back Return to this vector's summary.
ID   PSU2718    preliminary; circular DNA; SYN; 2338 BP.
XX
AC   M64731;
XX
DT   25-MAY-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSU2718 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSU2703 from pACYC184
RC   pSU2713 from pSU2703
RC   pSU2716 from pSU2713 & pUC8
RC   pSU2717 from pSU2713 & pUC9
RC   pSU2718 from pSU2713 & pUC18
RC   pSU2719 from pSU2713 & pUC19
RA   Martinez E., Bartolome B., De La Cruz F.;
RT   "pACYC184-derived cloning vectors containing the multiple cloning
RT   site and lacZ-alpha reporter gene of pUC8/9 and pUC18/19 plasmids";
RL   Gene 68:159-162(1988).
XX
RN   [2]
RP   1-2338
RC   from pACYC177
RC   from pACYC184
RC   from pSU2718
RC   from pSU2719
RA   Chandler M.S.;
RT   "New shuttle vectors for Haemophilus influenzae and Escherichia
RT   coli: P15A-derived plasmids replicate in H. influenzae Rd";
RL   Plasmid 25:221-224(1991).
XX
RN   [3]
RC   pGJB101 from pHVT1
RC   pGJB45 from pHCV5 & linker
RC   pGJB103 from pGJB101 & pGJB45
RC   pDALK from pGJB103 & omega & H.influenzae & Tn10, kan gene
RC   pDAL from pDALK
RA   Tomb J.F., Barcak G.J., Chandler M.S., Redfield R.J., Smith H.O.;
RT   "Transposon mutagenesis, characterization, and cloning of
RT   transformation genes of Haemophilus influenzae Rd";
RL   J. Bacteriol. 171:3796-3802(1989).
XX
RN   [4]
RC   pHCV5 from pBR322 & RSF007
RC   pHVT1 from pBR322 & RSF0855
RA   Danner D.B., Pifer M.L.;
RT   "Plasmid cloning vectors resistant to ampicillin and tetracycline
RT   which can replicate in both E. coli and Haemophilus cells";
RL   Gene 18:101-105(1982).
XX
CC   NM (pSU2718)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(H. influenzae Rd)
CC   CP ()
CC   FN (cloning)(shuttle)
CC   SE (color blue/white)
CC   PA (pACYC184)(pUC18)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 remove HaeII-HaeII 1774bp
FT                   \ 1732..3506, except cat/2471bp
FT                   -> pSU2703 2471bp [unique HindIII, XbaI, cat gene]
FT                   1. pSU2703 remove XbaI-BanI 581bp
FT                   \ 1426..1732 & 3506..3781, 1890bp [577bp]
FT                   -> pSU2713 1890bp [1893bp; unique HaeII]
FT                   1. pSU2713 HaeII 1890bp
FT                   2. pUC18 HaeII-HaeII 445bp 240..685, lacZ gene [448bp]
FT                   -> pSU2718 2338bp"
FT   CDS             complement(1..219)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_feature    1..584
FT                   /note="from pACYC184, bases 1..584"
FT   misc_feature    complement(584..1874)
FT                   /note="BanI-HaeII fragment of transposon Tn9"
FT   misc_feature    585..1029
FT                   /note="HaeII fragment of pUC18; contains
FT                   lacZ-alpha and MCS"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   misc_feature    1030..1873
FT                   /note="from pACYC184, bases 585..1428"
FT   rep_origin      1030..1873
FT                   /note="ORI E. coli miniplasmid p15A"
FT   misc_feature    1874..2338
FT                   /note="from pACYC184, bases 3780..4244"
FT   CDS             complement(1898..2338)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
XX
SQ   Sequence 2338 BP; 584 A; 575 C; 586 G; 593 T; 0 other;
     gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
     ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
     gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
     acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgccattcg ccattcaggc
     tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     gttgtaaaac gacggccagt gccaagcttg catgcctgca ggtcgactct agaggatccc
     cgggtaccga gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     attgggcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
     aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
     caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
     cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
     catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     caggcgtttc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
     gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
     ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
     agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
     agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
     aaaatatttc taggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
     cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca cagacggcat
     gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacg
//