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ID   PSPORT2    preliminary; circular DNA; SYN; 4310 BP.
XX
AC   IG1114;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pSPORT2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSPORT2 from pUC series
RA   Crouse J.E.;
RT   "Plasmid pSPORT2";
RL   GibcoBRL Catalogue 93:7p15-7p15(1993).
RL   Crouse J.E., Life Technologies Inc., Gaithersburg MD 20877.
XX
CC   NM (pSPORT2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (BRL)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cDNA cloning random/oriented)(sequencing dideoxy)
CC   FN (in vitro mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC)
CC   BR (pSV-SPORT1)(pSPORT1)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSPORT1 BspHI/BspI 4109bp 2562..2562,
FT                   \ may be 3570
FT                   2. lambda BspHI/BspI 48502bp 40643..40643,
FT                   \ loxP gene
FT                   -> plasmid 52611bp
FT                   1. plasmid BspHI/BspI 3570..3570, 4109bp+loxP
FT                   2. phage P1 BspHI/BspI-BspHI/BspI, incA gene
FT                   -> pSPORT2 4310bp"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 23-base forward"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    0..0
FT                   /note="SIT unique AatII"
FT   misc_binding    0..0
FT                   /note="MCS AatII-SphI-MluI-SplI-SnaBI-HindIII-BamHI-
FT                   XbaI-NotI-XmaIII-SpeI-SstI-SalI-SmaI-EcoRI-BspMII-
FT                   RsrII-KpnI-PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage T7T3alpha"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacUV5 gene"
FT   CDS             complement(0..0)
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   misc_binding    0..0
FT                   /note="SIT unique Esp3I"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI"
FT   promoter        complement(0..0)
FT                   /note="PRO E. coli lacI gene"
FT   misc_binding    0..0
FT                   /note="SIT unique PflMI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT unique AlwNI"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique ScaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BcgI"
FT   misc_binding    0..0
FT                   /note="SIT unique XmnI"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    0..0
FT                   /note="SIT unique DraIII"
FT   misc_binding    0..0
FT                   /note="SIT unique NseI"
XX
SQ   Sequence 4310 BP; 1059 A; 1126 C; 1096 G; 1029 T; 0 other;
     cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     ttttcccagt cacgacgttg taaaacgacg gccagtgaat tgaatttagg tgacactata
     gaagagctat gacgtcgcat gcacgcgtac gtaagcttgg atcctctaga gtcgacgagc
     tcactagtga cagcggccgc cccgggaatt ccggaccggt acctgcaggc gtaccagctt
     tccctatagt gagtcgtatt agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt
     cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacccgtt
     gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     tggtgtcaac gtaaatgccg cttcgccttc gcgcgcgaat tgcaagctct gcattaatga
     atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
     tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc ataacttcgt
     atagcataca ttatacgaag ttatggcatg agattatcaa aaaggatctt cacctagatc
     cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     cagggttatt gtctcatgcc aggggtgggc acacatattt gataccagcg atccctacac
     agcacataat tcaatgcgac ttccctctat cgcacatctt agacctttat tctccctcca
     gcacacatcg aagctgccga gcaagccgtt ctcaccagtc caagacctgg catgagcgga
     tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
//