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ID   PSPTBM21   preliminary; circular DNA; SYN; 3143 BP.
XX
AC   IG0174;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSPTbm21 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSPTbm21 from pSPT19
RA   Hoeltke H.J., Seibl R., Schmitz G., Schoeler H.R., Kessler C.,
RA   Mattes,R.;
RT   "Method for the detection of nucleic acids";
RL   Unpublished (1992).
RL   European Patent Number 0324474-A 4 dated 19-JUL-1989.
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CC   NM (pSPTbm21)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Boehringer)
CC   HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC   CP (high)
CC   FN (transcription both strands)
CC   SE ()
CC   PA (pSPT19)
CC   BR (pSPTbm20)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSPT19 remove EcoRI-HindIII 51bp 11..62,
FT                   \ MCS/3056bp
FT                   2. oligo EcoRI-HindIII 87bp, MCS
FT                   -> pSPTbm21 3143bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    1..134
FT                   /note="MCS EcoRI-SmaI-BamHI-MluI-BfrI-NotI-EclXI-
FT                   KspI-KpnI-ApaI-SalI-XbaI-SacI-PstI-SphI-EcoRV-NcoI-
FT                   HindIII"
FT   promoter        complement(112..128)
FT                   /note="PRO bacteriophage T7"
FT   primer_bind     complement(112..128)
FT                   /note="PRI bacteriophage M13"
FT   misc_feature    117..117
FT                   /note="bacteriophage T7 transcription initiation site"
FT   misc_binding    143..143
FT                   /note="SIT unique PvuII"
FT   misc_binding    299..299
FT                   /note="SIT unique AspI"
FT   misc_binding    377..377
FT                   /note="SIT unique NdeI"
FT   rep_origin      >400..<800
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1375..2234)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1669..1669
FT                   /note="SIT unique AvlII"
FT   misc_binding    1817..1817
FT                   /note="SIT unique PvuI"
FT   misc_binding    1927..1927
FT                   /note="SIT unique ScaI"
FT   misc_binding    2046..2046
FT                   /note="SIT unique Asp700I"
FT   misc_binding    2251..2251
FT                   /note="SIT unique SspI"
FT   misc_binding    2369..2369
FT                   /note="SIT unique AatII"
FT   misc_binding    2423..2423
FT                   /note="SIT unique DraII"
FT   misc_binding    2778..2778
FT                   /note="SIT unique Eco47III"
FT   misc_binding    2871..2871
FT                   /note="SIT unique NaeI"
FT   misc_binding    2902..2902
FT                   /note="SIT unique NheI"
FT   promoter        3126..3143
FT                   /note="PRO bacteriophage Sp6"
FT   primer_bind     3126..3143
FT                   /note="PRI bacteriophage M13"
XX
SQ   Sequence 3143 BP; 774 A; 816 C; 813 G; 740 T; 0 other;
     gaatacacgg aattccccgg gatccacgcg tcttaaggcg gccgcggtac ccggcccgtc
     gactctagag ctctgcaggc atgcgatatc ccatggaagc ttgggtctcc ctatagtgag
     tcgtattaat ttcgataagc cagctgggcc tcgcgcgttt cggtgatgac ggtgaaaacc
     tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     agtcacgtag cgatagcgga gtgtatatac tggcttaact atgcggcatc agagcagatt
     gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     cggcgagcgg tatcagctca ctcaaaggcc ctaatacggt tatccacaga atcaggggat
     aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     tatgcggcat cagagcagat tgtactgaga gtgcaccata tcgacgctct cccttatgcg
     actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggc ctgccaccat
     acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc tgaccatttc
     cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac cgactctgac
     ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta cacaattagg
     cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca
     catacgattt aggtgacact ata
//