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ID   PSL1190    preliminary; circular DNA; SYN; 3422 BP.
XX
AC   IG0301;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSL1190 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
XX
CC   MCS has all 64 possible uninterrupted palindromic hexamer sequences.
CC   MCS also has 8-base restriction enzymes NotI and SfiI.
CC   NM (pSL1190)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Pharmacia)
CC   HO (E.coli NM522)
CC   CP ()
CC   FN (vector construction)(restriction site addition)
CC   FN (sequencing dideoxy)(mutagenesis)
CC   SE ()
CC   PA (pUC119)
CC   BR (pSL1180)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC119 3162bp
FT                   2. SL1 superlinker 260bp
FT                   -> pSL1190 3422bp "
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13 intergenic region
FT                   (M13IG)"
FT   misc_binding    0..0
FT                   /note="SIT unique DraIII"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BstBI-BalI-NcoI-AatII-SalI-Bsu36I-
FT                   SmaI-ApaI-BamHI-BclI-BspHI-BglII-BssHII-PvuI-ClaI-
FT                   EcoRV-Eco47III-DraI-FspI-SphI-NheI-XbaI-KpnI-Cfr10I-
FT                   HincII-NaeI-BsaAI-AccI-BspMII-SspI-AseI-StuI-StyI-
FT                   NsiI-NdeI-EagI-NotI-PstI-PvuII-NarI-AhaII-ClaI-
FT                   AflIII-SplI-NruI-SacII-AflIII-SacI-XhoI-ScaI-SpeI-
FT                   BalI-SfiI-BsaAI-ApaLI-AflII-HindIII"
XX
SQ   Sequence 3422 BP; 874 A; 842 C; 880 G; 826 T; 0 other;
     tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     gagcagattg tactgagagt gcaccataaa attgtaaacg ttaatatttt gttaaaattc
     gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     ccttataaat caaaagaata gcccgagata gggttgagtg ttgttccagt ttggaacaag
     agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     gatggcccac tacgtgaacc atcacccaaa tcaagttttt tggggtcgag gtgccgtaaa
     gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     gcgtactatg gttgctttga cgtatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg
     aatggccatg ggacgtcgac ctgaggtaat tataacccgg gccctatata tggatccaat
     tgcaatgatc atcatgacag atctgcgcgc gatcgatatc agcgctttaa atttgcgcat
     gctagctata gttctagagg taccggttgt taacgttagc cggctacgta tactccggaa
     tattaatagg cctaggatgc atatggcggc cgcctgcagc tggcgccatc gatacgcgta
     cgtcgcgacc gcggacatgt acagagctcg agaagtacta gtggccacgt gggccgtgca
     ccttaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     gc
//