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ID   PSPTBM20   preliminary; circular DNA; SYN; 3140 BP.
XX
AC   IG0173;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSPTbm20 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSPTbm20 from pSPT18
RA   Hoeltke H.J., Seibl R., Schmitz G., Schoeler H.R., Kessler C.,
RA   Mattes,R.;
RT   "Method for the detection of nucleic acids";
RL   Unpublished (1992).
RL   European Patent Number 0324474-A 4 dated 19-JUL-1989.
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CC   NM (pSPTbm20)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)
CC   HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC   CP (high)
CC   FN (transcription both strands)
CC   SE ()
CC   PA (pSPT18)
CC   BR (pSPTbm21)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSPT18 remove HindIII-EcoRI 51bp 8..59,
FT                   \ MCS/3053bp
FT                   2. oligo HindIII-EcoRI 87bp, MCS
FT                   -> pSPTbm20 3140bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    1..131
FT                   /note="MCS HindIII-NcoI-EcoRV-SphI-PstI-SacI-XbaI-
FT                   SalI-ApaI-KpnI-KspI-EclXI-NotI-BfrI-MluI-BamHI-SmaI-
FT                   EcoRI"
FT   promoter        complement(109..125)
FT                   /note="PRO bacteriophage T7"
FT   primer_bind     complement(109..125)
FT                   /note="PRI bacteriophage M13"
FT   misc_feature    114..114
FT                   /note="bacteriophage T7 transcription initiation site"
FT   misc_binding    140..140
FT                   /note="SIT unique PvuII"
FT   misc_binding    296..296
FT                   /note="SIT unique AspI"
FT   misc_binding    374..374
FT                   /note="SIT unique NdeI"
FT   rep_origin      >400..<800
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1372..2231)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1666..1666
FT                   /note="SIT unique AvlII"
FT   misc_binding    1814..1814
FT                   /note="SIT unique PvuI"
FT   misc_binding    1924..1924
FT                   /note="SIT unique ScaI"
FT   misc_binding    2043..2043
FT                   /note="SIT unique Asp700I"
FT   misc_binding    2248..2248
FT                   /note="SIT unique SspI"
FT   misc_binding    2366..2366
FT                   /note="SIT unique AatII"
FT   misc_binding    2420..2420
FT                   /note="SIT unique DraII"
FT   misc_binding    2775..2775
FT                   /note="SIT unique Eco47III"
FT   misc_binding    2868..2868
FT                   /note="SIT unique NaeI"
FT   misc_binding    2899..2899
FT                   /note="SIT unique NheI"
FT   promoter        3123..3140
FT                   /note="PRO bacteriophage Sp6"
FT   primer_bind     3123..3140
FT                   /note="PRI bacteriophage M13"
XX
SQ   Sequence 3140 BP; 774 A; 812 C; 815 G; 739 T; 0 other;
     gaatacaagc ttccatggga tatcgcatgc ctgcagagct ctagagtcga cgggcccggt
     accgcggccg ccttaagacg cgtggatccc cgggaattcc ggtctcccta tagtgagtcg
     tattaatttc gataagccag ctgggcctcg cgcgtttcgg tgatgacggt gaaaacctct
     gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     cacgtagcga tagcggagtg tatatactgg cttaactatg cggcatcaga gcagattgta
     ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     gcggcatcag agcagattgt actgagagtg caccatatcg acgctctccc ttatgcgact
     cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacgggcctg ccaccatacc
     cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
     gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
     tccggcgtag aggatctggc tagcgatgac cctgctgatt ggttcgctga ccatttccgg
     gtgcgggacg gcgttaccag aaactcagaa ggttcgtcca accaaaccga ctctgacggc
     agtttacgag agagatgata gggtctgctt cagtaagcca gatgctacac aattaggctt
     gtacatattg tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
     acgatttagg tgacactata
//