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ID   PSU23      preliminary; circular DNA; SYN; 2397 BP.
XX
AC   X53937;
XX
DT   18-SEP-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pSU23 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2397
RC   [pSU1017 from pSU series]
RC   pSU2799 from pSU2713
RC   pSU2798 from pSU2799 & pHSG399
RC   pSU8 from pUC8 & pSU2798
RC   [pSU5 from pSU8]
RC   pSU9 from pUC9 & pSU2798
RC   pSU18 from pUC18 & pSU2798
RC   pSU19 from pUC19 & pSU2798
RC   pSU20 from pHSG396 & pSU19
RC   pSU21 from pHSG397 & pSU19
RC   pSU2751 from pSU1017 & pSU2799
RC   pSU2752 from pSU2751 & pK18
RC   pSU36 from pSU2752 & pUC8
RC   pSU37 from pSU2752 & pUC9
RC   pSU38 from pSU2752 & pUC18
RC   pSU39 from pSU2752 & pUC19
RC   pSU40 from pHSG396 & pSU39
RC   pSU41 from pHSG397 & pSU39
RC   pSU23 from pSU2798 & pGEM-3Z
RC   pSU24 from pSU2798 & pGEM-4Z
RA   Bartolome B., Jubete Y., Martinez E., De La Cruz F.;
RT   "Construction and properties of a family of pACYC184-derived cloning
RT   vectors compatible with pBR322 and its derivatives";
RL   Gene 102:75-78(1991).
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RN   [2]
RP   1-2397
RC   pSU23
RA   Bartolome B.;
RT   ;
RL   Submitted (16-JUL-1990) on tape to the EMBL Data Library by:
RL   Bartolome B., University of Cantabria, Dpto. Molecular Biology,
RL   Cardenal Herrera Oria s/n., Santander 39001, Spain.
XX
CC   NM (pSU23)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pACYC184)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 remove HaeII-HaeII 1774bp
FT                   \ 1732..3506, except cat/2471bp
FT                   -> pSU2703 2471bp [unique HindIII, XbaI, cat gene]
FT                   1. pSU2703 remove XbaI-BanI 581bp
FT                   \ 1426..1732 & 3506..3781, 1890bp [577bp]
FT                   -> pSU2713 1890bp [1893bp; unique HaeII]
FT                   1. pSU2713 AspI 1890bp 3703..3703
FT                   Klenow
FT                   -> pSU2799 1890bp
FT                   1. pSU2799 RsaI-RsaI 1352bp, p15A ori
FT                   \ pACYC184 127..1426..-..3781..3834 [1357bp]
FT                   2. pHSG399 RsaI-RsaI 538bp 286..824,
FT                   \ Tn9 cat gene [no EcoRI]
FT                   -> pSU2798 1895bp [unique HaeII]
FT                   1. pGEM-3Z HaeII-HaeII 370bp 324..694,
FT                   \ lacZ gene/MCS/Sp6/T7
FT                   2. pSU2798 HaeII 1895bp, pHSG399 1774..1774
FT                   -> pSU23 2397bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2397 BP; 612 A; 598 C; 583 G; 604 T; 0 other;
     cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
     accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgcctaga aatattttat
     ctgattaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga
     aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt
     gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc
     ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca
     gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc
     cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
     gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt
     atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt
     aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg
     ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta
     tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca
     ctgacaccct catcagtgcc aacatagtaa gccagtatat acactccgct agcgcccaat
     acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca
     ctatagaata ctcaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga
     gctcgaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc
     gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     tgaatggcga atggcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag
     tagctgaaca ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc
     atcatacact aaatcagtaa gttggcagca tcacccgacg cactttgcgc cgaataaata
     cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg
     aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac
     tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca
     ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattt
//