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ID PSPORT1 preliminary; circular DNA; SYN; 4109 BP.
XX
AC IG0137;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pSPORT1 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pSPORT1 from pUC series
RA Crouse J.E.;
RT "Plasmid pSPORT1";
RL GibcoBRL Catalogue 93:7p15-7p16(1993).
RL Crouse J.E., Life Technologies Inc., Gaithersburg MD 20877.
XX
CC NM (pSPORT1)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (BRL)
CC HO (E.coli)
CC CP ()
CC FN (expression)(cDNA cloning random/oriented)(sequencing dideoxy)
CC FN (in vitro mutagenesis)
CC SE (color blue/white)
CC PA (pUC)
CC BR (pSV-SPORT1)(pSPORT2)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC18 2686bp
FT 2. E. coli 1400bp, lacI gene
FT -> pSPORT1 4109bp"
FT CDS complement(0..<550)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT primer_bind 125..147
FT /note="PRI bacteriophage M13 23-base forward"
FT primer_bind 140..156
FT /note="PRI bacteriophage M13 forward"
FT promoter 164..183
FT /note="PRO bacteriophage Sp6"
FT misc_binding 191..191
FT /note="SIT unique AatII"
FT misc_binding 197..208
FT /note="MCS AatII-SphI-MluI-SplI-SnaBI-HindIII-BamHI-
FT XbaI-NotI-XmaIII-SpeI-SstI-SalI-SmaI-EcoRI-BspMII-
FT RsrII-KpnI-PstI"
FT misc_binding 280..280
FT /note="SIT unique PstI"
FT promoter complement(298..318)
FT /note="PRO bacteriophage T7"
FT primer_bind 333..347
FT /note="PRI bacteriophage T7T3alpha"
FT promoter complement(>1500..<1700)
FT /note="PRO E. coli lacUV5 gene"
FT CDS complement(>300..<1600)
FT /note="REP E. coli lacI repressor gene"
FT misc_binding 552..552
FT /note="SIT unique NarI"
FT misc_binding 582..582
FT /note="SIT unique Esp3I"
FT misc_binding 743..743
FT /note="SIT unique EcoRV"
FT misc_binding 984..984
FT /note="SIT unique ApaI"
FT misc_binding 1009..1009
FT /note="SIT unique BstEII"
FT misc_binding 1177..1177
FT /note="SIT unique BclI"
FT promoter complement(>1500..<1700)
FT /note="PRO E. coli lacI gene"
FT misc_binding 1610..1610
FT /note="SIT unique PflMI"
FT rep_origin complement(>1648..<1841)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT misc_binding 2252..2252
FT /note="SIT unique AlwNI"
FT CDS complement(>2500..3536)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 3212..3212
FT /note="SIT unique ScaI"
FT misc_binding 3250..3250
FT /note="SIT unique BcgI"
FT misc_binding 3329..3329
FT /note="SIT unique XmnI"
FT rep_origin 3667..4109
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_binding 3872..3872
FT /note="SIT unique DraIII"
FT misc_binding 3978..3978
FT /note="SIT unique NseI"
XX
SQ Sequence 4109 BP; 1002 A; 1064 C; 1062 G; 981 T; 0 other;
cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata
gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gcggccgccg
actagtgagc tcgtcgaccc gggaattccg gaccggtacc tgcaggcgta ccagctttcc
ctatagtgag tcgtattaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
gcaggcgaaa atcctgtttg atggtggttg acggcgggat ataacatgag ctgtcttcgg
tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acccgttgcg
cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
tgtcaacgta aatgccgctt cgccttcgcg cgcgaattgc aagctctgca ttaatgaatc
ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta
aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
gcgcttaatg cgccgctaca gggcgcgtc
//