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ID   PSS25      preliminary; circular DNA; SYN; 4389 BP.
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AC   ATCC37392;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pSS25 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSS24 from pSV1 or pBR322SV & pD4, f1 ori
RC   pSS25 from pSS24 & linker
RC   pMN from pSS25 & pSS24
RC   pMX from pSV1 or pBR322SV & pSS24
RA   Kowalski J., Smith J.H., Ng N., Denhardt D.T.;
RT   "Vectors for the direct selection of cDNA clones corresponding to
RT   mammalian cell mRNA of low abundance";
RL   Gene 35:45-54(1985).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   Plasmid vector for construction of cDNA libraries.
CC   The f1 intergenic region for packaging of ssDNA was inserted into an
CC   Okayama & Berg vector. SV40 sequences have been removed.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pSS25)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli R4)(E.coli)(E.coli RR1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(SV40)(pD4)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSS24 HindIII 4350bp, pBR322 30..30
FT                   T4 DNA polymerase:T4 DNA polymerase
FT                   bacterial alkaline phosphatase
FT                   KpnI linker 10bp nnggtaccnn
FT                   KpnI
FT                   -> pSS25 4352bp"
FT   -               1..3601
FT                   /note="pSS24 1..3601 3601bp
FT                   HindIII = A^AGCT T
FT                   \                nnggtaccnn"
FT   -               3602..3611
FT                   /note="nnggtaccnn 10bp
FT                   \ nnggtaccnn
FT                   HindIII =  A^AGCTT"
FT   -               3612..4389
FT                   /note="pSS24 3598..4375 778bp complement"
FT   misc_binding    0..0
FT                   /note="SIT unique KpnI-PvuII-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
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SQ   Sequence 4389 BP; 1136 A; 1066 C; 993 G; 1186 T; 8 other;
     ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     gggtgttggc gggtgtcggg gcgcagccat gacccagnng gatccnngtc acgtagcgat
     agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagaattca ttgagcgtca aaatgtaggt atttccatga
     gcgtttttcc tgttgcaatg gctggcggta atattgttct ggatattacc agcaaggccg
     atagtttgag ttcttctact caggcaagtg atgttattac taatcaaaga agtattgcga
     caacggttaa tttgcgtgat ggacagactc ttttactcgg tggcctcact gattataaaa
     acacttctca ggattctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt
     ttagctcccg ctctgattct aacgaggaaa gcacgttata cgtgctcgtc aaagcaacca
     tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     tttaacgcga attttaacaa aatattaacg tttacaattt aaatatttgc ttatacaatc
     ttcctgtttt tggggctttt ctgattatca accggggtac atatgattga catgctagtt
     ttacgattac cgttcatcga ttctcttgtt tgctccagac tctcaggcaa tgacctgata
     gcctttgtag acctctcaaa aatagctacc ctctccggca tgaatttatc agctagaacg
     gttgaatatc atattgatgg tgatttgact gtctccggcc tttctcaccc gtttgaatct
     ttacctacac attactcagg cattgcattt aaaatatatg agggttctaa aaatttttat
     ccttgcgttg aaataaaggc ttctcccgca aaagtattac agggtcataa tgtttttggt
     acaaccgatt tagctttatg ctctgaggct ttattgctta attttgctaa ttctttgcct
     tgcctgtatg atttattgga tgttagaatt ctcatgtttg acagcttatc atcgataagc
     tnnggtaccn nagcttgaga aatggcatta aaaagtgttg gaccccaatg tctggggtca
     agatactgaa cactgtgaac aaaggtttgt actccaggaa ataaaatatc atagtaatca
     tctggcctat acaaatctac agccattcct ggttgttgat ataaaccaac agtagaaact
     ttgtgatccc agtcactaaa aaatctatac cccacttgag caacagcgct cacaccagtc
     acagtttgca gtaaagctgc aaatccagct atagcagcag gagccccaga tatcacagca
     taggcctgtg gagtgaggcc tatagcagca attgcctcag aggttgttag gccttcaaca
     gtagcaacag atgcaagttg cacttcaatt gcagcagcgg cctctccagc agcaatttca
     gctactgaaa atccagtagc agcagcagct tcagacacag tagcaattag gtcccccaac
     agtgttaaag cagcacccat ggacctgaaa taaaagacaa aaagactaaa cttaccagtt
     aactttctgg tttttcagtt aacctttctg gttttttgcg tttcccgtca acagtatctt
     ccccttcaca aaattgcagc aaaagctcta aaacaaacac aaaaaggcgt tgagctgttt
     ttttactttc agtccatgac ctacgaacct taacggaggc ctggcgtgac agccggcgca
     gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
     aagaaccag
//