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ID   PSP19      preliminary; circular DNA; SYN; 3011 BP.
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AC   L08946; VB0114; IG0134;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP19 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3011
RC   pSP19 from pBR322 & pUC19 & pGEM-4 & Sp6
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [2]
RC   pSP19 from pBR322 & pUC19 & pGEM-4 & Sp6
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Submitted (00-XXX-1991) on floppy disc by:
RL   BRL, .
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CC   These data and their annotation were supplied to GenBank by Will
CC   Gilbert under the auspices of the GenBank Currator Program.
CC
CC   old BRL vector.
CC   NM (pSP19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pUC19)(pGEM-4)(PromSP6)
CC   BR (pSP18)
CC   OF (pSP6-T7-19)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19c 2686bp, ori/amp gene/MCS
FT                   2. pBR322 remove, 4361bp
FT                   3. pGEM-4 remove, 2871bp
FT                   4. Sp6, promoter
FT                   -> pSP19 3011bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    1..187
FT                   /note="from pUC19c"
FT   misc_feature    complement(187..466)
FT                   /note="from pBR322"
FT   misc_feature    467..691
FT                   /note="from pGEM4"
FT   promoter        692..711
FT                   /note="PRO bacteriophage Sp6"
FT   misc_feature    complement(721..777)
FT                   /note="MCS HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-
FT                   KpnI-SacI-EcoRI; M13mp19/pUC19-polylinker
FT   misc_feature    721..3011
FT                   /note="from pUC19c"
FT   misc_feature    1954..2742
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 3011 BP; 746 A; 776 C; 765 G; 724 T; 0 other;
     tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     accatatcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac
     cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag aaactcagaa
     ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata gggtctgctt
     cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac gcggctacaa
     ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaatacacgg
     gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
     gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     gccctttcgt c
//