Return to this vector's summary.
ID PSM17 preliminary; circular DNA; SYN; 7695 BP.
XX
AC ATCC53353;
XX
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Bacillus/Staphylococcus plasmid vector pSM17 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pSM1 from pE194 & pBR322
RC pSM3 from pSM1
RC pSM6, pSM7, pSM8 from pSM3 & linker
RC pSM100 from pUB110
RC pSM17, pSM23 from pSM100 & pSM6 & pSM7
RC pSM26 from pSM23 & linker
RC pSM27 from pSM17
RC pSM29 from pSM23
RC pSM112 from pSM29
RC pSM117 from pSM29
RC pSM115 from pSM112 & pBR322
RC pSM119 from pSM115 & pSM112
RC pSM134 from pSM112 & pLV59
RC pSM135 from pSM134 & pSM112
RA Grandi G., Del Bue M., Palla E., Mele A., Colletti E., Toma S.;
RT "New plasmid expression vectors for Bacillus subtilis";
RL Plasmid 16:1-14(1986).
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CC Created by Moore, July 1995, under contract with NCBI.
CC pBR322 sequences were removed from pSM6 (ATCC 53352) by PstI digestion
CC and replaced with pSM100 (pUB110 modified to remove the EcoRI site).
CC The EcoRI cloning site is 4 bp downstream of the ribosome binding
CC sequence.
CC Medium is -1 1065 plus kanamycin (5 ug/ml).
CC NM (pSM17)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (Bacillus subtilis SMS108)(Bacillus)(Staphylococcus aureus)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA (pUB110)(pE194)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pE194 PstI 3728bp 232..232
FT 2. pBR322 PstI 4361bp 3612..3612
FT -> pSM1 8089bp [tet in E. coli, ery in B.subtilis]
FT 1. pSM1 EcoRI 8089bp, pBR322 4360..4360
FT DNA polymerase I with AT
FT -> pSM3 8080bp [no EcoRI]
FT 1. pSM3 large HpaI-SstI/SacI 7320bp,
FT \ remove pE194 2142..2901
FT EcoRI linker 6bp gaattc:
FT 2. pE194 HaeIII 3728bp 2614..2614, 120bp 3' to ery RBS
FT exonuclease III
FT S1 nuclease, remove 120 bp from ends
FT SstI/SacI-blunt 160bp,
FT \ 156bp 5' to RBS/10bp 3' to ery RBS
FT -> pSM6 7480bp [EcoRI 4bp 3' to RBS]
FT 1. pSM3 large HpaI-SstI/SacI 7320bp,
FT \ remove pE194 2142..2901
FT EcoRI linker 6bp gaattc:
FT 2. pE194 HaeIII 3728bp 2614..2614, 120bp 3' to ery RBS
FT exonuclease III
FT S1 nuclease, remove 120 bp from ends
FT SstI/SacI-blunt 160bp,
FT \ 156bp 5' to RBS/10bp 3' to ery RBS
FT -> pSM7 7480bp [EcoRI starts in RBS]
FT 1. pUB110 EcoRI 4548bp 2..2
FT DNA polymerase I with AT
FT -> pSM100 4500bp
FT 1. pSM100 XbaI 4500bp, pUB110 465..465
FT 2. pSM6 remove PstI-PstI 3900bp,
FT \ pE194 232..232/pBR322 3614..3614/3600bp
FT XbaI 3600bp, pE194 3480..3480
FT -> pSM17 8100bp"
FT - 1..5
FT /note="pUB110 1..5 5bp
FT EcoRI = G^AATT C
FT EcoRI = G^AATTC"
FT - 6..472
FT /note="pUB110 2..468 467bp
FT XbaI = T^CTAG A
FT PstI = C TGCA^G"
FT - 473..2386
FT /note="pE194 228..2141 1914bp
FT HpaI = GTT^AAC
FT \ ttataaccctctt..."
FT - 2387..3611
FT /note="pE194 2735..3728..231 1225bp
FT PstI = CTGCA^G
FT XbaI = T^CTAGA"
FT - 3612..7695
FT /note="pUB110 465..4548 4084bp"
FT misc_binding 0..0
FT /note="SIT unique EcoRI"
FT rep_origin 0..0
FT /note="ORI Bacillus pUB110"
FT rep_origin 0..0
FT /note="ORI Staphylococcus pE194"
FT promoter 0..0
FT /note="PRO Bacillus erythromycin resistance gene
FT (ermC)"
FT CDS 0..0
FT /note="ANT Bacillus kanamycin resistance gene (kan)"
XX
SQ Sequence 7695 BP; 2489 A; 1380 C; 1272 G; 2554 T; 0 other;
gaattaattc cttagtgctt tcatagatta aactcacatc acgctttaaa tcgcttattt
tagactttaa agacttgttt tcttcaagca actcattata atcatttaca ttttcattaa
atcgctctac aagaccacta tatttttctt taacttgccc atgttcttta cttaattttt
tatattctct cgccatatca gtactcatga gatttctaac atgctgtttt aacctatcgt
tatctctcgc agcagtcact aagtttttat aatcacgctc cgatataaca acatttttgg
ttggtttctt ttctgttttc attatttctt ttcccaaacc aaacatggac ttttcacccg
ttggcacttc aacacttttc atgtgtcgtt tcgctggtac ttctaaatct gatttaactt
tatcgctata agcagtccat tcatcttttt taactgctaa atttttttct agtgcagaac
ggattgttga tgattacgaa aatattaaga gcacagacta ttacacagaa aatcaagaat
taaaaaaacg tagagagagt ttgaaagaag tagtgaatac atggaaagag gggtatcacg
aaaaaagtaa agaggttaat aaattaaagc gagagaatga tagtttgaat gagcagttga
atgtatcaga gaaatttcaa gctagtacag tgactttata tcgtgctgcg agggcgaatt
tccctgggtt tgagaaaggg tttaataggc ttaaagagaa attctttaat gattccaaat
ttgagcgtgt gggacagttt atggatgttg tacaggataa tgtccagaag gtcgatagaa
agcgtgagaa acagcgtaca gacgatttag agatgtagag gtacttttat gccgagaaaa
ctttttgcgt gtgacagtcc ttaaaatata cttagagcgt aagcgaaagt agtagcgaca
gctattaact ttcggttgca aagctctagg atttttaatg gacgcagcgc atcacacgca
aaaaggaaat tggaataaat gcgaaatttg agatgttaat taaagacctt tttgaggtct
ttttttctta gatttttggg gttatttagg ggagaaaaca taggggggta ctacgacctc
ccccctaggt gtccattgtc cattgtccaa acaaataaat aaatattggg tttttaatgt
taaaaggttg ttttttatgt taaagtgaaa aaaacagatg ttgggaggta cagtgatggt
tgtagataga aaagaagaga aaaaagttgc tgttacttta agacttacaa cagaagaaaa
tgagatatta aatagaatca aagaaaaata taatattagc aaatcagatg caaccggtat
tctaataaaa aaatatgcaa aggaggaata cggtgcattt taaacaaaaa aagatagaca
gcactggcat gctgcctatc tatgactaaa ttttgttaag tgtattagca ccgttattat
atcatgagcg aaaatgtaat aaaagaaact gaaaacaaga aaaattcaag aggacgtaat
tggacatttg ttttatatcc agaatcagca aaagccgagt ggttagagta tttaaaagag
ttacacattc aatttgtagt gtctccatta catgataggg atactgatac agaaggtagg
atgaaaaaag agcattatca tattctagtg atgtatgagg gtaataaatc ttatgaacag
ataaaaataa ttaacagaag aattgaatgc gactattccg cagattgcag gaagtgtgaa
aggtcttgtg agatatatgc ttcacatgga cgatcctaat aaatttaaat atcaaaaaga
agatatgata gtttatggcg gtgtagatgt tgatgaatta ttaaagaaaa caacaacaga
tagatataaa ttaattaaag aaatgattga gtttattgat gaacaaggaa tcgtagaatt
taagagttta atggattatg caatgaagtt taaatttgat gattggttcc cgcttttatg
tgataactcg gcgtatgtta ttcaagaata tataaaatca aatcggtata aatctgaccg
atagattttg aatttaggtg tcacaagaca ctcttttttc gcaccagcga aaactggttt
aagccgactg cgcaaaagac ataatcgatt cacaaaaaat aggcacacga aaaacaagtt
aagggatgca gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa
gagataagaa ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg
aattgttaaa ttgatttttt gtaaatattt tcttgtattc tttgtttcat tataaccctc
tttaatttgg ttatatgaat tttgcttatt aacgattcat tataaccact tattttttgt
ttggttgata atgaactgtg ctgattacaa aaatactaaa aatgcccata ttttttcctc
cttataaaat tagtataatt atagcacgag ctctgataaa tatgaacatg atgagtgatc
gttaaattta tactgcaatc ggatgcgatt attgaataaa agatatgaga gatttatcta
atttcttttt tcttgtaaaa aaagaaagtt cttaaaggtt ttatagtttt ggtcgtagag
cacacggttt aacgacttaa ttacgaagta aataagtcta gtgtgttaga ctttatgaaa
tctatatacg tttatatata tttattatcc ggaggtgtag catgtctcat tcaattttga
gggttgccag agttaaagga tcaagtaata caaacgggat acaaagacat aatcaaagag
agaataaaaa ctataataat aaagacataa atcatgagga aacatataaa aattatgatt
tgattaacgc acaaaatata aagtataaag ataaaattga tgaaacgatt gatgagaatt
attcagggaa acgtaaaatt cggtcagatg caattcgaca tgtggacgga ctggttacaa
gtgataaaga tttctttgat gatttaagcg gagaagaaat agaacgattt tttaaagata
gcttggagtt tctagaaaat gaatacggta aggaaaatat gctgtatgcg actgtccatc
tggatgaaag agtcccacat atgcactttg gttttgtccc tttaacagag gacgggagat
tgtctgcaaa agaacagtta ggcaacaaga aagactttac tcaattacaa gatagattta
atgagtatgt gaatgagaaa ggttatgaac ttgaaagagg cacgtccaaa gaggttacag
aacgagaaca taaagcgatg gatcagtaca agaaagatac tgtatttcat aaacaggaac
tgcaagaagt taaggatgag ttacagaagg caaataagca gttacagagt ggaatagagc
atatgaggtc tacgaaaccc tttgattatg aaaatgagcg tacaggtttg ttctctggac
gtgaagagac tggtagaaag atattaactg ctgatgaatt tgaacgcctg caagaaacaa
tctcttctgc actagaaaat caatctcttt ttccaaagtt tgttttttaa atttagctgt
ctcaatatgt ttacggtcag agccacgttc accacgcttc aactcaaaac cctgtttttt
catatgctcg gggaatttat cttgtagcca taacagttct tgacgattaa acacattttt
tccttgcagt tttccatcac gcataggcac aacacctaaa tgcatgtgag gggtttgctc
atcattatga actgttgcat aagcaatatt ttgcttgcca tatcgttcgg aaaataattt
ataactttcc tcaaaaaatc gtttttgttc tcctggatcc agttgctcaa aaaaatctcg
gtcagatgtt actagcaact catttacaag aacagcatct ttcctccgtt tttcttgtac
ctgttttttg tgattcaata atttctttga cacgttcgtt gtaatcaata tttttatcat
ttttcaaatc ataattttca cgtgttcgct catggtcaat atcatcattc gttctacttt
ttcgctctct ttgattatga aattgcatgc cttttagtcc agctgatttc actttttgca
ttctacaaac tgcataactc atatgtaaat cgctcctttt taggtggcac aaatgtgagg
cattttcgct ctttccggca accacttcca agtaaagtat aacacactat actttatatt
cataaagtgt gtgctctgcg aggctgtcgg cagtgccgac caaaaccata aaacctttaa
gacctttctt ttttttacga gaaaaaagaa acaaaaaaac ctgccctctg ccacctcagc
aaaggggggt tttgctctcg tgctcgttta aaaatcagca agggacaggt agtatttttt
gagaagatca ctcaaaaaat ctccaccttt aaacccttgc caatttttat tttgtccgtt
ttgtctagct taccgaaagc cagactcagc aagaataaaa tttttattgt ctttcggttt
tctagtgtaa cggacaaaac cactcaaaat aaaaaagata caagagaggt ctctcgtatc
ttttattcag caatcgcgcc cgattgctga acagattaat aatagatttt agctttttat
ttgttgaaaa aagctaatca aattgttgtc gggatcaatt actgcaaagt ctcgttcatc
ccaccactga tcttttaatg atgtattggg gtgcaaaatg cccaaaggct taatatgttg
atataattca tcaattccct ctacttcaat gcggcaacta gcagtaccag caataaacga
ctccgcacct gtacaaaccg gtgaatcatt actacgagag cgccagcttc atcacttgcc
tcccatagat gaatccgaac ctcattacac attagaactg cgaatccatc ttcatggtga
accaaagtga aacctagttt atcgcaataa aaacctatac tctttttaat atccccgact
ggcaatgccg ggatagactg taacattctc acgcataaaa tcccctttca ttttctaatg
taaatctatt accttattat taattcaatt cgctcataat taatcctttt tcttattacg
caaaatggcc cgatttaagc acacccttta ttccgttaat gcgccatgac agccatgata
attactaata ctaggagaag ttaataaata cgtaaccaac atgattaaca attattagag
gtcatcgttc aaaatggtat gcgttttgac acatccacta tatatccgtg tcgttctgtc
cactcctgaa tcccattcca gaaattctct agcgattcca gaagtttctc agagtcggaa
agttgaccag acattacgaa ctggcacaga tggtcataac ctgaaggaag atctgattgc
ttaactgctt cagttaagac cgaagcgctc gtcgtataac agatgcgatg atgcagacca
atcaacatgg cacctgccat tgctacctgt acagtcaagg atggtagaaa tgttgtcggt
ccttgcacac gaatattacg ccatttgcct gcatattcaa acagctcttc tacgataagg
gcacaaatcg catcgtggaa cgtttgggct tctaccgatt tagcagtttg atacactttc
tctaagtatc cacctgaatc ataaatcggc aaaatagaga aaaattgacc atgtgtaagc
ggccaatctg attccacctg agatgcataa tctagtagaa tctcttcgct atcaaaattc
acttccacct tccactcacc ggttgtccat tcatggctga actctgcttc ctctgttgac
atgacacaca tcatctcaat atccgaatag ggcccatcag tctgacgacc aagagagcca
taaacaccaa tagccttaac atcatcccca tatttatcca atattcgttc cttaatttca
tgaacaatct tcattctttc ttctctagtc attattattg gtccattcac tattctcatt
cccttttcag ataattttag atttgctttt ctaaataaga atatttggag agcaccgttc
ttattcagct attaataact cgtcttccta agcatccttc aatcctttta ataacaatta
tagcatctaa tcttcaacaa actggcccgt ttgttgaact actctttaat aaaataattt
ttccgttccc aattccacat tgcaataata gaaaatccat cttcatcggc tttttcgtca
tcatctgtat gaatcaaatc gccttcttct gtgtcatcaa ggtttaattt tttatgtatt
tcttttaaca aaccaccata ggagattaac cttttacggt gtaaaccttc ctccaaatca
gacaaacgtt tcaaattctt ttcttcatca tcggtcataa aatccgtatc ctttacagga
tattttgcag tttcgtcaat tgccgattgt atatccgatt tatatttatt tttcggtcga
atcatttgaa cttttacatt tggatcatag tctaatttca ttgccttttt ccaaaattga
atccattgtt tttgattcac gtagttttct gtattcttaa aataagttgg ttccacacat
accaatacat gcatgtgctg attataagaa ttatctttat tatttattgt cacttccgtt
gcacgcataa aaccaacaag atttttatta atttttttat attgcatcat tcggcgaaat
ccttgagcca tatctgacaa actcttattt aattcttcgc catcataaac atttttaact
gttaatgtga gaaacaacca acgaactgtt ggcttttgtt taataacttc agcaacaacc
ttttgtgact gaatgccatg tttcattgct ctcctccagt tgcacattgg acaaagcctg
gatttacaaa accacactcg atacaacttt ctttcgcctg tttcacgatt ttgtttatac
tctaatattt cagcacaatc ttttactctt tcagcctttt taaattcaag aatatgcaga
agttcaaagt aatcaacatt agcgattttc ttttctctcc atggtctcac ttttccactt
tttgtcttgt ccactaaaac ccttgatttt tcatctgaat aaatgctact attaggacac
ataatattaa aagaaacccc catctattta gttatttgtt tagtcactta taactttaac
agatggggtt tttctgtgca accaatttta agggttttca atactttaaa acacatacat
accaacactt caacgcacct ttcagcaact aaaataaaaa tgacgttatt tctatatgta
tcaagataag aaagaacaag ttcaaaacca tcaaaaaaag acaccttttc aggtgctttt
tttattttat aaactcattc cctgatctcg acttcgttct ttttttacct ctcggttatg
agttagttca aattcgttct ttttaggttc taaatcgtgt ttttcttgga attgtgctgt
tttatccttt accttgtcta caaacccctt aaaaacgttt ttaaaggctt ttaagccgtc
tgtacgttcc ttaag
//