ID   PSN2       preliminary; circular DNA; PRO; 1288 BP.
XX
AC   J01763; V01282;
XX
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Staphylococcus plasmid vector pSN2 - complete.
XX
KW   cloning vector.
XX
OS   Staphylococcus aureus
OC   Prokaryotae.
XX
RN   [1]
RP   1-1288
RC   pSA5120-pSN2 from pSN2 & pSA5120
RA   Khan S.A., Novick R.P.;
RT   "Structural analysis of plasmid pSN2 in Staphylococcus aureus: no
RT   involvement in enterotoxin B production";
RL   J. Bacteriol. 149:642-649(1982).
XX
RN   [2]
RC   pSN1 from Staphylococcus aureus, tet gene
RC   pSN2 from Staphylococcus aureus
RC   pSN3 from Staphylococcus aureus, amp gene
RA   Shalita Z., Hertman I., Sarid S.;
RT   "Isolation and characterization of a plasmid involved with
RT   enterotoxin B production in Staphylococcus aureus";
RL   J. Bacteriol. 129:317-325(1977).
XX
CC   Contrary to previous reports, pSN2 doesn't appear to be involved in
CC   enterotoxin B production [1].
CC   Sequence below is inverted and complemented relative to
CC   presentation in [1].
CC   NCBI gi: 151677
CC   NM (pSN2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Staphylococcus)
CC   CP ()
CC   FN ()
CC   SE ()
CC   PA (Staphylococcus)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. Staphylococcus aureus
FT                   -> pSN2 1288bp"
FT   promoter        547..554
FT                   /note="PRO Pribnow box"
FT   RBS             574..579
FT                   /note="RBS Shine-Dalgarno box"
FT   CDS             588..1064
FT                   /note="GEN Staphylococcus 20 kd protein;
FT                   NCBI gi: 151678"
FT   misc_feature    1118..1124
FT                   /note="messenger stop region"
XX
SQ   Sequence 1288 BP; 449 A; 219 C; 248 G; 372 T; 0 other;
     ggcaccaatc gccactgaag tggcatcata ttcgcctttc tttctcaaaa tgttttctcg
     gcataaatgc ttaattaagg tttggcaagc attggctttg ctagccactc aatcgctatc
     gctcattcgt gactgctcgc ctgcttgttg ggggcaagcc cccaaaaccc cctacaaaat
     gctagccata tatcaaaaaa taaacgtaag cataaacgca ttcgcttctt ttctatataa
     atatgagcga agcgaataag cgtcggaaaa gcagcaaaaa gtttcctttt tgctgttgga
     gcatgggggt tcagggggtg cagtatctga cgtcaatgcc gagcgatagc gagccgaagg
     gtagcattta cgttagataa ccccctgata tgctccgacg ctttatatag aaaagaagat
     tcaactaggt aaaatcttaa tataggttga gatgataagg tttataagga atttgtttgt
     agtaattttt gacttgtttt gttgtaattt cttacttgtt ttgttgtatt ttttttacca
     ctgtgataat atttcaacta catactgaaa taagaggtga atataaaatg aaagaaagat
     atggaacagt atataaaggc tctcagaggc tcatagacga ggaaagtgga gaagtaatag
     aggtagataa gctataccgt aaacaaacgt ctggtaactt tgtaaaagcg tatatcgtcc
     aattaataag tatgttagat atgataggcg gtaaaaagct caagattgtt aattatatat
     tagataatgt acatctaagt aataacacaa tgatagcaac tgttagagaa atagcagaag
     gaacaaatac aagcacgaaa accgtaaata caacgcttaa aatcttagaa gaaggaaata
     tcattaaaag aagaactgga gcattaatgc taaacccaga gctactcatg agaggcgatg
     accaaaaaca aaaatacctc ttactcgaat ttgggaactt tgagcaagag gacgaccaaa
     agcaagaaaa tgctttatca gaatattatt ctttcaagga gtagtatagc ataaattcgt
     ctatgttgta gacgaaagga ctagctaaca ctagtccttt tttatatttg tagctttgat
     ctactcgtta cactcgcaga aaaagctaca aataaaagca ataacaaccc accttaatct
     cgctttattc caatttccat tttgagcaga aactttactc atatgatttg gattggttgt
     taagttggaa ttaatccaac ttaaaacc
//