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ID   PSP64F1P   preliminary; circular DNA; SYN; 3513 BP.
XX
AC   ATCC37362;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP64-f1+ - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDM1 from pAT153 & pEMBL8+
RC   pSP64-f1+ from pEMBL8+ & pSP64
RC   pSP64-f1- from pEMBL8+ & pSP64
RC   pSP65-f1+ from pEMBL8+ & pSP65
RC   pSP65-f1- from pEMBL8+ & pSP65
RC   pPTHi74-Sx from pPTHi4 & pPTHi7
RC   pSPTH from pPTHi74-Sx & pSP62-PL1
RC   pUP47 from pSP64-f1- & pSPTH
RC   pPNTH from pUP47 & linker
RA   Mead D.A., Skorupa E.S., Kemper B.;
RT   "Single stranded DNA SP6 promoter plasmids for engineering mutant
RT   RNAs and proteins: synthesis of a 'stretched' preproparathyroid
RT   hormone";
RL   Nucleic Acids Res. 13:1103-1118(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   ssDNA SP6 promoter plasmid.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSP64-f1+)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli NM522)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSP64)(pEMBL8+)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP64 PvuII 2999bp 233..233
FT                   bovine alkaline phosphatase
FT                   2. pEMBL8+ RsaI-RsaI 514bp 771..1285, phage f1 ori
FT                   -> pSP64-f1+ 3513bp"
FT   -               1..232
FT                   /note="pSP64 1..232 232bp
FT                   PvuII = CAG^CTG
FT                   RsaI =   GT^AC"
FT   -               233..746
FT                   /note="pEMBL8+ 771..1284 514bp complement
FT                   RsaI =   GT^AC
FT                   PvuII = CAG^CTG"
FT   -               747..3513
FT                   /note="pSP64 233..2999 2767bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-AccI-HincII-XbaI-
FT                   BamHI-AvaI-SmaI-SacI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-AccI-HincII-
FT                   XbaI-BamHI-AvaI-SmaI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
XX
SQ   Sequence 3513 BP; 907 A; 884 C; 892 G; 830 T; 0 other;
     gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cgaattcgta
     atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agaccccggt
     tgataatcag aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaaa
     cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     gcttaatgcg ccgctacagg gcgcgtctgc attaatgaat cggccaacgc gcggggagag
     gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatc gacgctctcc
     cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
     gaccatttcc ggggtgcgga acggcgttac cagaaactca gaaggttcgt ccaaccaaac
     cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
     cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     gtatcataca catacgattt aggtgacact ata
//