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ID   PSS24      preliminary; circular DNA; SYN; 4375 BP.
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AC   ATCC37391;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSS24 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSS24 from pSV1 or pBR322SV & pD4, f1 ori
RC   pSS25 from pSS24 & linker
RC   pMN from pSS25 & pSS24
RC   pMX from pSV1 or pBR322SV & pSS24
RA   Kowalski J., Smith J.H., Ng N., Denhardt D.T.;
RT   "Vectors for the direct selection of cDNA clones corresponding to
RT   mammalian cell mRNA of low abundance";
RL   Gene 35:45-54(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   The f1 intergenic region for packaging of ssDNA was inserted into an
CC   Okayama & Berg vector.
CC   Plasmid vector for constructing cDNA libraries.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSS24)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli RR1)(E.coli)(E.coli R4)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SV40)(pD4)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove HindIII-PvuII 2037bp 30..2067,
FT                   \ tet/2324bp
FT                   2. SV40 PvuII-HindIII 774bp 273..1047,
FT                   \ late polyA/0.71 to 0.86 3722..4509 [787bp]
FT                   -> pBR322-SV40 3102bp
FT                   1. pBR322-SV40 EcoRI 3102bp,
FT                   \ pBR322 4360..4360
FT                   2. pD4 EcoRI-? 1260bp 4292..5552, phage f1 ori
FT                   \ [1248bp]
FT                   -> plasmid 4362bp
FT                   1. plasmid Tth111I 4362bp, pBR322 2223..2223
FT                   Klenow
FT                   BamHI linker 10bp nnggatccnn
FT                   -> pSS24 4373bp"
FT   -               1..157
FT                   /note="pBR322 2067..2223 157bp
FT                   Tth111I = GACN^N NGTC
FT                   \                nnggatccnn"
FT   -               158..167
FT                   /note="nnggatccnn 10bp
FT                   \   nnggatccnn
FT                   Tth111I = GACN^NNGTC"
FT   -               168..2304
FT                   /note="pBR322 2223..4359 2137bp
FT                   EcoRI = G^AATTC"
FT   -               2305..3566
FT                   /note="pD4 4292..5553 1262bp
FT                   \    ...g
FT                   EcoRI = G^AATTC"
FT   -               3567..3597
FT                   /note="pBR322 4360..4361..29 31bp
FT                   HindIII = A^AGCTT"
FT   -               3598..4375
FT                   /note="SV40 273..1050 778bp complement
FT                   PvuII = CAG^CTG"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-HindIII-PvuII-BamHI-HpaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
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SQ   Sequence 4375 BP; 1134 A; 1063 C; 990 G; 1184 T; 4 other;
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagnng gatccnngtc acgtagcgat
     agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaattca ttgagcgtca aaatgtaggt atttccatga
     gcgtttttcc tgttgcaatg gctggcggta atattgttct ggatattacc agcaaggccg
     atagtttgag ttcttctact caggcaagtg atgttattac taatcaaaga agtattgcga
     caacggttaa tttgcgtgat ggacagactc ttttactcgg tggcctcact gattataaaa
     acacttctca ggattctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt
     ttagctcccg ctctgattct aacgaggaaa gcacgttata cgtgctcgtc aaagcaacca
     tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     tttaacgcga attttaacaa aatattaacg tttacaattt aaatatttgc ttatacaatc
     ttcctgtttt tggggctttt ctgattatca accggggtac atatgattga catgctagtt
     ttacgattac cgttcatcga ttctcttgtt tgctccagac tctcaggcaa tgacctgata
     gcctttgtag acctctcaaa aatagctacc ctctccggca tgaatttatc agctagaacg
     gttgaatatc atattgatgg tgatttgact gtctccggcc tttctcaccc gtttgaatct
     ttacctacac attactcagg cattgcattt aaaatatatg agggttctaa aaatttttat
     ccttgcgttg aaataaaggc ttctcccgca aaagtattac agggtcataa tgtttttggt
     acaaccgatt tagctttatg ctctgaggct ttattgctta attttgctaa ttctttgcct
     tgcctgtatg atttattgga tgttagaatt ctcatgtttg acagcttatc atcgataagc
     ttgagaaatg gcattaaaaa gtgttggacc ccaatgtctg gggtcaagat actgaacact
     gtgaacaaag gtttgtactc caggaaataa aatatcatag taatcatctg gcctatacaa
     atctacagcc attcctggtt gttgatataa accaacagta gaaactttgt gatcccagtc
     actaaaaaat ctatacccca cttgagcaac agcgctcaca ccagtcacag tttgcagtaa
     agctgcaaat ccagctatag cagcaggagc cccagatatc acagcatagg cctgtggagt
     gaggcctata gcagcaattg cctcagaggt tgttaggcct tcaacagtag caacagatgc
     aagttgcact tcaattgcag cagcggcctc tccagcagca atttcagcta ctgaaaatcc
     agtagcagca gcagcttcag acacagtagc aattaggtcc cccaacagtg ttaaagcagc
     acccatggac ctgaaataaa agacaaaaag actaaactta ccagttaact ttctggtttt
     tcagttaacc tttctggttt tttgcgtttc ccgtcaacag tatcttcccc ttcacaaaat
     tgcagcaaaa gctctaaaac aaacacaaaa aggcgttgag ctgttttttt actttcagtc
     catgacctac gaaccttaac ggaggcctgg cgtgacagcc ggcgcagcac catggcctga
     aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accag
//