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ID   PSP64POLYA preliminary; circular DNA; SYN; 3033 BP.
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AC   X65328; IG0095;
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DT   28-OCT-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP64 polyA - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSP64 poly(A), revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-3033
RC   pSP64 poly(A)
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pSP64 poly(A) from pSP64
RA   Hoffman L.;
RT   ;
RL   Unpublished (1987).
RL   Hoffman L., Agrigenetics Corp.
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CC   pSP64 poly(A) has 30 dA:dT between the Sac I and Eco RI sites.
CC   It allows a synthetic 3' polyA tail of 30 residues.
CC   The last 3 residues on the 3' end are dCCG.
CC   NM (pSP64 polyA)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli)
CC   CP ()
CC   FN (transcription)
CC   SE (color blue/white)
CC   PA (pSP64)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP64 2999bp, MCS
FT                   2. oligo 34bp, polyA
FT                   -> pSP64 polyA 3033bp"
FT   promoter        1..1
FT                   /note="PRO Salmonella bacteriophage Sp6
FT                   transcription initiation site"
FT   misc_binding    7..91
FT                   /note="MCS HindIII-PstI-SalI-XbaI-BamHI-SmaI-SacI-
FT                   EcoRI, from M13mp10/pUC12"
FT   misc_feature    >50..<86
FT                   /note="poly dA:dT"
FT   misc_feature    91..320
FT                   /note="E. coli lac operon"
FT   misc_feature    105..105
FT                   /note="E. coli lacZ gene transcription initiation
FT                   site"
FT   promoter        125..141
FT                   /note="PRO E. coli lac operator"
FT   misc_binding    266..266
FT                   /note="SIT unique PvuII"
FT   rep_origin      <266..<1000
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             1262..2122
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1455..1455
FT                   /note="SIT unique BglI"
FT   misc_binding    1557..1557
FT                   /note="SIT unique FspI"
FT   misc_binding    1705..1705
FT                   /note="SIT unique PvuI"
FT   misc_binding    1815..1815
FT                   /note="SIT unique ScaI"
FT   misc_binding    1934..1934
FT                   /note="SIT unique XmnI"
FT   misc_binding    2139..2139
FT                   /note="SIT unique SspI"
FT   misc_binding    2257..2257
FT                   /note="SIT unique AatII"
FT   misc_binding    2601..2601
FT                   /note="SIT unique SphI"
FT   misc_binding    2760..2760
FT                   /note="SIT unique NaeI"
FT   misc_binding    2791..2791
FT                   /note="SIT unique NheI"
FT   promoter        join(3017..3033,1..6)
FT                   /note="PRO bacteriophage Sp6; from Salmonella"
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SQ   Sequence 3033 BP; 774 A; 776 C; 761 G; 722 T; 0 other;
     gaatacaagc ttgggctgca ggtcgactct agaggatccc cgggcgagct cccaaaaaaa
     aaaaaaaaaa aaaaaaaaaa aaaccgaatt cgtaatcatg gtcatagctg tttcctgtgt
     gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatc gacgctctcc
     cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
     cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
     tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
     cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
     ggccacgatg cgtccggcgt agaggatctg gctagcgatg accctgctga ttggttcgct
     gaccatttcc ggggtgcgga acggcgttac cagaaactca gaaggttcgt ccaaccaaac
     cgactctgac ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta
     cacaattagg cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat
     gtatcataca catacgattt aggtgacact ata
//