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ID   PSPT19     preliminary; circular DNA; SYN; 3107 BP.
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AC   IG0172;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSPT19 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSPT19 from pUC19 & T7 & Sp6
RA   Hoeltke H.J., Seibl R., Schmitz G., Schoeler H.R., Kessler C.,
RA   Mattes,R.;
RT   "Method for the detection of nucleic acids";
RL   Unpublished (1992).
RL   European Patent Number 0324474-A 4 dated 19-JUL-1989.
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CC   NM (pSPT19)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)
CC   HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC   CP (high)
CC   FN (transcription both strands)
CC   SE ()
CC   PA (pUC19)(bacteriophage T7)(Salmonella bacteriophage Sp6)
CC   BR (pSPT18)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp, ori/amp gene/MCS
FT                   2. Sp6, promoter
FT                   3. T7, promoter
FT                   -> pSPT19 3107bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    1..75
FT                   /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT                   BspMI-PstI-SphI-HindIII"
FT   promoter        complement(68..84)
FT                   /note="PRO bacteriophage T7"
FT   primer_bind     complement(68..84)
FT                   /note="PRI bacteriophage M13"
FT   misc_feature    75..75
FT                   /note="bacteriophage T7 transcription initiation site"
FT   misc_binding    107..107
FT                   /note="SIT unique PvuII"
FT   misc_feature    114..251
FT                   /note="homologous to bases 2395..2532"
FT   misc_binding    263..263
FT                   /note="SIT unique AspI"
FT   misc_binding    270..270
FT                   /note="SIT unique BsaAI"
FT   misc_feature    294..344
FT                   /note="homologous to bases 2532..2582"
FT   misc_binding    341..341
FT                   /note="SIT unique NdeI"
FT   misc_binding    518..518
FT                   /note="SIT unique AflIII"
FT   rep_origin      >800..<1330
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1339..2198)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1633..1633
FT                   /note="SIT unique AvlII"
FT   misc_binding    1781..1781
FT                   /note="SIT unique PvuII"
FT   misc_binding    1891..1891
FT                   /note="SIT unique ScaI"
FT   misc_binding    2010..2010
FT                   /note="SIT unique Asp700I"
FT   misc_binding    2215..2215
FT                   /note="SIT unique SspI"
FT   misc_binding    2333..2333
FT                   /note="SIT unique AatII"
FT   misc_binding    2387..2387
FT                   /note="SIT unique DraII"
FT   misc_binding    2390..2390
FT                   /note="SIT unique PssI"
FT   misc_binding    2707..2707
FT                   /note="SIT unique DsaI"
FT   misc_binding    2742..2742
FT                   /note="SIT unique Eco47III"
FT   misc_binding    2835..2835
FT                   /note="SIT unique NaeI"
FT   misc_binding    2866..2866
FT                   /note="SIT unique NheI"
FT   promoter        3068..3087
FT                   /note="PRO bacteriophage Sp6"
FT   primer_bind     3068..3087
FT                   /note="PRI bacteriophage M13"
XX
SQ   Sequence 3107 BP; 769 A; 799 C; 805 G; 734 T; 0 other;
     gaatacacgg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     aagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagctg ggcctcgcgc
     gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat atactggctt
     aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     ccccggccac gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctggctag cgatgaccct
     gctgattggt tcgctgacca tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt
     tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag
     taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta
     atacataacc ttatgtatca tacacatacg atttaggtga cactata
//