat cgagcgccag cagcaaacag agcggaaacg cgccgcccag acctaaccca
     cacaccatcg cccacaatac cggcaattgc atcggcagcc agataaagcc gcagaacccc
     accagttgta acaccagcgc cagcattaac agtttgcgcc gatcctgatg gcgagccata
     gcaggcatca gcaaagctcc tgcggcttgc ccaagcgtca tcaatgccag taaggaaccg
     ctgtactgcg cgctggcacc aatctcaata tagaaagcgg gtaaccaggc aatcaggctg
     gcgtaaccgc cgttaatcag accgaagtaa acacccagcg tccacgcgcg gggagtgaat
     accacgcgaa ccggagtggt tgttgtcttg tgggaagagg cgacctcgcg ggcgctttgc
     caccaccagg caaagagcgc aacaacggca ggcagcgcca ccaggcgagt gtttgatacc
     aggtttcgct atgttgaact aaccagggcg ttatggcggc accaagccca ccgccgccca
     tcagagccgc ggaccacagc cccatcacca gtggcgtgcg ctgctgaaac cgccgtttaa
     tcaccgaagc atcaccgcct gaatgatgcc gatccccacc ccaccaagca gtgcgctgct
     aagcagcagc gcactttgcg ggtaaagctc acgcatcaat gcaccgacgg caatcagcaa
     cagactgatg gcgacactgc gacgttcgct gacatgctga tgaagccagc ttccggccag
     cgccagcccg cccatggtaa ccaccggcag agcggtaa
//