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ID   PSKS108    preliminary; circular DNA; SYN; 4019 BP.
XX
AC   IG9801;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pSKS108 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSKS103 from pBR322 & pTK2
RC   pSKS108 from pSKS105 & pSKS103
RC   pSKS116 from pSKS108
RC   pSKS111 from pSKS116 & pSKS106, lacZgene
RC   pMC1033 from Tn3 tnpR gene
RC   pSKS121 from pSKS116 & pMC1033
RC   pSKS114 from pSKS116 & Tn9 IS1 & pTK2
RC   pSKS122 from pSKS116 & pSKS114
RA   Shapira S.K., Casadaban M.J.;
RT   "Hybrid protein thymidine kinase gene fusions: plasmid vectors for
RT   the study of transcription and translation initiation signals";
RL   Gene 52:83-94(1987).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSKS108)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSKS105)(pSKS103)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. HSV1 BamHI-BamHI 3579bp 45056..48635,
FT                   \ Q frag tk gene/.28 to .32
FT                   \ [tk gene CDS = #X14112 46672..47802]
FT                   -> pTK2 7940bp [7800bp]
FT                   1. pBR322 remove BamHI-PvuII 1691bp 376..2067, 2670bp
FT                   2. pTK2 PvuII-BglII 1788bp 46068..47856, HSV-1 tk gene
FT                   -> pSKS103 4458bp [amp gene]
FT                   1. pSKS105 HincII-HincII 6787bp 6522..10121..3188
FT                   \ pMC1403 HincII = 3188 4070 4694 6522
FT                   \ pUC8 HincII = 249
FT                   2. pSKS103 RsaI-SacI, 5' tk gene
FT                   \ pBR322 RsaI = 166 2284 3849
FT                   \ pTK2 has SacI in pBR322 2067..4361..376
FT                   3. pSKS103 SacI-HincII, 3' tk gene/pBR322 3910
FT                   \ pBR322 HincII = 654 3910
FT                   \ pTK2 has SacI in pBR322 2067..4361..376
FT                   -> pSKS108 4100bp [amp gene]"
FT   -               1..1183
FT                   /note="pSKS105 3188..4370 1183bp
FT                   \ 3188..3646/55..356/3647..4070 1183bp
FT                   HindII = HincII = GTY^RAC"
FT   -               1184..4019
FT                   /note="pSKS103 4009..4462..2382 2836bp
FT                   \ 3910..4361..375/46068..47859/2067..2284 2837bp
FT                   RsaI =             GT^AC
FT                   HindII = HincII = GTY^RAC"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI"
FT   CDS             0..0
FT                   /note="GEN vertebrate thymidine kinase gene (TK);
FT                   truncated"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 4019 BP; 901 A; 1118 C; 1117 G; 883 T; 0 other;
     aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     taggcgtatc acgaggccct ttcgtcttca agaattcccc tggcacgaca ggtttcccga
     ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     atttcacaca ggaaacagct atgaccatga ttacgaattc ccggggatcc gtcgacctgc
     agccaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     cccaacttaa tcgccttgca gcacatcccc ctttcgccag ggggatcccg tcgttttaca
     acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
     ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
     gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
     gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
     ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaacacgg gataataccg
     cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     cctttcgtct tcaagaattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
     agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
     tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
     tacgcgatca tggcgaccac acccgtcctg tgctggccgg ggtcccgcgg aaactcggcc
     gtggtgacca atacaaaaca aaagcgctcc tcgtaccagc gaagaagggg cagagatgcc
     gtagtcaggt ttagttcgtc cggcggcgcc agaaatccgc gcggtggttt ttgggggtcg
     ggggtgtttg gcagccacag acgcccggtg ttcgtgtcgc gccagtacat gcggtccatg
     cccaggccat ccaaaaacca tgggtctgtc tgctcagtcc agtcgtggac ctgaccccac
     gcaacgccca aaataataac ccccacgaac cataaaccat tccccatggg ggaccccgtc
     cctaacccac ggggcccgtg gctatggcag ggcttgccgc cccgacgttg gctgcgagcc
     ctgggccttc acccgaactt gggggttggg gtggggaaaa ggaagaaacg cgggcgtatt
     ggtcccaatg gggtctcggt ggggtatcga cagagtgcca gccctgggac cgaaccccgc
     gtttatgaac aaacgaccca acacccgtgc gttttattct gtctttttat tgccgtcata
     gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc ccatctcccg
     ggcaaacgtg cgcgccaggt cgcagatcgt cggtatggag cctggggtgg tgacgtgggt
     ctggaccatc ccggaggtaa gttgcagcag ggcgtcccgg cagccggcgg gcgattggtc
     gtaatccagg ataaagacat gcatgggacg gaggcgtttg gccaagacgt ccaaagccca
     ggcaaacacg ttatacaggt cgccgttggg ggccagcaac tcgggggccc gaaacagggt
     aaataacgtg tccccgatat ggggtcgtgg gcccgcgttg ctctggggct cggcaccctg
     gggcggcacg gccgcccccg aaagctgtcc ccaatcctcc cgccacgacc cgccgccctg
     cagataccgc accgtattgg caagcagccc ataaacgcgg cgaatcgcgg ccagcatagc
     caggtcaagc cgctcgccgg ggcgctggcg tttggccagg cggtcgatgt gtctgtcctc
     cggaagggcc cccaacacga tgtttgtgcc gggcaaggtc ggcgggatga gggccacgaa
     cgccagcacg gcctgggggg tcatgctgcc cataaggtat cgcgcggccg ggtagcacag
     gagggcggcg atgggatggc ggtcgaagat gagggtgagg gccgggggcg gggcatgtga
     gctcccagcc tcccccccga tatgaggagc cagaacggcg tcggtcacgg cataaggcat
     gcccattgtt atctgggcgc ttgtcattac caccgccgcg tccccggccg atatctcacc
     ctggtcgagg cggtgttgtg tggtgtagat gttcgcgatt gtctcggaag cccccaacac
     ccgccagtaa gtcatcggct cgggtacgta gacgatatcg tcgcgcgaac ccagggccac
     cagcagttgc gtggtggtgg ttttccccat cccgtgggga ccgtctatat aaacccgcag
     tagcgtgggc attttctgct ccaggcggac ttccgtggct ttttgttgcc ggcgagggcg
     caacgccgta cgtcggttgt tatggccgcg agaacgcgca gcctggtcga acgcagacgc
     gtgttgatgg caggggtacg aagccatacg cgcttctaca aggcgctggc cgaagaggtg
     cgggagtttc acgccaccaa gatcctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgt
//