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ID   PSP64      preliminary; circular DNA; SYN; 2999 BP.
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AC   X65327; L08943; VB0034; IG0094;
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DT   06-AUG-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP64 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSP64
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2999
RC   pSP64
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pSP64 from pUC12 & SP6 promoter
RC   pSP65 from pUC12 & SP6 promoter
RA   Melton D.A., Krieg P.A., Rebagliati M.R., Maniatis T., Zinn K.,
RA   Green M.R.;
RT   "Efficient in vitro synthesis of biologically active RNA and RNA
RT   hybridization probes from plasmids containing a bacteriophage
RT   SP6 promoter";
RL   Nucleic Acids Res. 12:7035-7056(1984).
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RN   [4]
RC   pSP64
RA   Melton D.A.;
RT   ;
RL   Submitted (00-APR-1986) to GenBank on computer-readable media by:
RL   Melton D.A., Harvard University.
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RN   [5]
RC   pSP64
RA   Gilbert W.;
RT   "Obtained from VecBase 3.0";
RL   Unpublished (1991).
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RN   [6]
RC   pSP6-HbetaA from pSP6-Hbeta
RC   pSP6-HbetaoFA from pSP6-HbetaA & piSVHPbetao
RC   pSP62-PL1 from human beta-globin gene
RA   Green M.R., Maniatis T., Melton D.A.;
RT   "Human beta-globin pre-mRNA synthesized in vitro is accurately
RT   spliced in Xenopus oocyte nuclei";
RL   Cell 32:681-694(1983).
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RN   [7]
RC   R7delta7 from pBR322 & SP6 promoter
RC   pSP6-Hbeta from R7delta7 & human beta-globin gene
RA   Butler E., Little P.;
RT   ;
RL   Unpublished (1983).
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RN   [8]
RC   piSVHPbetao from beta0-thallassemic gene
RA   Treisman R., Proudfoot N.J., Shander M., Maniatis T.;
RT   "A single base change at a splice site in a beta0-thallassemic gene
RT   causes abnormal RNA splicing";
RL   Cell 29:903-911(1982).
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CC   During the construction of the pSP64 vector from pUC12, the
CC   majority of the lacZ gene was deleted.  Therefore. the X-gal
CC   color screening method for the identification of recombinants is
CC   not possible with this vector.
CC   old Boehringer vector.
CC   NM (pSP64)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC   CP (high)
CC   FN (transcription)
CC   SE (color blue/white)
CC   PA (pUC12)(bGal12)(PromSP6)(pBR322)
CC   BR (pSP65)
CC   OF (pGEM-1)(pCKSP6)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove small FnuDII-Sau3A/MboI,
FT                   \ 2680bp-
FT                   \ MboI = 249 399 984 1020 1037 1295 1341 1359 1700
FT                   \ 1805 1817 1895 1903 1914 1989
FT                   \ FnuDII = 30 674 716 1108
FT                   2. Sp6 FnuDII-Sau3A/MboI 400bp, promoter
FT                   -> pSP64 2999bp [no lacZ]"
FT   promoter        1..1
FT                   /note="PRO Salmonella bacteriophage Sp6
FT                   transcription initiation site"
FT   misc_binding    7..57
FT                   /note="MCS HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI-
FT                   EcoRI, from M13mp10/pUC12"
FT   misc_feature    57..286
FT                   /note="E. coli lac operon"
FT   misc_feature    58..2288
FT                   /note="from pUC12 bases 450..2680"
FT   misc_feature    71..71
FT                   /note="E. coli lacZ gene transcription initiation
FT                   site"
FT   promoter        91..107
FT                   /note="PRO E. coli lac operator"
FT   misc_binding    232..232
FT                   /note="SIT unique PvuII"
FT   misc_binding    408..408
FT                   /note="SIT unique AflIII"
FT   rep_origin      <408..<1000
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             1228..2088
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1421..1421
FT                   /note="SIT unique BglI"
FT   misc_binding    1523..1523
FT                   /note="SIT unique AvlII"
FT   misc_binding    1671..1671
FT                   /note="SIT unique PvuII"
FT   misc_binding    1781..1781
FT                   /note="SIT unique ScaI"
FT   misc_binding    1900..1900
FT                   /note="SIT unique Asp700I"
FT   misc_binding    2105..2105
FT                   /note="SIT unique SspI"
FT   misc_binding    2223..2223
FT                   /note="SIT unique AatII"
FT   misc_feature    2289..2475
FT                   /note="from pUC12 bases 1..187"
FT   misc_feature    2522..2800
FT                   /note="from pBR322 bases complement(376..654)"
FT   misc_binding    2567..2567
FT                   /note="SIT unique SphI"
FT   misc_binding    2597..2597
FT                   /note="SIT unique DsaI"
FT   misc_binding    2633..2633
FT                   /note="SIT unique Eco47III"
FT   misc_binding    2726..2726
FT                   /note="SIT unique NaeI"
FT   misc_binding    2757..2757
FT                   /note="SIT unique NheI"
FT   misc_feature    2801..3045
FT                   /note="from bacteriophage Sp6"
FT   promoter        join(2983..2999,1..6)
FT                   /note="PRO bacteriophage Sp6; from Salmonella "
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SQ   Sequence 2999 BP; 744 A; 772 C; 761 G; 722 T; 0 other;
     gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
     atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     gagagtgcac catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
     gcgatgaccc tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga
     aactcagaag gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag
     ggtctgcttc agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg
     cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactata
//