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ID   PSP73      preliminary; circular DNA; SYN; 2464 BP.
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AC   X65333; IG0100;
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DT   17-JUN-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSP73 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSP73, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2464
RC   pSP73
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pSP66 from pSP64 & linker
RC   pSP67 from pSP65 & linker
RC   pSP68 from pSP64 & linker
RC   pSP69 from pSP65 & linker
RC   pSP70 from pSP64 & linker
RC   pSP71 from pSP65 & linker
RC   pSP72 from pSP64 & linker
RC   pSP73 from pSP65 & linker
RA   Krieg P.A., Melton D.A.;
RT   "In vitro RNA synthesis with SP6 RNA polymerase";
RL   Meth. Enzymol. 155:397-415(1987).
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CC   NM (pSP73)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE ()
CC   PA (pSP64)(pSP65)
CC   BR (pSP71)(pSP70)(pSP72)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSP70 remove HindIII-EcoRI 6bp 17..23,
FT                   \ MCS/2411bp
FT                   2. pUC18 HindIII-EcoRI 51bp 400..451, MCS
FT                   -> pSP73 2464bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    6..92
FT                   /note="MCS BglII-EcoRV-ClaI-EcoRI-SacI-KpnI-SmaI-
FT                   BamHI-XbaI-AccI-SalI-PstI-SphI-HindIII-PvuII-XhoI"
FT   misc_feature    103..103
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        complement(98..120)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    138..138
FT                   /note="SIT unique HpaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1137..1997)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1330..1330
FT                   /note="SIT unique BglI"
FT   misc_binding    1432..1432
FT                   /note="SIT unique FspI"
FT   misc_binding    1580..1580
FT                   /note="SIT unique PvuI"
FT   misc_binding    1690..1690
FT                   /note="SIT unique ScaI"
FT   misc_binding    1809..1809
FT                   /note="SIT unique XmnI"
FT   misc_binding    2014..2014
FT                   /note="SIT unique SspI"
FT   misc_binding    2132..2132
FT                   /note="SIT unique AatII"
FT   misc_binding    2381..2381
FT                   /note="SIT unique NdeI"
FT   promoter        join(2448..2464,1..6)
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 2464 BP; 623 A; 615 C; 607 G; 619 T; 0 other;
     gaaccagatc tgatatcatc gatgaattcg agctcggtac ccggggatcc tctagagtcg
     acctgcaggc atgcaagctt cagctgctcg aggccggtct ccctatagtg agtcgtatta
     atttcgataa gccaggttaa cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     aactatgcgg catcagagca gattgtactg agagtgcacc atatggacat attgtcgtta
     gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     tata
//