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ID   PSTNEO     preliminary; circular DNA; SYN; 5663 BP.
XX
AC   IG9915;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pSTneo - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pSTneo from pSV2-neo & HSV tk gene & linker
RC   pSTneoA from pSTneo & pIBI76 & linker
RC   pSTneoB from pSTneoA
RA   Katoh K., Takahashi Y., Hayashi S., Kondoh H.;
RT   "Improved mammalian vectors for high expression of G418 resistance";
RL   Cell Struct. Funct. 12:575-580(1987).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pSTneo)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(teratocarcinoma cells)(embryonic stem cells)
CC   HO (vertebrate cells)
CC   CP ()
CC   FN ()
CC   SE ()
CC   PA (pSV2neo)(pIBI76 from IBI)(HSV-1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pSV2-neo remove HindIII-BglII 320bp
FT                   \ 2773..3093, neo gene/5409bp
FT                   2. HSV-1 tk prom PvuII-BglII 260bp 47860..48108
FT                   \  -157 to +56/#X14112 [249bp]
FT                   HindIII linker 6bp aagctt:
FT                   -> pSTneo 5669bp"
FT   -               1..2772
FT                   /note="pSV2-neo 1..2772 2772bp
FT                   HindIII = A^AGCTT
FT                   \           agctt"
FT   -               2773..2777
FT                   /note="agctt 5bp
FT                   \    aagctt
FT                   PvuII = CAG^CTG"
FT   -               2778..3026
FT                   /note="HSV-1 47860..48108 249bp complement
FT                   BglII = A^GATCT"
FT   -               3027..5663
FT                   /note="pSV2-neo 3093..5729 2637bp"
FT   promoter        0..0
FT                   /note="PRO HSV tk gene"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli neomycin phosphotransferase II gene
FT                   (NPTII), neomycin resistance gene (neo),
FT                   from HindIII-BamHI fragment of transposon Tn5"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early gene"
FT   misc_feature    0..0
FT                   /note="SPL SV40 intervening sequence
FT                   with splice acceptor"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 5663 BP; 1459 A; 1392 C; 1409 G; 1403 T; 0 other;
     aattcctttg cctaatttaa atgaggactt aacctgtgga aatattttga tgtgggaagc
     tgttactgtt aaaactgagg ttattggggt aactgctatg ttaaacttgc attcagggac
     acaaaaaact catgaaaatg gtgctggaaa acccattcaa gggtcaaatt ttcatttttt
     tgctgttggt ggggaacctt tggagctgca gggtgtgtta gcaaactaca ggaccaaata
     tcctgctcaa actgtaaccc caaaaaatgc tacagttgac agtcagcaga tgaacactga
     ccacaaggct gttttggata aggataatgc ttatccagtg gagtgctggg ttcctgatcc
     aagtaaaaat gaaaacacta gatattttgg aacctacaca ggtggggaaa atgtgcctcc
     tgttttgcac attactaaca cagcaaccac agtgcttctt gatgagcagg gtgttgggcc
     cttgtgcaaa gctgacagct tgtatgtttc tgctgttgac atttgtgggc tgtttaccaa
     cacttctgga acacagcagt ggaagggact tcccagatat tttaaaatta cccttagaaa
     gcggtctgtg aaaaacccct acccaatttc ctttttgtta agtgacctaa ttaacaggag
     gacacagagg gtggatgggc agcctatgat tggaatgtcc tctcaagtag aggaggttag
     ggtttatgag gacacagagg agcttcctgg ggatccagac atgataagat acattgatga
     gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat
     tccttattaa cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta
     ttaatagcag acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa
     ctgttatgcc tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt
     gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca
     gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc
     ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc
     atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca
     tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat
     tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
     aattatgtca caccacagaa gtaaggttcc ttcacaaaga tccgggacca aagcggccat
     cgtgcctccc cactcctgca gttcgggggc atggatgcgc ggatagccgc tgctggtttc
     ctggatgccg acggatttgc actgccggta gaactccgcg aggtcgtcca gcctcaggca
     gcagctgaac caactcgcga ggggatcgag cccggggtgg gcgaagaact ccagcatgag
     atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     gtctcttgat caagcttctg cttcatcccc gtggcccgtt gctcgcgttt gctggcggtg
     tccccggaag aaatatattt gcatgtcttt agttctatga tgacacaaac cccgcccagc
     gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg ggcggcgcgg tcccaggtcc
     acttcgcata ttaaggtgac gcgtgtggcc tcgaataccg agcgaccctg cagcgacccg
     cttaacagcg tcaacagcgt gccgcaagct ttttgcaaaa gcctaggcct ccaaaaaagc
     ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct gcataaataa
     aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga
     tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac
     ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca
     tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca
     cattccacag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat
     cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga
     taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     cacgaggccc tttcgtcttc aag
//