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ID PSL1180 preliminary; circular DNA; SYN; 3422 BP.
XX
AC IG0323;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pSL1180 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pKK421-1 from pKK233-2
RC pUC120 from pUC118 & oligo ATG
RC pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC pSL180 from pUC18 & linker
RC pSL189 from pUC19 & linker
RC pSL190 from pSL189
RC pSL1180 from pUC118 & linker
RC pSL1190 from pUC119 & linker
RC pSL300 from pSLJ10 & linker
RC pSL250 from pSL300
RC pSL260 from pSL250
RC pSL270 from pSL250
RC pSL251 from pSL250
RC pSL261 from pSL260
RC pSL271 from pSL270
RC pSL301 from pSL300
RC pSE1200 from pUC120 & linker
RC pSE1201 from pUC120 & linker
RC pSE1202 from pUC120 & linker
RC pSE219 from pKK233-2
RC pSE220 from pSE219
RC pSE280 from pKK421-1
RC pSE380 from pTrc99A
RA Brosius J.;
RT "Superpolylinkers in cloning and expression vectors";
RL DNA 8:759-777(1989).
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RN [2]
RC pMB9/A20 from pMB9 & zein protein A20 gene
RC pUC13/A20 from pUC13 & pMB9/A20, zein protein A20 gene
RC M13mp10/A20 from M13mp10 & pUC13/A20, zein protein A20 gene
RC M13mp321, M13mp32N from pUC13 & M13
RC M13mp321/A20 from M13mp321 & pUC13/A20
RC pZG7 from pUC9 & zein protein gene
RC M13mp18/HB-pZG7-N from M13mp18 & pZG7, zein protein gene
RC M13mp32N/ZG7, M13mp32N/BS-ZG7 from M13mp32N & M13mp18/HB-pZG7-N
RC pUC12/A20 from pUC12 & zein protein gene
RC pUC14tac from pUC12 & trp-lac promoter
RC pUC14T/A20 from pUC14tac & zein protein gene
RA Norrander J., Vieira J., Rubinstein L., Messing J.;
RT "Manipulation and expression of the maize zein storage proteins
RT in E. coli";
RL J. Biotechnol. 2:157-175(1985).
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CC MCS has all 64 possible uninterrupted palindromic hexamer sequences.
CC MCS also has 8-base restriction enzymes NotI and SfiI.
CC NM (pSL1180)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Pharmacia)
CC HO (E.coli NM522)
CC CP ()
CC FN (vector construction)(restriction site addition)
CC FN (sequencing dideoxy)(mutagenesis)
CC SE ()
CC PA (pUC118)
CC BR (pSL1190)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC118 3162bp
FT 2. SL1 superlinker 260bp
FT -> pSL1180 3422bp"
FT CDS 0..0
FT /note="REP E. coli lacI repressor gene"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(0..0)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 0..0
FT /note="ORI bacteriophage M13 intergenic region
FT (M13IG)"
FT misc_binding 0..0
FT /note="SIT unique DraIII"
FT CDS complement(0..0)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT misc_binding 0..0
FT /note="MCS HindIII-AflII-ApaLI-BsaAI-SfiI-BalI-SpeI-
FT ScaI-XhoI-SacI-AflIII-SacII-NruI-SplI-AflIII-ClaI-
FT AhaII-NarI-PvuII-PstI-NotI-EagI-NdeI-NsiI-StyI-StuI-
FT AseI-SspI-BspMII-AccI-BsaAI-NaeI-HincII-Cfr10I-KpnI-
FT XbaI-NheI-SphI-FspI-DraI-Eco47III-EcoRV-ClaI-PvuI-
FT BssHII-BglII-BspHI-BclI-BamHI-ApaI-SmaI-Bsu36I-SalI-
FT AatII-NcoI-BalI-BstBI-EcoRI"
XX
SQ Sequence 3422 BP; 873 A; 851 C; 871 G; 827 T; 0 other;
tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
gagcagattg tactgagagt gcaccataaa attgtaaacg ttaatatttt gttaaaattc
gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag
agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa
gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
gcgtactatg gttgctttga cgtatgcggt gtgaaatacc gcacagatgc gtaaggagaa
aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
ttaaggtgca cggcccacgt ggccactagt acttctcgag ctctgtacat gtccgcggtc
gcgacgtacg cgtatcgatg gcgccagctg caggcggccg ccatatgcat cctaggccta
ttaatattcc ggagtatacg tagccggcta acgttaacaa ccggtacctc tagaactata
gctagcatgc gcaaatttaa agcgctgata tcgatcgcgc gcagatctgt catgatgatc
attgcaattg gatccatata tagggcccgg gttataatta cctcaggtcg acgtcccatg
gccattcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
gc
//