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ID   PSKS116    preliminary; circular DNA; SYN; 3836 BP.
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AC   ATCC37589;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pSKS116 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSKS103 from pBR322 & pTK2
RC   pSKS108 from pSKS105 & pSKS103
RC   pSKS116 from pSKS108
RC   pSKS111 from pSKS116 & pSKS106, lacZgene
RC   pMC1033 from Tn3 tnpR gene
RC   pSKS121 from pSKS116 & pMC1033
RC   pSKS114 from pSKS116 & Tn9 IS1 & pTK2
RC   pSKS122 from pSKS116 & pSKS114
RA   Shapira S.K., Casadaban M.J.;
RT   "Hybrid protein thymidine kinase gene fusions: plasmid vectors for
RT   the study of transcription and translation initiation signals";
RL   Gene 52:83-94(1987).
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RN   [2]
RC   pOGA from pAGO
RA   Kit S., Otsuka H., Qavi H., Kit M.;
RT   "Functional expression of the Herpes simplex virus thymidine kinase
RT   gene in Escherichia coli K-12";
RL   Gene 16:287-295(1981).
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RN   [3]
RC   [pAGO from HSV tk gene]
RC   pMH1 from pAGO
RC   pMH110 from pAGO
RA   Kit S., Otsuka H., Qavi H., Trkula D., Dubbs D.R., Hazen M.;
RT   "Biochemical transformation of thymidine kinase (TK)-deficient mouse
RT   cells by Herpes simplex virus type 1 DNA fragments purified from
RT   hybrid plasmids";
RL   Nucleic Acids Res. 8:5233-5253(1980).
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RN   [4]
RC   pMB9-Ecotdk from pMB9 & E.coli tdk gene
RC   plasmid from pTK1 & pMB9-Ecotdk & linker
RC   pTK3 from plasmid & pBR322
RA   Subramani S.;
RT   ;
RL   Unpublished (1981).
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RN   [5]
RC   pTK1 from HSV-1 tk gene
RA   Goff S.P., Berg P.;
RT   "Construction, propagation, and expression of simian virus 40
RT   recombinanbt genomes containing the Escherichia coli gene for
RT   thymidine kinase and a Saccharomyces cerevisiae gene for
RT   tyrosine transfer RNA";
RL   J. Mol. Biol. 133:359-383(1979).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--3.8; BamHI--3.8; HindIII--uncut; HincII--3.8. (ATCC staff)
CC   This is a vector for the construction of thymidine kinase fusion
CC   proteins.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSKS116)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSKS108)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. HSV1 BamHI-BamHI 3579bp 45056..48635,
FT                   \ Q frag tk gene/.28 to .32
FT                   \ [tk gene CDS = #X14112 46672..47802]
FT                   -> pTK2 7940bp [7800bp]
FT                   1. pBR322 remove BamHI-PvuII 1691bp 376..2067, 2670bp
FT                   2. pTK2 PvuII-BglII 1788bp 46068..47856, HSV-1 tk gene
FT                   -> pSKS103 4458bp [amp gene]
FT                   1. pSKS105 HincII-HincII 6787bp 6522..10121..3188
FT                   \ pMC1403 HincII = 3188 4070 4694 6522
FT                   \ pUC8 HincII = 249
FT                   2. pSKS103 RsaI-SacI, 5' tk gene
FT                   \ pBR322 RsaI = 166 2284 3849
FT                   \ pTK2 has SacI in pBR322 2067..4361..376
FT                   3. pSKS103 SacI-HincII, 3' tk gene/pBR322 3910
FT                   \ pBR322 HincII = 654 3910
FT                   \ pTK2 has SacI in pBR322 2067..4361..376
FT                   -> pSKS108 4100bp [amp gene]
FT                   1. pSKS108 remove EcoRI-EcoRI, lacPOZ' region
FT                   \ pMC1403 EcoRI 3640/pUC8 EcoRI 231
FT                   -> pSKS116 3800bp"
FT   -               1..452
FT                   /note="pSKS108 1..452 452bp
FT                   \ 3640..3646/55..231
FT                   EcoRI = G^AATTC"
FT   -               453..3836
FT                   /note="pSKS108 636..4019 3384bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-BamHI"
FT   CDS             0..0
FT                   /note="GEN vertebrate thymidine kinase gene (TK);
FT                   truncated"
FT   misc_binding    400..400
FT                   /note="SIT SacI"
FT   misc_binding    700..700
FT                   /note="SIT PstI"
FT   misc_binding    1000..1000
FT                   /note="SIT SmaI"
FT   misc_binding    1400..1400
FT                   /note="SIT PvuII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3100..3100
FT                   /note="SIT PstI"
FT   misc_binding    3400..3400
FT                   /note="SIT HincII"
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SQ   Sequence 3836 BP; 853 A; 1074 C; 1072 G; 837 T; 0 other;
     aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     taggcgtatc acgaggccct ttcgtcttca agaattcccg gggatccgtc gacctgcagc
     caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     aacttaatcg ccttgcagca catccccctt tcgccagggg gatcccgtcg ttttacaacg
     tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
     gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
     cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt
     tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
     gctacaggaa ggccagacgc gaattatttt tgatggcgtt aacacgggat aataccgcgc
     cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtcttca agaattctca tgtttgacag cttatcatcg ataagcttta atgcggtagt
     ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc
     atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta
     ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg
     ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc
     gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac
     gcgatcatgg cgaccacacc cgtcctgtgc tggccggggt cccgcggaaa ctcggccgtg
     gtgaccaata caaaacaaaa gcgctcctcg taccagcgaa gaaggggcag agatgccgta
     gtcaggttta gttcgtccgg cggcgccaga aatccgcgcg gtggtttttg ggggtcgggg
     gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc
     aggccatcca aaaaccatgg gtctgtctgc tcagtccagt cgtggacctg accccacgca
     acgcccaaaa taataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct
     aacccacggg gcccgtggct atggcagggc ttgccgcccc gacgttggct gcgagccctg
     ggccttcacc cgaacttggg ggttggggtg gggaaaagga agaaacgcgg gcgtattggt
     cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
     tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
     cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccgggc
     aaacgtgcgc gccaggtcgc agatcgtcgg tatggagcct ggggtggtga cgtgggtctg
     gaccatcccg gaggtaagtt gcagcagggc gtcccggcag ccggcgggcg attggtcgta
     atccaggata aagacatgca tgggacggag gcgtttggcc aagacgtcca aagcccaggc
     aaacacgtta tacaggtcgc cgttgggggc cagcaactcg ggggcccgaa acagggtaaa
     taacgtgtcc ccgatatggg gtcgtgggcc cgcgttgctc tggggctcgg caccctgggg
     cggcacggcc gcccccgaaa gctgtcccca atcctcccgc cacgacccgc cgccctgcag
     ataccgcacc gtattggcaa gcagcccata aacgcggcga atcgcggcca gcatagccag
     gtcaagccgc tcgccggggc gctggcgttt ggccaggcgg tcgatgtgtc tgtcctccgg
     aagggccccc aacacgatgt ttgtgccggg caaggtcggc gggatgaggg ccacgaacgc
     cagcacggcc tggggggtca tgctgcccat aaggtatcgc gcggccgggt agcacaggag
     ggcggcgatg ggatggcggt cgaagatgag ggtgagggcc gggggcgggg catgtgagct
     cccagcctcc cccccgatat gaggagccag aacggcgtcg gtcacggcat aaggcatgcc
     cattgttatc tgggcgcttg tcattaccac cgccgcgtcc ccggccgata tctcaccctg
     gtcgaggcgg tgttgtgtgg tgtagatgtt cgcgattgtc tcggaagccc ccaacacccg
     ccagtaagtc atcggctcgg gtacgtagac gatatcgtcg cgcgaaccca gggccaccag
     cagttgcgtg gtggtggttt tccccatccc gtggggaccg tctatataaa cccgcagtag
     cgtgggcatt ttctgctcca ggcggacttc cgtggctttt tgttgccggc gagggcgcaa
     cgccgtacgt cggttgttat ggccgcgaga acgcgcagcc tggtcgaacg cagacgcgtg
     ttgatggcag gggtacgaag ccatacgcgc ttctacaagg cgctggccga agaggtgcgg
     gagtttcacg ccaccaagat cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgt
//