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ID   PSP72      preliminary; circular DNA; SYN; 2462 BP.
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AC   X65332; IG0099;
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DT   17-JUN-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP72 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSP72, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2462
RC   pSP72
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pSP66 from pSP64 & linker
RC   pSP67 from pSP65 & linker
RC   pSP68 from pSP64 & linker
RC   pSP69 from pSP65 & linker
RC   pSP70 from pSP64 & linker
RC   pSP71 from pSP65 & linker
RC   pSP72 from pSP64 & linker
RC   pSP73 from pSP65 & linker
RA   Krieg P.A., Melton D.A.;
RT   "In vitro RNA synthesis with SP6 RNA polymerase";
RL   Meth. Enzymol. 155:397-415(1987).
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CC   NM (pSP72)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE ()
CC   PA (pSP64)(pSP65)
CC   BR (pSP71)(pSP70)(pSP73)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP70 remove HindIII-EcoRI 6bp 17..23,
FT                   \ MCS/2411bp
FT                   2. pUC18 HindIII-EcoRI 51bp 400..451, MCS
FT                   -> pSP72 2462bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    4..90
FT                   /note="MCS XhoI-PvuII-HindIII-SphI-PstI-SalI-AccI-
FT                   XbaI-BamHI-SmaI-KpnI-SacI-EcoRI-ClaI-EcoRV-BglII"
FT   misc_feature    101..101
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        complement(98..118)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    136..136
FT                   /note="SIT unique HpaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1135..1995)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1328..1328
FT                   /note="SIT unique BglI"
FT   misc_binding    1430..1430
FT                   /note="SIT unique FspI"
FT   misc_binding    1578..1578
FT                   /note="SIT unique PvuI"
FT   misc_binding    1688..1688
FT                   /note="SIT unique ScaI"
FT   misc_binding    1807..1807
FT                   /note="SIT unique XmnI"
FT   misc_binding    2012..2012
FT                   /note="SIT unique SspI"
FT   misc_binding    2130..2130
FT                   /note="SIT unique AatII"
FT   misc_binding    2379..2379
FT                   /note="SIT unique NdeI"
FT   promoter        join(2446..2462,1..6)
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 2462 BP; 623 A; 614 C; 606 G; 619 T; 0 other;
     gaactcgagc agctgaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     gagctcgaat tcatcgatga tatcagatct gccggtctcc ctatagtgag tcgtattaat
     ttcgataagc caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     ctatgcggca tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga
     acgcggctac aattaataca taaccttatg tatcatacac atacgattta ggtgacacta
     ta
//