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ID PSPT19 preliminary; circular DNA; SYN; 3107 BP.
XX
AC IG0172;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pSPT19 - complete.
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KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pSPT19 from pUC19 & T7 & Sp6
RA Hoeltke H.J., Seibl R., Schmitz G., Schoeler H.R., Kessler C.,
RA Mattes,R.;
RT "Method for the detection of nucleic acids";
RL Unpublished (1992).
RL European Patent Number 0324474-A 4 dated 19-JUL-1989.
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CC NM (pSPT19)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Boehringer)
CC HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC CP (high)
CC FN (transcription both strands)
CC SE ()
CC PA (pUC19)(bacteriophage T7)(Salmonella bacteriophage Sp6)
CC BR (pSPT18)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC19 2686bp, ori/amp gene/MCS
FT 2. Sp6, promoter
FT 3. T7, promoter
FT -> pSPT19 3107bp"
FT misc_feature 1..1
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT misc_binding 1..75
FT /note="MCS EcoRI-SacI-KpnI-SmaI-BamHI-XbaI-SalI-
FT BspMI-PstI-SphI-HindIII"
FT promoter complement(68..84)
FT /note="PRO bacteriophage T7"
FT primer_bind complement(68..84)
FT /note="PRI bacteriophage M13"
FT misc_feature 75..75
FT /note="bacteriophage T7 transcription initiation site"
FT misc_binding 107..107
FT /note="SIT unique PvuII"
FT misc_feature 114..251
FT /note="homologous to bases 2395..2532"
FT misc_binding 263..263
FT /note="SIT unique AspI"
FT misc_binding 270..270
FT /note="SIT unique BsaAI"
FT misc_feature 294..344
FT /note="homologous to bases 2532..2582"
FT misc_binding 341..341
FT /note="SIT unique NdeI"
FT misc_binding 518..518
FT /note="SIT unique AflIII"
FT rep_origin >800..<1330
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1339..2198)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1633..1633
FT /note="SIT unique AvlII"
FT misc_binding 1781..1781
FT /note="SIT unique PvuII"
FT misc_binding 1891..1891
FT /note="SIT unique ScaI"
FT misc_binding 2010..2010
FT /note="SIT unique Asp700I"
FT misc_binding 2215..2215
FT /note="SIT unique SspI"
FT misc_binding 2333..2333
FT /note="SIT unique AatII"
FT misc_binding 2387..2387
FT /note="SIT unique DraII"
FT misc_binding 2390..2390
FT /note="SIT unique PssI"
FT misc_binding 2707..2707
FT /note="SIT unique DsaI"
FT misc_binding 2742..2742
FT /note="SIT unique Eco47III"
FT misc_binding 2835..2835
FT /note="SIT unique NaeI"
FT misc_binding 2866..2866
FT /note="SIT unique NheI"
FT promoter 3068..3087
FT /note="PRO bacteriophage Sp6"
FT primer_bind 3068..3087
FT /note="PRI bacteriophage M13"
XX
SQ Sequence 3107 BP; 769 A; 799 C; 805 G; 734 T; 0 other;
gaatacacgg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
aagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagctg ggcctcgcgc
gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat atactggctt
aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
ccccggccac gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atctggctag cgatgaccct
gctgattggt tcgctgacca tttccgggtg cgggacggcg ttaccagaaa ctcagaaggt
tcgtccaacc aaaccgactc tgacggcagt ttacgagaga gatgataggg tctgcttcag
taagccagat gctacacaat taggcttgta catattgtcg ttagaacgcg gctacaatta
atacataacc ttatgtatca tacacatacg atttaggtga cactata
//