back Return to this vector's summary.
ID   PSM7       preliminary; circular DNA; SYN; 7500 BP.
XX
AC   IG9804;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Bacillus/E.coli plasmid vector pSM7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSM1 from pE194 & pBR322
RC   pSM3 from pSM1
RC   pSM6, pSM7, pSM8 from pSM3 & linker
RC   pSM100 from pUB110
RC   pSM17, pSM23 from pSM100 & pSM6 & pSM7
RC   pSM26 from pSM23 & linker
RC   pSM27 from pSM17
RC   pSM29 from pSM23
RC   pSM112 from pSM29
RC   pSM117 from pSM29
RC   pSM115 from pSM112 & pBR322
RC   pSM119 from pSM115 & pSM112
RC   pSM134 from pSM112 & pLV59
RC   pSM135 from pSM134 & pSM112
RA   Grandi G., Del Bue M., Palla E., Mele A., Colletti E., Toma S.;
RT   "New plasmid expression vectors for Bacillus subtilis";
RL   Plasmid 16:1-14(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSM7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pE194)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSM3 large HpaI-SstI/SacI 7320bp,
FT                   \ remove pE194 2142..2901
FT                   EcoRI linker 6bp gaattc:
FT                   2. pE194 HaeIII 3728bp 2614..2614, 120bp 3' to ery RBS
FT                   exonuclease III
FT                   S1 nuclease, remove 120 bp from ends
FT                   SstI/SacI-blunt 160bp,
FT                   \ 156bp 5' to RBS/10bp 3' to ery RBS
FT                   -> pSM7 7480bp [EcoRI starts in RBS]"
FT   -               1..5521
FT                   /note="pSM3 1..5521 5521bp
FT                   \ 232..2141/2901..3728..231
FT                   HpaI =  GTT^AAC
FT                   \           gaattc"
FT   -               5522..5527
FT                   /note="gaattc 6bp
FT                   \        gaattc
FT                   \              ttataaccctctt..."
FT   -               5528..5687
FT                   /note="pE194 2741..2900 160bp
FT                   SacI = SstI = GAGCT^C"
FT   -               5688..7500
FT                   /note="pSM3 6281..8093 1813bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Bacillus pE194"
FT   promoter        0..0
FT                   /note="PRO E. coli ermC gene"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 7500 BP; 2163 A; 1588 C; 1781 G; 1968 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctgc agaacggatt gttgatgatt acgaaaatat taagagcaca gactattaca
     cagaaaatca agaattaaaa aaacgtagag agagtttgaa agaagtagtg aatacatgga
     aagaggggta tcacgaaaaa agtaaagagg ttaataaatt aaagcgagag aatgatagtt
     tgaatgagca gttgaatgta tcagagaaat ttcaagctag tacagtgact ttatatcgtg
     ctgcgagggc gaatttccct gggtttgaga aagggtttaa taggcttaaa gagaaattct
     ttaatgattc caaatttgag cgtgtgggac agtttatgga tgttgtacag gataatgtcc
     agaaggtcga tagaaagcgt gagaaacagc gtacagacga tttagagatg tagaggtact
     tttatgccga gaaaactttt tgcgtgtgac agtccttaaa atatacttag agcgtaagcg
     aaagtagtag cgacagctat taactttcgg ttgcaaagct ctaggatttt taatggacgc
     agcgcatcac acgcaaaaag gaaattggaa taaatgcgaa atttgagatg ttaattaaag
     acctttttga ggtctttttt tcttagattt ttggggttat ttaggggaga aaacataggg
     gggtactacg acctcccccc taggtgtcca ttgtccattg tccaaacaaa taaataaata
     ttgggttttt aatgttaaaa ggttgttttt tatgttaaag tgaaaaaaac agatgttggg
     aggtacagtg atggttgtag atagaaaaga agagaaaaaa gttgctgtta ctttaagact
     tacaacagaa gaaaatgaga tattaaatag aatcaaagaa aaatataata ttagcaaatc
     agatgcaacc ggtattctaa taaaaaaata tgcaaaggag gaatacggtg cattttaaac
     aaaaaaagat agacagcact ggcatgctgc ctatctatga ctaaattttg ttaagtgtat
     tagcaccgtt attatatcat gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat
     tcaagaggac gtaattggac atttgtttta tatccagaat cagcaaaagc cgagtggtta
     gagtatttaa aagagttaca cattcaattt gtagtgtctc cattacatga tagggatact
     gatacagaag gtaggatgaa aaaagagcat tatcatattc tagtgatgta tgagggtaat
     aaatcttatg aacagataaa aataattaac agaagaattg aatgcgacta ttccgcagat
     tgcaggaagt gtgaaaggtc ttgtgagata tatgcttcac atggacgatc ctaataaatt
     taaatatcaa aaagaagata tgatagttta tggcggtgta gatgttgatg aattattaaa
     gaaaacaaca acagatagat ataaattaat taaagaaatg attgagttta ttgatgaaca
     aggaatcgta gaatttaaga gtttaatgga ttatgcaatg aagtttaaat ttgatgattg
     gttcccgctt ttatgtgata actcggcgta tgttattcaa gaatatataa aatcaaatcg
     gtataaatct gaccgataga ttttgaattt aggtgtcaca agacactctt ttttcgcacc
     agcgaaaact ggtttaagcc gactgcgcaa aagacataat cgattcacaa aaaataggca
     cacgaaaaac aagttaaggg atgcagttta tgcatccctt aacttactta ttaaataatt
     tatagctatt gaaaagagat aagaattgtt caaagctaat attgtttaaa tcgtcaattc
     ctgcatgttt taaggaattg ttaaattgat tttttgtaaa tattttcttg tattctttgt
     tgaattctaa ccctctttaa tttggttata tgaattttgc ttattaacga ttcattataa
     ccacttattt tttgtttggt tgataatgaa ctgtgctgat tacaaaaata ctaaaaatgc
     ccatattttt tcctccttat aaaattagta taattatagc acgagctctg ataaatatga
     acatgatgag tgatcgttaa atttatactg caatcggatg cgattattga ataaaagata
     tgagagattt atctaatttc ttttttcttg taaaaaaaga aagttcttaa aggttttata
     gttttggtcg tagagcacac ggtttaacga cttaattacg aagtaaataa gtctagtgtg
     ttagacttta tgaaatctat atacgtttat atatatttat tatccggagg tgtagcatgt
     ctcattcaat tttgagggtt gccagagtta aaggatcaag taatacaaac gggatacaaa
     gacataatca aagagagaat aaaaactata ataataaaga cataaatcat gaggaaacat
     ataaaaatta tgatttgatt aacgcacaaa atataaagta taaagataaa attgatgaaa
     cgattgatga gaattattca gggaaacgta aaattcggtc agatgcaatt cgacatgtgg
     acggactggt tacaagtgat aaagatttct ttgatgattt aagcggagaa gaaatagaac
     gattttttaa agatagcttg gagtttctag aaaatgaata cggtaaggaa aatatgctgt
     atgcgactgt ccatctggat gaaagagtcc cacatatgca ctttggtttt gtccctttaa
     cagaggacgg gagattgtct gcaaaagaac agttaggcaa caagaaagac tttactcaat
     tacaagatag atttaatgag tatgtgaatg agaaaggtta tgaacttgaa agaggcacgt
     ccaaagaggt tacagaacga gaacataaag cgatggatca gtacaagaaa gatactgtat
     ttcataaaca ggaactgcaa gaagttaagg atgagttaca gaaggcaaat aagcagttac
     agagtggaat agagcatatg aggtctacga aaccctttga ttatgaaaat gagcgtacag
     gtttgttctc tggacgtgaa gagactggta gaaagatatt aactgctgat gaatttgaac
     gcctgcaaga aacaatctct tctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattaa
//