back Return to this vector's summary.
ID   PSM6       preliminary; circular DNA; SYN; 7503 BP.
XX
AC   ATCC53352;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/E.coli plasmid vector pSM6 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSM1 from pE194 & pBR322
RC   pSM3 from pSM1
RC   pSM6, pSM7, pSM8 from pSM3 & linker
RC   pSM100 from pUB110
RC   pSM17, pSM23 from pSM100 & pSM6 & pSM7
RC   pSM26 from pSM23 & linker
RC   pSM27 from pSM17
RC   pSM29 from pSM23
RC   pSM112 from pSM29
RC   pSM117 from pSM29
RC   pSM115 from pSM112 & pBR322
RC   pSM119 from pSM115 & pSM112
RC   pSM134 from pSM112 & pLV59
RC   pSM135 from pSM134 & pSM112
RA   Grandi G., Del Bue M., Palla E., Mele A., Colletti E., Toma S.;
RT   "New plasmid expression vectors for Bacillus subtilis";
RL   Plasmid 16:1-14(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Constructed by ligating pBR322 and pE194 after linearizing with PstI.
CC   The EcoRI site was destroyed and a HpaI/SstI fragment containing ErmC
CC   was removed.  The ErmC promoter and ribosome binding sequence was
CC   inserted upstream of an EcoRI linker.
CC   The EcoRI cloning site is 4 bp downstream of the ribosome binding
CC   sequence.
CC   pSM6 can be maintained in both E.coli and B. subtilis but can be used
CC   as a cloning vector only in E.coli because the tetracycline resistance
CC   is poorly expressed in B. subtilis.
CC   Medium is 1273 LB plus tetracycline.
CC   NM (pSM6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pE194)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pE194 PstI 3728bp 232..232
FT                   2. pBR322 PstI 4361bp 3612..3612
FT                   -> pSM1 8089bp [tet in E. coli, ery in B.subtilis]
FT                   1. pSM1 EcoRI 8089bp, pBR322 4360..4360
FT                   DNA polymerase I with AT
FT                   -> pSM3 8093bp [no EcoRI]
FT                   1. pSM3 large HpaI-SstI/SacI 7320bp,
FT                   \ remove pE194 2142..2901
FT                   EcoRI linker 6bp gaattc:
FT                   2. pE194 HaeIII 3728bp 2614..2614, 120bp 3' to ery RBS
FT                   exonuclease III
FT                   S1 nuclease, remove 120 bp from ends
FT                   blunt-SstI/SacI 163bp 2738..2901,
FT                   \ pE194 SacI 2901
FT                   \ 156bp 5' to RBS/10bp 3' to ery RBS
FT                   -> pSM6 8000bp [EcoRI 4bp 3' to RBS]"
FT   -               1..5521
FT                   /note="pSM3 1..5521 5521bp
FT                   \ 232..2141/2901..3728..231
FT                   HpaI =  GTT^AAC
FT                   \           gaattc"
FT   -               5522..5527
FT                   /note="gaattc 6bp
FT                   \        gaattc
FT                   \              ttataaccctctt..."
FT   -               5528..5690
FT                   /note="pE194 2738..2900 163bp
FT                   SacI = SstI = GAGCT^C"
FT   -               5691..7503
FT                   /note="pSM3 6281..8093 1813bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pE194"
FT   promoter        0..0
FT                   /note="PRO E. coli ermC gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 7503 BP; 2164 A; 1588 C; 1781 G; 1970 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc agaacggatt gttgatgatt acgaaaatat taagagcaca gactattaca
     cagaaaatca agaattaaaa aaacgtagag agagtttgaa agaagtagtg aatacatgga
     aagaggggta tcacgaaaaa agtaaagagg ttaataaatt aaagcgagag aatgatagtt
     tgaatgagca gttgaatgta tcagagaaat ttcaagctag tacagtgact ttatatcgtg
     ctgcgagggc gaatttccct gggtttgaga aagggtttaa taggcttaaa gagaaattct
     ttaatgattc caaatttgag cgtgtgggac agtttatgga tgttgtacag gataatgtcc
     agaaggtcga tagaaagcgt gagaaacagc gtacagacga tttagagatg tagaggtact
     tttatgccga gaaaactttt tgcgtgtgac agtccttaaa atatacttag agcgtaagcg
     aaagtagtag cgacagctat taactttcgg ttgcaaagct ctaggatttt taatggacgc
     agcgcatcac acgcaaaaag gaaattggaa taaatgcgaa atttgagatg ttaattaaag
     acctttttga ggtctttttt tcttagattt ttggggttat ttaggggaga aaacataggg
     gggtactacg acctcccccc taggtgtcca ttgtccattg tccaaacaaa taaataaata
     ttgggttttt aatgttaaaa ggttgttttt tatgttaaag tgaaaaaaac agatgttggg
     aggtacagtg atggttgtag atagaaaaga agagaaaaaa gttgctgtta ctttaagact
     tacaacagaa gaaaatgaga tattaaatag aatcaaagaa aaatataata ttagcaaatc
     agatgcaacc ggtattctaa taaaaaaata tgcaaaggag gaatacggtg cattttaaac
     aaaaaaagat agacagcact ggcatgctgc ctatctatga ctaaattttg ttaagtgtat
     tagcaccgtt attatatcat gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat
     tcaagaggac gtaattggac atttgtttta tatccagaat cagcaaaagc cgagtggtta
     gagtatttaa aagagttaca cattcaattt gtagtgtctc cattacatga tagggatact
     gatacagaag gtaggatgaa aaaagagcat tatcatattc tagtgatgta tgagggtaat
     aaatcttatg aacagataaa aataattaac agaagaattg aatgcgacta ttccgcagat
     tgcaggaagt gtgaaaggtc ttgtgagata tatgcttcac atggacgatc ctaataaatt
     taaatatcaa aaagaagata tgatagttta tggcggtgta gatgttgatg aattattaaa
     gaaaacaaca acagatagat ataaattaat taaagaaatg attgagttta ttgatgaaca
     aggaatcgta gaatttaaga gtttaatgga ttatgcaatg aagtttaaat ttgatgattg
     gttcccgctt ttatgtgata actcggcgta tgttattcaa gaatatataa aatcaaatcg
     gtataaatct gaccgataga ttttgaattt aggtgtcaca agacactctt ttttcgcacc
     agcgaaaact ggtttaagcc gactgcgcaa aagacataat cgattcacaa aaaataggca
     cacgaaaaac aagttaaggg atgcagttta tgcatccctt aacttactta ttaaataatt
     tatagctatt gaaaagagat aagaattgtt caaagctaat attgtttaaa tcgtcaattc
     ctgcatgttt taaggaattg ttaaattgat tttttgtaaa tattttcttg tattctttgt
     tgaattctta taaccctctt taatttggtt atatgaattt tgcttattaa cgattcatta
     taaccactta ttttttgttt ggttgataat gaactgtgct gattacaaaa atactaaaaa
     tgcccatatt ttttcctcct tataaaatta gtataattat agcacgagct ctgataaata
     tgaacatgat gagtgatcgt taaatttata ctgcaatcgg atgcgattat tgaataaaag
     atatgagaga tttatctaat ttcttttttc ttgtaaaaaa agaaagttct taaaggtttt
     atagttttgg tcgtagagca cacggtttaa cgacttaatt acgaagtaaa taagtctagt
     gtgttagact ttatgaaatc tatatacgtt tatatatatt tattatccgg aggtgtagca
     tgtctcattc aattttgagg gttgccagag ttaaaggatc aagtaataca aacgggatac
     aaagacataa tcaaagagag aataaaaact ataataataa agacataaat catgaggaaa
     catataaaaa ttatgatttg attaacgcac aaaatataaa gtataaagat aaaattgatg
     aaacgattga tgagaattat tcagggaaac gtaaaattcg gtcagatgca attcgacatg
     tggacggact ggttacaagt gataaagatt tctttgatga tttaagcgga gaagaaatag
     aacgattttt taaagatagc ttggagtttc tagaaaatga atacggtaag gaaaatatgc
     tgtatgcgac tgtccatctg gatgaaagag tcccacatat gcactttggt tttgtccctt
     taacagagga cgggagattg tctgcaaaag aacagttagg caacaagaaa gactttactc
     aattacaaga tagatttaat gagtatgtga atgagaaagg ttatgaactt gaaagaggca
     cgtccaaaga ggttacagaa cgagaacata aagcgatgga tcagtacaag aaagatactg
     tatttcataa acaggaactg caagaagtta aggatgagtt acagaaggca aataagcagt
     tacagagtgg aatagagcat atgaggtcta cgaaaccctt tgattatgaa aatgagcgta
     caggtttgtt ctctggacgt gaagagactg gtagaaagat attaactgct gatgaatttg
     aacgcctgca agaaacaatc tcttctgcag gcatcgtggt gtcacgctcg tcgtttggta
     tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt
     taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat
     taa
//