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ID   PSPORT1    preliminary; circular DNA; SYN; 4109 BP.
XX
AC   IG0137;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSPORT1 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSPORT1 from pUC series
RA   Crouse J.E.;
RT   "Plasmid pSPORT1";
RL   GibcoBRL Catalogue 93:7p15-7p16(1993).
RL   Crouse J.E., Life Technologies Inc., Gaithersburg MD 20877.
XX
CC   NM (pSPORT1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (BRL)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cDNA cloning random/oriented)(sequencing dideoxy)
CC   FN (in vitro mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC)
CC   BR (pSV-SPORT1)(pSPORT2)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp
FT                   2. E. coli 1400bp, lacI gene
FT                   -> pSPORT1 4109bp"
FT   CDS             complement(0..<550)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     125..147
FT                   /note="PRI bacteriophage M13 23-base forward"
FT   primer_bind     140..156
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        164..183
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    191..191
FT                   /note="SIT unique AatII"
FT   misc_binding    197..208
FT                   /note="MCS AatII-SphI-MluI-SplI-SnaBI-HindIII-BamHI-
FT                   XbaI-NotI-XmaIII-SpeI-SstI-SalI-SmaI-EcoRI-BspMII-
FT                   RsrII-KpnI-PstI"
FT   misc_binding    280..280
FT                   /note="SIT unique PstI"
FT   promoter        complement(298..318)
FT                   /note="PRO bacteriophage T7"
FT   primer_bind     333..347
FT                   /note="PRI bacteriophage T7T3alpha"
FT   promoter        complement(>1500..<1700)
FT                   /note="PRO E. coli lacUV5 gene"
FT   CDS             complement(>300..<1600)
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    552..552
FT                   /note="SIT unique NarI"
FT   misc_binding    582..582
FT                   /note="SIT unique Esp3I"
FT   misc_binding    743..743
FT                   /note="SIT unique EcoRV"
FT   misc_binding    984..984
FT                   /note="SIT unique ApaI"
FT   misc_binding    1009..1009
FT                   /note="SIT unique BstEII"
FT   misc_binding    1177..1177
FT                   /note="SIT unique BclI"
FT   promoter        complement(>1500..<1700)
FT                   /note="PRO E. coli lacI gene"
FT   misc_binding    1610..1610
FT                   /note="SIT unique PflMI"
FT   rep_origin      complement(>1648..<1841)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    2252..2252
FT                   /note="SIT unique AlwNI"
FT   CDS             complement(>2500..3536)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    3212..3212
FT                   /note="SIT unique ScaI"
FT   misc_binding    3250..3250
FT                   /note="SIT unique BcgI"
FT   misc_binding    3329..3329
FT                   /note="SIT unique XmnI"
FT   rep_origin      3667..4109
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    3872..3872
FT                   /note="SIT unique DraIII"
FT   misc_binding    3978..3978
FT                   /note="SIT unique NseI"
XX
SQ   Sequence 4109 BP; 1002 A; 1064 C; 1062 G; 981 T; 0 other;
     cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata
     gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gcggccgccg
     actagtgagc tcgtcgaccc gggaattccg gaccggtacc tgcaggcgta ccagctttcc
     ctatagtgag tcgtattaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     gcaggcgaaa atcctgtttg atggtggttg acggcgggat ataacatgag ctgtcttcgg
     tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg
     cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acccgttgcg
     cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     tgtcaacgta aatgccgctt cgccttcgcg cgcgaattgc aagctctgca ttaatgaatc
     ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc
     tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta
     aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     gcgcttaatg cgccgctaca gggcgcgtc
//