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ID   PSP71      preliminary; circular DNA; SYN; 2419 BP.
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AC   X65331; IG0098;
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DT   17-JUN-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP71 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSP71, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2419
RC   pSP71
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pSP66 from pSP64 & linker
RC   pSP67 from pSP65 & linker
RC   pSP68 from pSP64 & linker
RC   pSP69 from pSP65 & linker
RC   pSP70 from pSP64 & linker
RC   pSP71 from pSP65 & linker
RC   pSP72 from pSP64 & linker
RC   pSP73 from pSP65 & linker
RA   Krieg P.A., Melton D.A.;
RT   "In vitro RNA synthesis with SP6 RNA polymerase";
RL   Meth. Enzymol. 155:397-415(1987).
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CC   NM (pSP71)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE ()
CC   PA (pSP64)(pSP65)
CC   BR (pSP70)(pSP72)(pSP73)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP65 remove EcoRI-HindIII 45bp 11..56,
FT                   \ MCS/2960bp
FT                   2. linker BglII-EcoRV-ClaI-EcoRI-HindIII-PvuII-XhoI
FT                   -> pSP71 2419bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    6..47
FT                   /note="MCS BglII-EcoRV-ClaI-EcoRI-HindIII-PvuII-XhoI"
FT   misc_feature    58..58
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        complement(53..75)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    93..93
FT                   /note="SIT unique HpaI"
FT   misc_binding    104..104
FT                   /note="SIT unique BspMI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1092..1952)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1113..1113
FT                   /note="SIT unique BanI"
FT   misc_binding    1285..1285
FT                   /note="SIT unique BglI"
FT   misc_binding    1387..1387
FT                   /note="SIT unique FspI"
FT   misc_binding    1535..1535
FT                   /note="SIT unique PvuI"
FT   misc_binding    1645..1645
FT                   /note="SIT unique ScaI"
FT   misc_binding    1764..1764
FT                   /note="SIT unique XmnI"
FT   misc_binding    1969..1969
FT                   /note="SIT unique SspI"
FT   misc_binding    2087..2087
FT                   /note="SIT unique AatII"
FT   misc_binding    2336..2336
FT                   /note="SIT unique NdeI"
FT   promoter        join(2402..2419,1..6)
FT                   /note="PRO bacteriophage Sp6"
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SQ   Sequence 2419 BP; 615 A; 601 C; 592 G; 611 T; 0 other;
     gaaccagatc tgatatcatc gatgaattca agcttcagct gctcgaggcc ggtctcccta
     tagtgagtcg tattaatttc gataagccag gttaacctgc attaatgaat cggccaacgc
     gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
     cgatttaggt gacactata
//