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ID   PSL301     preliminary; circular DNA; SYN; 3264 BP.
XX
AC   IG1118;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pSL301 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pKK421-1 from pKK233-2
RC   pSLD7, pSLE1, pSLF1, pSLJ10 from pBluescript KS+
RC   pSL180 from pUC18 & linker
RC   pSL189 from pUC19 & linker
RC   pSL190 from pSL189
RC   pSL1180 from pUC118 & linker
RC   pSL1190 from pUC119 & linker
RC   pSL300 from pSLJ10 & linker
RC   pSL250 from pSL300
RC   pSL260 from pSL250
RC   pSL270 from pSL250
RC   pSL251 from pSL250
RC   pSL261 from pSL260
RC   pSL271 from pSL270
RC   pSL301 from pSL300
RC   pSE1200 from pUC120 & linker
RC   pSE1201 from pUC120 & linker
RC   pSE1202 from pUC120 & linker
RC   pSE219 from pKK233-2
RC   pSE220 from pSE219
RC   pSE280 from pKK421-1
RC   pSE380 from pTrc99A
RA   Brosius J.;
RT   "Superpolylinkers in cloning and expression vectors";
RL   DNA 8:759-777(1989).
XX
CC   NM (pSL301)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Invitrogen)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE (color blue/white)
CC   PA (pSL300 from pSLJ10 from pBluescript KS+)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript KS+ remove EcoRI-HindIII 12bp
FT                   \ 708..720, 2952bp
FT                   2. superlinker
FT                   -> pSLJ10
FT                   1. pSLJ10 remove, Bluescript linker
FT                   2. SL2 superlinker
FT                   -> pSL300 3270bp
FT                   1. pSL300 3270bp
FT                   -> pSL301 3264bp"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   promoter        0..0
FT                   /note="PRO E. coli lacO gene"
FT   RBS             0..0
FT                   /note="RBS"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS SL2 superlinker"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
XX
SQ   Sequence 3264 BP; 808 A; 821 C; 812 G; 823 T; 0 other;
     caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtacga ggacaggctg
     gagccatggc tggtgaccac gtcgtggaat gccttcgaat tcagcacctg cacatgggac
     gtcgacctga ggtaattata acccgggccc tatatatgga tccaattgca atgatcatca
     tgacagatct gcgcgcgatc gatatcagcg ctttaaattt gcgcatgcta gctatagttc
     tagaggtacc ggttgttaac gttagccggc tacgtatact ccggaatatt aataggccta
     ggatgcatat ggcggccgcc tgcagctggc gccatcgata cgcgtacgtc gcgaccgcgg
     acatgtacag agctcgagaa gtactagtgg ccacgtgggc cgtgcacctt aagcttgcct
     aatcggacga aaaaatgacc atgattacgc caagctccaa ttcgccctat agtgagtcgt
     attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     acaaaatatt aacgtttaca attt
//