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ID   PSM17      preliminary; circular DNA; SYN; 7695 BP.
XX
AC   ATCC53353;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/Staphylococcus plasmid vector pSM17 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSM1 from pE194 & pBR322
RC   pSM3 from pSM1
RC   pSM6, pSM7, pSM8 from pSM3 & linker
RC   pSM100 from pUB110
RC   pSM17, pSM23 from pSM100 & pSM6 & pSM7
RC   pSM26 from pSM23 & linker
RC   pSM27 from pSM17
RC   pSM29 from pSM23
RC   pSM112 from pSM29
RC   pSM117 from pSM29
RC   pSM115 from pSM112 & pBR322
RC   pSM119 from pSM115 & pSM112
RC   pSM134 from pSM112 & pLV59
RC   pSM135 from pSM134 & pSM112
RA   Grandi G., Del Bue M., Palla E., Mele A., Colletti E., Toma S.;
RT   "New plasmid expression vectors for Bacillus subtilis";
RL   Plasmid 16:1-14(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   pBR322 sequences were removed from pSM6 (ATCC 53352) by PstI digestion
CC   and replaced with pSM100 (pUB110 modified to remove the EcoRI site).
CC   The EcoRI cloning site is 4 bp downstream of the ribosome binding
CC   sequence.
CC   Medium is -1 1065 plus kanamycin (5 ug/ml).
CC   NM (pSM17)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis SMS108)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUB110)(pE194)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pE194 PstI 3728bp 232..232
FT                   2. pBR322 PstI 4361bp 3612..3612
FT                   -> pSM1 8089bp [tet in E. coli, ery in B.subtilis]
FT                   1. pSM1 EcoRI 8089bp, pBR322 4360..4360
FT                   DNA polymerase I with AT
FT                   -> pSM3 8080bp [no EcoRI]
FT                   1. pSM3 large HpaI-SstI/SacI 7320bp,
FT                   \ remove pE194 2142..2901
FT                   EcoRI linker 6bp gaattc:
FT                   2. pE194 HaeIII 3728bp 2614..2614, 120bp 3' to ery RBS
FT                   exonuclease III
FT                   S1 nuclease, remove 120 bp from ends
FT                   SstI/SacI-blunt 160bp,
FT                   \ 156bp 5' to RBS/10bp 3' to ery RBS
FT                   -> pSM6 7480bp [EcoRI 4bp 3' to RBS]
FT                   1. pSM3 large HpaI-SstI/SacI 7320bp,
FT                   \ remove pE194 2142..2901
FT                   EcoRI linker 6bp gaattc:
FT                   2. pE194 HaeIII 3728bp 2614..2614, 120bp 3' to ery RBS
FT                   exonuclease III
FT                   S1 nuclease, remove 120 bp from ends
FT                   SstI/SacI-blunt 160bp,
FT                   \ 156bp 5' to RBS/10bp 3' to ery RBS
FT                   -> pSM7 7480bp [EcoRI starts in RBS]
FT                   1. pUB110 EcoRI 4548bp 2..2
FT                   DNA polymerase I with AT
FT                   -> pSM100 4500bp
FT                   1. pSM100 XbaI 4500bp, pUB110 465..465
FT                   2. pSM6 remove PstI-PstI 3900bp,
FT                   \ pE194 232..232/pBR322 3614..3614/3600bp
FT                   XbaI 3600bp, pE194 3480..3480
FT                   -> pSM17 8100bp"
FT   -               1..5
FT                   /note="pUB110 1..5 5bp
FT                   EcoRI = G^AATT C
FT                   EcoRI =      G^AATTC"
FT   -               6..472
FT                   /note="pUB110 2..468 467bp
FT                   XbaI = T^CTAG A
FT                   PstI =      C TGCA^G"
FT   -               473..2386
FT                   /note="pE194 228..2141 1914bp
FT                   HpaI = GTT^AAC
FT                   \              ttataaccctctt..."
FT   -               2387..3611
FT                   /note="pE194 2735..3728..231 1225bp
FT                   PstI = CTGCA^G
FT                   XbaI =     T^CTAGA"
FT   -               3612..7695
FT                   /note="pUB110 465..4548 4084bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pUB110"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pE194"
FT   promoter        0..0
FT                   /note="PRO Bacillus erythromycin resistance gene
FT                   (ermC)"
FT   CDS             0..0
FT                   /note="ANT Bacillus kanamycin resistance gene (kan)"
XX
SQ   Sequence 7695 BP; 2489 A; 1380 C; 1272 G; 2554 T; 0 other;
     gaattaattc cttagtgctt tcatagatta aactcacatc acgctttaaa tcgcttattt
     tagactttaa agacttgttt tcttcaagca actcattata atcatttaca ttttcattaa
     atcgctctac aagaccacta tatttttctt taacttgccc atgttcttta cttaattttt
     tatattctct cgccatatca gtactcatga gatttctaac atgctgtttt aacctatcgt
     tatctctcgc agcagtcact aagtttttat aatcacgctc cgatataaca acatttttgg
     ttggtttctt ttctgttttc attatttctt ttcccaaacc aaacatggac ttttcacccg
     ttggcacttc aacacttttc atgtgtcgtt tcgctggtac ttctaaatct gatttaactt
     tatcgctata agcagtccat tcatcttttt taactgctaa atttttttct agtgcagaac
     ggattgttga tgattacgaa aatattaaga gcacagacta ttacacagaa aatcaagaat
     taaaaaaacg tagagagagt ttgaaagaag tagtgaatac atggaaagag gggtatcacg
     aaaaaagtaa agaggttaat aaattaaagc gagagaatga tagtttgaat gagcagttga
     atgtatcaga gaaatttcaa gctagtacag tgactttata tcgtgctgcg agggcgaatt
     tccctgggtt tgagaaaggg tttaataggc ttaaagagaa attctttaat gattccaaat
     ttgagcgtgt gggacagttt atggatgttg tacaggataa tgtccagaag gtcgatagaa
     agcgtgagaa acagcgtaca gacgatttag agatgtagag gtacttttat gccgagaaaa
     ctttttgcgt gtgacagtcc ttaaaatata cttagagcgt aagcgaaagt agtagcgaca
     gctattaact ttcggttgca aagctctagg atttttaatg gacgcagcgc atcacacgca
     aaaaggaaat tggaataaat gcgaaatttg agatgttaat taaagacctt tttgaggtct
     ttttttctta gatttttggg gttatttagg ggagaaaaca taggggggta ctacgacctc
     ccccctaggt gtccattgtc cattgtccaa acaaataaat aaatattggg tttttaatgt
     taaaaggttg ttttttatgt taaagtgaaa aaaacagatg ttgggaggta cagtgatggt
     tgtagataga aaagaagaga aaaaagttgc tgttacttta agacttacaa cagaagaaaa
     tgagatatta aatagaatca aagaaaaata taatattagc aaatcagatg caaccggtat
     tctaataaaa aaatatgcaa aggaggaata cggtgcattt taaacaaaaa aagatagaca
     gcactggcat gctgcctatc tatgactaaa ttttgttaag tgtattagca ccgttattat
     atcatgagcg aaaatgtaat aaaagaaact gaaaacaaga aaaattcaag aggacgtaat
     tggacatttg ttttatatcc agaatcagca aaagccgagt ggttagagta tttaaaagag
     ttacacattc aatttgtagt gtctccatta catgataggg atactgatac agaaggtagg
     atgaaaaaag agcattatca tattctagtg atgtatgagg gtaataaatc ttatgaacag
     ataaaaataa ttaacagaag aattgaatgc gactattccg cagattgcag gaagtgtgaa
     aggtcttgtg agatatatgc ttcacatgga cgatcctaat aaatttaaat atcaaaaaga
     agatatgata gtttatggcg gtgtagatgt tgatgaatta ttaaagaaaa caacaacaga
     tagatataaa ttaattaaag aaatgattga gtttattgat gaacaaggaa tcgtagaatt
     taagagttta atggattatg caatgaagtt taaatttgat gattggttcc cgcttttatg
     tgataactcg gcgtatgtta ttcaagaata tataaaatca aatcggtata aatctgaccg
     atagattttg aatttaggtg tcacaagaca ctcttttttc gcaccagcga aaactggttt
     aagccgactg cgcaaaagac ataatcgatt cacaaaaaat aggcacacga aaaacaagtt
     aagggatgca gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa
     gagataagaa ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg
     aattgttaaa ttgatttttt gtaaatattt tcttgtattc tttgtttcat tataaccctc
     tttaatttgg ttatatgaat tttgcttatt aacgattcat tataaccact tattttttgt
     ttggttgata atgaactgtg ctgattacaa aaatactaaa aatgcccata ttttttcctc
     cttataaaat tagtataatt atagcacgag ctctgataaa tatgaacatg atgagtgatc
     gttaaattta tactgcaatc ggatgcgatt attgaataaa agatatgaga gatttatcta
     atttcttttt tcttgtaaaa aaagaaagtt cttaaaggtt ttatagtttt ggtcgtagag
     cacacggttt aacgacttaa ttacgaagta aataagtcta gtgtgttaga ctttatgaaa
     tctatatacg tttatatata tttattatcc ggaggtgtag catgtctcat tcaattttga
     gggttgccag agttaaagga tcaagtaata caaacgggat acaaagacat aatcaaagag
     agaataaaaa ctataataat aaagacataa atcatgagga aacatataaa aattatgatt
     tgattaacgc acaaaatata aagtataaag ataaaattga tgaaacgatt gatgagaatt
     attcagggaa acgtaaaatt cggtcagatg caattcgaca tgtggacgga ctggttacaa
     gtgataaaga tttctttgat gatttaagcg gagaagaaat agaacgattt tttaaagata
     gcttggagtt tctagaaaat gaatacggta aggaaaatat gctgtatgcg actgtccatc
     tggatgaaag agtcccacat atgcactttg gttttgtccc tttaacagag gacgggagat
     tgtctgcaaa agaacagtta ggcaacaaga aagactttac tcaattacaa gatagattta
     atgagtatgt gaatgagaaa ggttatgaac ttgaaagagg cacgtccaaa gaggttacag
     aacgagaaca taaagcgatg gatcagtaca agaaagatac tgtatttcat aaacaggaac
     tgcaagaagt taaggatgag ttacagaagg caaataagca gttacagagt ggaatagagc
     atatgaggtc tacgaaaccc tttgattatg aaaatgagcg tacaggtttg ttctctggac
     gtgaagagac tggtagaaag atattaactg ctgatgaatt tgaacgcctg caagaaacaa
     tctcttctgc actagaaaat caatctcttt ttccaaagtt tgttttttaa atttagctgt
     ctcaatatgt ttacggtcag agccacgttc accacgcttc aactcaaaac cctgtttttt
     catatgctcg gggaatttat cttgtagcca taacagttct tgacgattaa acacattttt
     tccttgcagt tttccatcac gcataggcac aacacctaaa tgcatgtgag gggtttgctc
     atcattatga actgttgcat aagcaatatt ttgcttgcca tatcgttcgg aaaataattt
     ataactttcc tcaaaaaatc gtttttgttc tcctggatcc agttgctcaa aaaaatctcg
     gtcagatgtt actagcaact catttacaag aacagcatct ttcctccgtt tttcttgtac
     ctgttttttg tgattcaata atttctttga cacgttcgtt gtaatcaata tttttatcat
     ttttcaaatc ataattttca cgtgttcgct catggtcaat atcatcattc gttctacttt
     ttcgctctct ttgattatga aattgcatgc cttttagtcc agctgatttc actttttgca
     ttctacaaac tgcataactc atatgtaaat cgctcctttt taggtggcac aaatgtgagg
     cattttcgct ctttccggca accacttcca agtaaagtat aacacactat actttatatt
     cataaagtgt gtgctctgcg aggctgtcgg cagtgccgac caaaaccata aaacctttaa
     gacctttctt ttttttacga gaaaaaagaa acaaaaaaac ctgccctctg ccacctcagc
     aaaggggggt tttgctctcg tgctcgttta aaaatcagca agggacaggt agtatttttt
     gagaagatca ctcaaaaaat ctccaccttt aaacccttgc caatttttat tttgtccgtt
     ttgtctagct taccgaaagc cagactcagc aagaataaaa tttttattgt ctttcggttt
     tctagtgtaa cggacaaaac cactcaaaat aaaaaagata caagagaggt ctctcgtatc
     ttttattcag caatcgcgcc cgattgctga acagattaat aatagatttt agctttttat
     ttgttgaaaa aagctaatca aattgttgtc gggatcaatt actgcaaagt ctcgttcatc
     ccaccactga tcttttaatg atgtattggg gtgcaaaatg cccaaaggct taatatgttg
     atataattca tcaattccct ctacttcaat gcggcaacta gcagtaccag caataaacga
     ctccgcacct gtacaaaccg gtgaatcatt actacgagag cgccagcttc atcacttgcc
     tcccatagat gaatccgaac ctcattacac attagaactg cgaatccatc ttcatggtga
     accaaagtga aacctagttt atcgcaataa aaacctatac tctttttaat atccccgact
     ggcaatgccg ggatagactg taacattctc acgcataaaa tcccctttca ttttctaatg
     taaatctatt accttattat taattcaatt cgctcataat taatcctttt tcttattacg
     caaaatggcc cgatttaagc acacccttta ttccgttaat gcgccatgac agccatgata
     attactaata ctaggagaag ttaataaata cgtaaccaac atgattaaca attattagag
     gtcatcgttc aaaatggtat gcgttttgac acatccacta tatatccgtg tcgttctgtc
     cactcctgaa tcccattcca gaaattctct agcgattcca gaagtttctc agagtcggaa
     agttgaccag acattacgaa ctggcacaga tggtcataac ctgaaggaag atctgattgc
     ttaactgctt cagttaagac cgaagcgctc gtcgtataac agatgcgatg atgcagacca
     atcaacatgg cacctgccat tgctacctgt acagtcaagg atggtagaaa tgttgtcggt
     ccttgcacac gaatattacg ccatttgcct gcatattcaa acagctcttc tacgataagg
     gcacaaatcg catcgtggaa cgtttgggct tctaccgatt tagcagtttg atacactttc
     tctaagtatc cacctgaatc ataaatcggc aaaatagaga aaaattgacc atgtgtaagc
     ggccaatctg attccacctg agatgcataa tctagtagaa tctcttcgct atcaaaattc
     acttccacct tccactcacc ggttgtccat tcatggctga actctgcttc ctctgttgac
     atgacacaca tcatctcaat atccgaatag ggcccatcag tctgacgacc aagagagcca
     taaacaccaa tagccttaac atcatcccca tatttatcca atattcgttc cttaatttca
     tgaacaatct tcattctttc ttctctagtc attattattg gtccattcac tattctcatt
     cccttttcag ataattttag atttgctttt ctaaataaga atatttggag agcaccgttc
     ttattcagct attaataact cgtcttccta agcatccttc aatcctttta ataacaatta
     tagcatctaa tcttcaacaa actggcccgt ttgttgaact actctttaat aaaataattt
     ttccgttccc aattccacat tgcaataata gaaaatccat cttcatcggc tttttcgtca
     tcatctgtat gaatcaaatc gccttcttct gtgtcatcaa ggtttaattt tttatgtatt
     tcttttaaca aaccaccata ggagattaac cttttacggt gtaaaccttc ctccaaatca
     gacaaacgtt tcaaattctt ttcttcatca tcggtcataa aatccgtatc ctttacagga
     tattttgcag tttcgtcaat tgccgattgt atatccgatt tatatttatt tttcggtcga
     atcatttgaa cttttacatt tggatcatag tctaatttca ttgccttttt ccaaaattga
     atccattgtt tttgattcac gtagttttct gtattcttaa aataagttgg ttccacacat
     accaatacat gcatgtgctg attataagaa ttatctttat tatttattgt cacttccgtt
     gcacgcataa aaccaacaag atttttatta atttttttat attgcatcat tcggcgaaat
     ccttgagcca tatctgacaa actcttattt aattcttcgc catcataaac atttttaact
     gttaatgtga gaaacaacca acgaactgtt ggcttttgtt taataacttc agcaacaacc
     ttttgtgact gaatgccatg tttcattgct ctcctccagt tgcacattgg acaaagcctg
     gatttacaaa accacactcg atacaacttt ctttcgcctg tttcacgatt ttgtttatac
     tctaatattt cagcacaatc ttttactctt tcagcctttt taaattcaag aatatgcaga
     agttcaaagt aatcaacatt agcgattttc ttttctctcc atggtctcac ttttccactt
     tttgtcttgt ccactaaaac ccttgatttt tcatctgaat aaatgctact attaggacac
     ataatattaa aagaaacccc catctattta gttatttgtt tagtcactta taactttaac
     agatggggtt tttctgtgca accaatttta agggttttca atactttaaa acacatacat
     accaacactt caacgcacct ttcagcaact aaaataaaaa tgacgttatt tctatatgta
     tcaagataag aaagaacaag ttcaaaacca tcaaaaaaag acaccttttc aggtgctttt
     tttattttat aaactcattc cctgatctcg acttcgttct ttttttacct ctcggttatg
     agttagttca aattcgttct ttttaggttc taaatcgtgt ttttcttgga attgtgctgt
     tttatccttt accttgtcta caaacccctt aaaaacgttt ttaaaggctt ttaagccgtc
     tgtacgttcc ttaag
//