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ID   PSL180     preliminary; circular DNA; SYN; 2946 BP.
XX
AC   IG5130;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pSL180 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSL350 from pSE1200
RC   pSL351 from pSL350 & pUC118
RC   pKK489-7 from pSL350
RC   pKK480-3 from pSE420
RC   pKK520-3 from pKK232-8 & linker
RA   Brosius J.;
RT   "Compilation of superlinker vectors";
RL   Meth. Enzymol. 216:469-483(1992).
XX
RN   [2]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   MCS. oligonucleotide linker.
CC   NM (pSL180)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC18)(pSL1180)
CC   BR (pSL190)(pSL189)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 remove HindIII-EcoRI 51bp 400..451,
FT                   \ MCS/2635bp
FT                   2. pSL1180 HindIII-EcoRI 311bp 2878..3189,
FT                   \ SL1 superlinker
FT                   -> pSL180 2946bp"
FT   -               1..399
FT                   /note="pUC18 1..399 399bp
FT                   HindIII = A^AGCTT"
FT   -               400..710
FT                   /note="pSL1180 2878..3188 311bp
FT                   EcoRI = G^AATTC"
FT   -               711..2946
FT                   /note="pUC18 451..2686 2236bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2946 BP; 732 A; 746 C; 745 G; 723 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttaaggtg cacggcccac
     gtggccacta gtacttctcg agctctgtac atgtccgcgg tcgcgacgta cgcgtatcga
     tggcgccagc tgcaggcggc cgccatatgc atcctaggcc tattaatatt ccggagtata
     cgtagccggc taacgttaac aaccggtacc tctagaacta tagctagcat gcgcaaattt
     aaagcgctga tatcgatcgc gcgcagatct gtcatgatga tcattgcaat tggatccata
     tatagggccc gggttataat tacctcaggt cgacgtccca tggccattcg aattcgtaat
     catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     aaagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtc
//