back Return to this vector's summary.
ID   PSU36      preliminary; circular DNA; SYN; 2664 BP.
XX
AC   X53938;
XX
DT   18-SEP-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pSU36 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-2664
RC   [pSU1017 from pSU series]
RC   pSU2799 from pSU2713
RC   pSU2798 from pSU2799 & pHSG399
RC   pSU8 from pUC8 & pSU2798
RC   [pSU5 from pSU8]
RC   pSU9 from pUC9 & pSU2798
RC   pSU18 from pUC18 & pSU2798
RC   pSU19 from pUC19 & pSU2798
RC   pSU20 from pHSG396 & pSU19
RC   pSU21 from pHSG397 & pSU19
RC   pSU2751 from pSU1017 & pSU2799
RC   pSU2752 from pSU2751 & pK18
RC   pSU36 from pSU2752 & pUC8
RC   pSU37 from pSU2752 & pUC9
RC   pSU38 from pSU2752 & pUC18
RC   pSU39 from pSU2752 & pUC19
RC   pSU40 from pHSG396 & pSU39
RC   pSU41 from pHSG397 & pSU39
RC   pSU23 from pSU2798 & pGEM-3Z
RC   pSU24 from pSU2798 & pGEM-4Z
RA   Bartolome B., Jubete Y., Martinez E., De La Cruz F.;
RT   "Construction and properties of a family of pACYC184-derived cloning
RT   vectors compatible with pBR322 and its derivatives";
RL   Gene 102:75-78(1991).
XX
RN   [2]
RP   1-2664
RC   pSU36
RA   Bartolome B.;
RT   ;
RL   Submitted (16-JUL-1990) on tape to the EMBL Data Library by:
RL   Bartolome B., University of Cantabria, Dpto. Molecular Biology,
RL   Cardenal Herrera Oria s/n., Santander 39001, Spain.
XX
CC   NM (pSU36)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pACYC184)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 remove HaeII-HaeII 1774bp
FT                   \ 1732..3506, except cat/2471bp
FT                   -> pSU2703 2471bp [unique HindIII, XbaI, cat gene]
FT                   1. pSU2703 remove XbaI-BanI 581bp
FT                   \ 1426..1732 & 3506..3781, 1890bp [577bp]
FT                   -> pSU2713 1890bp [1893bp, unique HaeII]
FT                   1. pACYC184 HindIII-SmaI 1323bp 1525 1196..2519,
FT                   \ kan gene
FT                   -> pSU1017
FT                   1. pSU2713 AspI 1890bp, pACYC184 3703..3703
FT                   Klenow
FT                   -> pSU2799 1890bp
FT                   1. pSU2799 BanI-PvuII 915bp, p15A ori
FT                   Klenow:Klenow
FT                   2. pSU1017 HindIII-SmaI 1323bp, kan gene
FT                   \ Tn5 1196..2519 [1322bp]
FT                   Klenow:Klenow
FT                   -> pSU2751 2237bp
FT                   1. pSU2751 NcoI-BglII 1644bp
FT                   2. pK18 BglII-NcoI 596bp 330..926,
FT                   \ Tn5 neo gene [no SphI-PstI]
FT                   -> pSU2752 2240bp
FT                   1. pSU2752 HaeII 2240bp, not in kan gene
FT                   2. pUC8 HaeII-HaeII 424bp 5..429, lacZ gene/MCS
FT                   -> pSU36 2664bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 2664 BP; 588 A; 781 C; 710 G; 585 T; 0 other;
     gtgcctagaa atattttatc tgattaataa gatgatcttc ttgagatcgt tttggtctgc
     gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc
     tctgagctac caactctttg aaccgaggta actggcttgg aggagcgcag tcaccaaaac
     ttgtcctttc agtttagcct taaccggcgc atgacttcaa gactaactcc tctaaatcaa
     ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt ccgggttgga ctcaagacga
     tagttaccgg ataaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca
     taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     gccaggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg
     gccctctcac ttccctgtta agtatcttcc tggcatcttc caggaaatct ccgccccgtt
     cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
     aagcggaata tatcctgtat cacatattct gctgacgcac cggtgcagcc ttttttctcc
     tgccacatga agcacttcac tgacaccctc atcagtgcca acatagtaag ccagtatata
     cactccgcta gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     aattcccggg gatccgtcga cctgcagcca agcttggcac tggccgtcgt tttacaacgt
     cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     ctgaatggcg aatggcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca
     gtagctgaac aggagggaca gggggtgggc gaagaactcc agcatgagat ccccgcgctg
     gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     tcgccatggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag cctggcgaac
     agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     agccacgata gccgcgctgc ctcgtcttgg agttcattca gggcaccgga caggtcggtc
     ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
     cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
     cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
     tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     caccgtttct gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct
     gagtgcttgc ggcagcgtga agct
//