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ID   PSPT18     preliminary; circular DNA; SYN; 3104 BP.
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AC   IG0171; A13388; A13387;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSPT18 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSPT18 from pUC18 & T7 & Sp6
RA   Hoeltke H.J., Seibl R., Schmitz G., Schoeler H.R., Kessler C.,
RA   Mattes,R.;
RT   "Method for the detection of nucleic acids";
RL   Unpublished (1992).
RL   European Patent Number 0324474-A 4 dated 19-JUL-1989.
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CC   NM (pSPT18)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)
CC   HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC   CP (high)
CC   FN (transcription both strands)
CC   SE ()
CC   PA (pUC18)(bacteriophage T7)(Salmonella bacteriophage Sp6)
CC   BR (pSPT19)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC18 2686bp, ori/amp gene/MCS
FT                   2. Sp6, promoter
FT                   3. T7, promoter
FT                   -> pSPT18 3104bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    1..72
FT                   /note="MCS HindIII-SphI-PstI-BspMI-SalI-XbaI-
FT                   BamHI-SmaI-KpnI-SacI-EcoRI"
FT   promoter        complement(65..81)
FT                   /note="PRO bacteriophage T7"
FT   primer_bind     complement(65..81)
FT                   /note="PRI bacteriophage M13"
FT   misc_feature    72..72
FT                   /note="bacteriophage T7 transcription initiation site"
FT   misc_binding    104..104
FT                   /note="SIT unique PvuII"
FT   misc_feature    111..248
FT                   /note="homologous to bases 2395..2532"
FT   misc_binding    260..260
FT                   /note="SIT unique AspI"
FT   misc_binding    267..267
FT                   /note="SIT unique BsaAI"
FT   misc_feature    291..341
FT                   /note="homologous to bases 2532..2582"
FT   misc_binding    338..338
FT                   /note="SIT unique NdeI"
FT   misc_binding    515..515
FT                   /note="SIT unique AflIII"
FT   rep_origin      >800..<1330
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1336..2195)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1630..1630
FT                   /note="SIT unique AvlII"
FT   misc_binding    1778..1778
FT                   /note="SIT unique PvuII"
FT   misc_binding    1888..1888
FT                   /note="SIT unique ScaI"
FT   misc_binding    2007..2007
FT                   /note="SIT unique Asp700I"
FT   misc_binding    2212..2212
FT                   /note="SIT unique SspI"
FT   misc_binding    2330..2330
FT                   /note="SIT unique AatII"
FT   misc_binding    2384..2384
FT                   /note="SIT unique DraII"
FT   misc_binding    2387..2387
FT                   /note="SIT unique PssI"
FT   misc_binding    2704..2704
FT                   /note="SIT unique DsaI"
FT   misc_binding    2739..2739
FT                   /note="SIT unique Eco47III"
FT   misc_binding    2832..2832
FT                   /note="SIT unique NaeI"
FT   misc_binding    2863..2863
FT                   /note="SIT unique NheI"
FT   promoter        3065..3084
FT                   /note="PRO bacteriophage Sp6"
FT   primer_bind     3065..3084
FT                   /note="PRI bacteriophage M13"
XX
SQ   Sequence 3104 BP; 768 A; 800 C; 802 G; 734 T; 0 other;
     gaatacaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa
     ttccggtctc cctatagtga gtcgtattaa tttcgataag ccagctgggc ctcgcgcgtt
     tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatata ctggcttaac
     tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     atcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     cggccacggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg
     agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc
     gccggtgatg ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct
     gattggttcg ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg
     tccaaccaaa ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa
     gccagatgct acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata
     cataacctta tgtatcatac acatacgatt taggtgacac tata
//