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ID PSP65 preliminary; circular DNA; SYN; 3005 BP.
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AC X65329; L08944; VB0035; IG0096;
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DT 06-AUG-1987 (Rel. 2, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pSP65 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pSP65
RA ;
RT ;
RL Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [2]
RP 1-3005
RC pSP65
RA Klock C.;
RT ;
RL Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [3]
RC pSP64 from pUC12 & SP6 promoter
RC pSP65 from pUC13 & SP6 promoter
RA Melton D.A., Krieg P.A., Rebagliati M.R., Maniatis T., Zinn K.,
RA Green M.R.;
RT "Efficient in vitro synthesis of biologically active RNA and RNA
RT hybridization probes from plasmids containing a bacteriophage
RT SP6 promoter";
RL Nucleic Acids Res. 12:7035-7056(1984).
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RN [4]
RC pSP65 from pUC13 & SP6 promoter
RA Melton D.A.;
RT ;
RL Unpublished (1986).
RL Melton D.A., Harvard University.
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RN [5]
RC pSP65
RA Gilbert W.;
RT "Obtained from VecBase 3.0";
RL Unpublished (1991).
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CC During the construction of the pSP65 vector from pUC12, the
CC majority of the lacZ gene was deleted. Therefore. the X-gal
CC color screening method for the identification of recombinants is
CC not possible with this vector.
CC old Boehringer vector.
CC NM (pSP65)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Promega)
CC HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC CP (high)
CC FN (transcription)
CC SE (color blue/white)
CC PA (pUC13)(bGal13)(PromSP6)(pBR322)
CC BR (pSP64)
CC OF (pGEM-2)
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC13 remove small FnuDII-Sau3A/MboI,
FT \ 2680bp-
FT 2. SP6 FnuDII-Sau3A/MboI 400bp, promoter
FT -> pSP65 3005bp [no lacZ]"
FT promoter 1..1
FT /note="PRO Salmonella bacteriophage Sp6
FT transcription initiation site"
FT misc_binding 10..60
FT /note="MCS EcoRI-SacI-SmaI-BamHI-XbaI-SalI-PstI-
FT HindIII, from M13mp11/pUC13"
FT misc_feature 63..292
FT /note="E. coli lac operon"
FT misc_feature 64..2294
FT /note="from pUC13 bases 450..2680"
FT promoter 97..113
FT /note="PRO E. coli lac operator"
FT misc_feature 77..77
FT /note="E. coli lacZ gene transcription initiation
FT site"
FT misc_binding 238..238
FT /note="SIT unique PvuII"
FT misc_binding 414..414
FT /note="SIT unique AflIII"
FT rep_origin <414..<1000
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 1234..2094
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1427..1427
FT /note="SIT unique BglI"
FT misc_binding 1529..1529
FT /note="SIT unique AvlII"
FT misc_binding 1677..1677
FT /note="SIT unique PvuII"
FT misc_binding 1787..1787
FT /note="SIT unique ScaI"
FT misc_binding 1906..1906
FT /note="SIT unique Asp700I"
FT misc_binding 2111..2111
FT /note="SIT unique SspI"
FT misc_binding 2229..2229
FT /note="SIT unique AatII"
FT misc_feature 2295..2481
FT /note="from pUC13 bases 1..187"
FT misc_feature 2528..2806
FT /note="from pBR322 bases complement(376..654)"
FT misc_binding 2574..2574
FT /note="SIT unique SphI"
FT misc_binding 2603..2603
FT /note="SIT unique DsaI"
FT misc_binding 2639..2639
FT /note="SIT unique Eco47III"
FT misc_binding 2732..2732
FT /note="SIT unique NaeI"
FT misc_binding 2763..2763
FT /note="SIT unique NheI"
FT misc_feature 2807..3005
FT /note="from bacteriophage Sp6"
FT promoter join(2989..3005,1..6)
FT /note="PRO bacteriophage Sp6; from Salmonella"
XX
SQ Sequence 3005 BP; 745 A; 777 C; 761 G; 722 T; 0 other;
gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt
ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
tgtactgaga gtgcaccata tcgacgctct cccttatgcg actcctgcat taggaagcag
cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
tggctagcga tgaccctgct gattggttcg ctgaccattt ccggggtgcg gaacggcgtt
accagaaact cagaaggttc gtccaaccaa accgactctg acggcagttt acgagagaga
tgatagggtc tgcttcagta agccagatgc tacacaatta ggcttgtaca tattgtcgtt
agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
ctata
//