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ID   PSP70      preliminary; circular DNA; SYN; 2417 BP.
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AC   X65330; IG0097;
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DT   17-JUN-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP70 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSP70, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2417
RC   pSP70
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pSP66 from pSP64 & linker
RC   pSP67 from pSP65 & linker
RC   pSP68 from pSP64 & linker
RC   pSP69 from pSP65 & linker
RC   pSP70 from pSP64 & linker
RC   pSP71 from pSP65 & linker
RC   pSP72 from pSP64 & linker
RC   pSP73 from pSP65 & linker
RA   Krieg P.A., Melton D.A.;
RT   "In vitro RNA synthesis with SP6 RNA polymerase";
RL   Meth. Enzymol. 155:397-415(1987).
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CC   NM (pSP70)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE ()
CC   PA (pSP64)(pSP65)
CC   BR (pSP71)(pSP72)(pSP73)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP64 remove HindIII-EcoRI 45bp 8..53,
FT                   \ MCS/2954bp
FT                   2. linker XhoI-PvuII-HindIII-EcoRI-ClaI-EcoRV-BglII
FT                   -> pSP70 2417bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    4..45
FT                   /note="MCS XhoI-PvuII-HindIII-EcoRI-ClaI-EcoRV-BglII"
FT   misc_feature    56..56
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        complement(51..73)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    91..91
FT                   /note="SIT unique HpaI"
FT   misc_binding    102..102
FT                   /note="SIT unique BspMI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1090..1950)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1111..1111
FT                   /note="SIT unique BanI"
FT   misc_binding    1283..1283
FT                   /note="SIT unique BglI"
FT   misc_binding    1385..1385
FT                   /note="SIT unique FspI"
FT   misc_binding    1533..1533
FT                   /note="SIT unique PvuI"
FT   misc_binding    1643..1643
FT                   /note="SIT unique ScaI"
FT   misc_binding    1762..1762
FT                   /note="SIT unique XmnI"
FT   misc_binding    1967..1967
FT                   /note="SIT unique SspI"
FT   misc_binding    2085..2085
FT                   /note="SIT unique AatII"
FT   misc_binding    2334..2334
FT                   /note="SIT unique NdeI"
FT   promoter        join(2401..2417,1..6)
FT                   /note="PRO bacteriophage Sp6"
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SQ   Sequence 2417 BP; 615 A; 599 C; 592 G; 611 T; 0 other;
     gaactcgagc agctgaagct tgaattcatc gatgatatca gatctgccgg tctccctata
     gtgagtcgta ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc
     ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
     gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
     cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga
     catattgtcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     atttaggtga cactata
//