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ID PSPTBM21 preliminary; circular DNA; SYN; 3143 BP.
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AC IG0174;
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DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pSPTbm21 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pSPTbm21 from pSPT19
RA Hoeltke H.J., Seibl R., Schmitz G., Schoeler H.R., Kessler C.,
RA Mattes,R.;
RT "Method for the detection of nucleic acids";
RL Unpublished (1992).
RL European Patent Number 0324474-A 4 dated 19-JUL-1989.
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CC NM (pSPTbm21)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Boehringer)
CC HO (E.coli HB101)(E.coli LE392)(E.coli JM83)
CC CP (high)
CC FN (transcription both strands)
CC SE ()
CC PA (pSPT19)
CC BR (pSPTbm20)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pSPT19 remove EcoRI-HindIII 51bp 11..62,
FT \ MCS/3056bp
FT 2. oligo EcoRI-HindIII 87bp, MCS
FT -> pSPTbm21 3143bp"
FT misc_feature 1..1
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT misc_binding 1..134
FT /note="MCS EcoRI-SmaI-BamHI-MluI-BfrI-NotI-EclXI-
FT KspI-KpnI-ApaI-SalI-XbaI-SacI-PstI-SphI-EcoRV-NcoI-
FT HindIII"
FT promoter complement(112..128)
FT /note="PRO bacteriophage T7"
FT primer_bind complement(112..128)
FT /note="PRI bacteriophage M13"
FT misc_feature 117..117
FT /note="bacteriophage T7 transcription initiation site"
FT misc_binding 143..143
FT /note="SIT unique PvuII"
FT misc_binding 299..299
FT /note="SIT unique AspI"
FT misc_binding 377..377
FT /note="SIT unique NdeI"
FT rep_origin >400..<800
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1375..2234)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1669..1669
FT /note="SIT unique AvlII"
FT misc_binding 1817..1817
FT /note="SIT unique PvuI"
FT misc_binding 1927..1927
FT /note="SIT unique ScaI"
FT misc_binding 2046..2046
FT /note="SIT unique Asp700I"
FT misc_binding 2251..2251
FT /note="SIT unique SspI"
FT misc_binding 2369..2369
FT /note="SIT unique AatII"
FT misc_binding 2423..2423
FT /note="SIT unique DraII"
FT misc_binding 2778..2778
FT /note="SIT unique Eco47III"
FT misc_binding 2871..2871
FT /note="SIT unique NaeI"
FT misc_binding 2902..2902
FT /note="SIT unique NheI"
FT promoter 3126..3143
FT /note="PRO bacteriophage Sp6"
FT primer_bind 3126..3143
FT /note="PRI bacteriophage M13"
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SQ Sequence 3143 BP; 774 A; 816 C; 813 G; 740 T; 0 other;
gaatacacgg aattccccgg gatccacgcg tcttaaggcg gccgcggtac ccggcccgtc
gactctagag ctctgcaggc atgcgatatc ccatggaagc ttgggtctcc ctatagtgag
tcgtattaat ttcgataagc cagctgggcc tcgcgcgttt cggtgatgac ggtgaaaacc
tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
agtcacgtag cgatagcgga gtgtatatac tggcttaact atgcggcatc agagcagatt
gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
cggcgagcgg tatcagctca ctcaaaggcc ctaatacggt tatccacaga atcaggggat
aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
tatgcggcat cagagcagat tgtactgaga gtgcaccata tcgacgctct cccttatgcg
actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggc ctgccaccat
acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
gcgtccggcg tagaggatct ggctagcgat gaccctgctg attggttcgc tgaccatttc
cgggtgcggg acggcgttac cagaaactca gaaggttcgt ccaaccaaac cgactctgac
ggcagtttac gagagagatg atagggtctg cttcagtaag ccagatgcta cacaattagg
cttgtacata ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca
catacgattt aggtgacact ata
//