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ID   PSP65F1P   preliminary; circular DNA; SYN; 3519 BP.
XX
AC   ATCC37359;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP65-f1+ - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDM1 from pAT153 & pEMBL8+
RC   pSP64-f1+ from pEMBL8+ & pSP64
RC   pSP64-f1- from pEMBL8+ & pSP64
RC   pSP65-f1+ from pEMBL8+ & pSP65
RC   pSP65-f1- from pEMBL8+ & pSP65
RC   pPTHi74-Sx from pPTHi4 & pPTHi7
RC   pSPTH from pPTHi74-Sx & pSP62-PL1
RC   pUP47 from pSP64-f1- & pSPTH
RC   pPNTH from pUP47 & linker
RA   Mead D.A., Skorupa E.S., Kemper B.;
RT   "Single stranded DNA SP6 promoter plasmids for engineering mutant
RT   RNAs and proteins: synthesis of a 'stretched' preproparathyroid
RT   hormone";
RL   Nucleic Acids Res. 13:1103-1118(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   ssDNA SP6 promoter plasmid.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSP65-f1+)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli NM522)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pSP65)(pEMBL8+)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP65 PvuII 3005bp 239..239
FT                   bovine alkaline phosphatase
FT                   2. pEMBL8+ RsaI-RsaI 514bp 771..1285, phage f1 ori
FT                   -> pSP65-f1+ 3519bp"
FT   -               1..238
FT                   /note="pSP65 1..238 238bp
FT                   PvuII = CAG^CTG
FT                   RsaI =   GT^AC"
FT   -               239..752
FT                   /note="pEMBL8+ 771..1284 514bp
FT                   RsaI =   GT^AC
FT                   PvuII = CAG^CTG"
FT   -               753..3519
FT                   /note="pSP65 239..3005 2767bp"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-AccI-HincII-XbaI-BamHI-
FT                   AvaI-SmaI-SacI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-AccI-HincII-
FT                   XbaI-BamHI-AvaI-SmaI-SacI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
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SQ   Sequence 3519 BP; 853 A; 908 C; 873 G; 885 T; 0 other;
     gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt
     ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagac
     gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac aatcttcctg
     tttttggggc ttttctgatt atcaaccggg gtctgcatta atgaatcggc caacgcgcgg
     ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatcgacg
     ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
     caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc tgctgattgg
     ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag gttcgtccaa
     ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc agtaagccag
     atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat taatacataa
     ccttatgtat catacacata cgatttaggt gacactata
//